ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

Endocrine Abstracts (2013) 32 P504 | DOI: 10.1530/endoabs.32.P504

Effects of chlorobenzenes on the histamine (HA) activated arginine-vasopressin (AVP) release [ndash] from rat pituitary cells

Zsuzsanna Valkusz1, Zsolt Molnar2, Peter Hausinger2, Mariann Radacs2, Marta Galfi2, Regina Palfoldi3 & Bela Jojart4


1Department of Endocrinology, University of Szeged, Szeged, Hungary; 2Institute of Applied Natural Science, Faculty of Education, Department of Environmental Biology and Education, Szeged, Hungary; 3Department of Pulmonology, Szeged, Hungary; 4Department of Chemicals Informatics, Szeged, Hungary.


Introduction: The endocrine disruptor compounds (EDC), as persistent organic pollutant (POP) agents, have ability to interfere with endocrine systems. These properties of EDC may modify the homeostasis of biological organisms.

Aim: The aim was to investigate the effects of chlorobenzenes (ClB), hexa-chlorobenzenes+2,4,6,trichloro-benzenes, with chronic exposure and extremely low doses on histamine mediated neuroendocrine functions.

Methods: The model-animals, Wistar male rats (bw: 80–200 g), were exposed to ClB (1.0 μg/bw kg per day) via a gastric tube for 0, 30, 60, and 90 days. After the ClB treatment, organs and bodyweight, furthermore the hepatic transferase enzymes of Wistar rats were measured. At the completion of the in vivo treatment plasma AVP was determined by RIA, and pituicytes from the rat neurohypophyses were cultured. HA-stimulated AVP release was investigated in the cell cultures. The statistical analyses were performed by ANOVA.

Results: AVP secretion was increased by HA stimulation as compared to control (124.57±1.39 pg AVP/mg prot) vs (43.29±1.39 pg AVP/mg prot). These AVP results demonstrate that the neuro-endocrine function was significantly modulated by a chronic and subtoxical ClB treatment.

Conclusion: It can be hypothesised that ClBs may influence homeostatic processes of exposed animals through endocrine chanels.

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