Endocrine Abstracts (2013) 32 P372 | DOI: 10.1530/endoabs.32.P372

Association between toll-like receptor 4 polymorphism and type 2 diabetes mellitus

Banu Mesci1, Pinar Eren2, Safak Kiziltas1, Aytekin Oguz1, Ilyas Tuncer1, Osman Kostek1 & Yasar Colak1


1Internal Medicine Department, Goztepe Training and Research Hospital, Istanbul Medeniyet University, Istanbul, Turkey; 2Genetic Department, Haydarpasa Numune Education and Research Hospital, Istanbul, Turkey.


Introduction: Type two diabetes (T2DM), is the most common form of diabetes and known to be associated with low-grade inflammation. Since TLR-4 plays an important role in bacteriel recognition of lipopolysaccharide (LPS) and activation of proinflammatory pathways we aimed of to test whether TLR-4 gene polymorphism plays a protective role in the development of T2DM in a pilot Turkish population.

Description of methods/design: Blood samples were collected from 58 patients with T2DM and 63 healthy people. Allelic variants of TLR-4 (Asp299Gly and Thr399Ile) were assayed by real-time PCR. Genomic DNA was amplified using FAM/VIC primers spesific for allelic variants of TLR-4 Asp299Gly (refSNP ID: rs4986790) and Thr399Ile (refSNP ID: rs4986791) with real-time PCR. Amplicons were analyzed with high resolution melting at Light Cycler 480 for detecting different melting patterns of polymorphic and wild type alleles.

Results and conclusion: For genotype 299 gen polymorphism; 17.2% of the patients (n=10) with T2DM were heterozygotic (Asp299Gly), 6.8% (n=4) of them were homozygotic (Gly299Gly) mutant. 9.5% (n=6) of the controls were heterozygotic and 6.3% (n=4) of them were homozygotic. Heterozygotic and homozygotic mutation between patients with T2DM and controls were similar (P=0.21, P=0.9 respectively)For genotype 399 gen polymorphism; 8.6% of the patients (n=5) with T2DM and 9.5% (n=4) of the controls were heterozygotic (Thr299Ile). None of them were homozygotic (Ile299Ile) mutant. Heterozygotic mutation between patients with T2DM and controls were similar (P=0.63). The cumulative mutations number of the subjects with TLR-4 gene polymorphism were. similar in groups (17 vs 12, P=0.18). Our results demonstrated that TLR-4 gene polymorphism has no association with T2DM.

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