Introduction: The endocrine disruptor compounds (EDC), as persistent organic pollutant (POP) agents, have ability to interfere with endocrine systems. These properties of EDC may modify the homeostasis of biological organisms.
Aim: The aim was to investigate the effects of chlorobenzenes (ClB), hexa-chlorobenzenes+2,4,6,trichloro-benzenes, with chronic exposure and extremely low doses on histamine mediated neuroendocrine functions.
Methods: The model-animals, Wistar male rats (bw: 80200 g), were exposed to ClB (1.0 μg/bw kg per day) via a gastric tube for 0, 30, 60, and 90 days. After the ClB treatment, organs and bodyweight, furthermore the hepatic transferase enzymes of Wistar rats were measured. At the completion of the in vivo treatment plasma AVP was determined by RIA, and pituicytes from the rat neurohypophyses were cultured. HA-stimulated AVP release was investigated in the cell cultures. The statistical analyses were performed by ANOVA.
Results: AVP secretion was increased by HA stimulation as compared to control (124.57±1.39 pg AVP/mg prot) vs (43.29±1.39 pg AVP/mg prot). These AVP results demonstrate that the neuro-endocrine function was significantly modulated by a chronic and subtoxical ClB treatment.
Conclusion: It can be hypothesised that ClBs may influence homeostatic processes of exposed animals through endocrine chanels.
27 Apr - 01 May 2013
European Society of Endocrinology