Searchable abstracts of presentations at key conferences in endocrinology
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Society for Endocrinology BES 2014

Poster Presentations


ea0034p315 | Reproduction | SFEBES2014

Protective role of Nigerian honey on sperm indices and testis in sucrose-fed rat

Oyelowo Oluwakemi , Adekunbi Daniel , Dada Kayode

This study aimed at investigating the effect of high sucrose diet on male reproductive function and if Nigerian honey could exert a protective role. Twenty-four rats were randomly divided into four equal groups of six animals and given water (control); honey (H); high sucrose solution (30%w/v) (HSS); and both high sucrose solution (30%w/v) and honey (HSS+H). Each rat on honey received a daily dose of 10 ml honey/kg per 5 ml of distilled water. Food intake, body weight, organ w...

ea0034p316 | Reproduction | SFEBES2014

Effects of bovine somatotropin on libido, serum testosterone, hematology and certain biochemical metabolites of Sahiwal bulls

Dilbar Ghulam Hussain , Ahmad Nazir , Ahmad Maqbool , Ahmad Ijaz , Waqas Salman , Younis Muhammad

In this study, effects of bovine somatotropin on libido, serum testosterone, hematological indices and certain serum biochemical metabolites in Sahiwal bulls were investigated. Six adult Sahiwal bulls with clinically normal reproductive tract and kept at the Semen Production Unit, Qadirabad, Sahiwal, Pakistan were used. These bulls were divided into two equal groups, i.e. treatment and control. Bulls of the treatment group were given bovine somatotropin (bST) at of 500 mg week...

ea0034p317 | Reproduction | SFEBES2014

Gestational diabetes and polycystic ovary syndrome

Georgiou Christiana , Mason Helen , Bano Gul

Gestational diabetes mellitus (GDM) poses multiple risks to both the mother and foetus. This includes foetal macrosomia and the development of type 2 diabetes mellitus (T2DM). Women with polycystic ovary syndrome (PCOS) due to their metabolic profile are at greater risk of developing GDM and its long-term sequelae. This study aimed to investigate the prevalence and associations of GDM at St George’s Hospital, with emphasis on women diagnosed with PCOS. The K2 maternal med...

ea0034p318 | Reproduction | SFEBES2014

Plant molecules quercetin and resveratrol can affect ovarian cells and invert FSH action

Stochmalova Aneta , Kadasi Attila , Alexa Richard , Sirotkin Alexander

Quercetin and resveratrol, powerful antioxidants and blockers of intracellular mTOR signalling system, are widely distributed in edible plants. Their action on ovarian cell functions and the interrelationships with gonadotropin are studied insufficiently. We examined the effect of FSH, quercetin, resveratrol and their combinations on proliferation and apoptosis of cultured porcine ovarian granulosa cells. Accumulation of marker of proliferation (PCNA) and marker of apoptosis (...

ea0034p319 | Reproduction | SFEBES2014

Fetal glucocorticoid overexposure impacts on germline epigenetic reprogramming in the rat

Rose Catherine M , van den Driesche Sander , Boyle Ashley K , Chambers Thomas , Sharpe Richard M , Meehan Richard R , Drake Amanda J

Background: Fetal glucocorticoid overexposure is associated with low birthweight and increased cardiovascular disease risk in the offspring. Such ‘programmed effects’ can be transmitted across generations through both male and female lines. Disruption of a germline epigenetic reprogramming pathway, characterised by genome-wide erasure and subsequent re-establishment of DNA methylation, may underpin the intergenerational transmission of programmed effects. We used a r...

ea0034p320 | Reproduction | SFEBES2014

Post-endocytic sorting of the LH receptor is mediated by a novel APPL1 dependent mechanism

Sposini Silvia , Jean-Alphonse Frederic , Newcombe Ashley , Hanyaloglu Aylin

LH receptor (LHR) is a G protein-coupled receptor (GPCR) that plays key roles in reproduction and pregnancy. Endocytic trafficking and sorting of GPCRs to diverse cellular fates represent a key mechanism in defining cellular responses by controlling both the temporal and spatial parameters of cellular signalling. How different processes within the endocytic system are coordinated, however, needs to be mechanistically dissected in more detail. We have recently demonstrated that...

ea0034p321 | Reproduction | SFEBES2014

Fertility and pregnancy outcomes for patients with polycystic ovary syndrome in the UK: a retrospective observational study

Morgan Christopher , Jenkins-Jones Sara , Rees Aled

Background: Polycystic ovary syndrome (PCOS) is characterised by anovulation and insulin resistance but the effects on reproductive outcomes are unclear.Objectives: To determine the impact of PCOS upon fertility, pregnancy complications and delivery method.Methods: Data were extracted from the Clinical Practice Research Datalink. Patients with a diagnosis of PCOS (2000–2012) were matched to controls (1:2) by age (±1 year)...

ea0034p322 | Reproduction | SFEBES2014

In the mouse ovary AMH expression is independent of androgen physiology

Lebbe Marie , Woodruff Teresa

Background: Anti-Müllerian hormone (AMH) is a key regulator of preantral follicle development. In human polycystic ovarian syndrome (PCOS) hyperandrogenism drives antral follicle excess, and is associated with elevated AMH levels. It is currently unknown if androgens regulate AMH secretion.Objective and hypothesis: To provide insights into the regulation of AMH action, we hypothesized that dihydrotestosterone (DHT), the most potent androgen, stimula...

ea0034p323 | Reproduction | SFEBES2014

Interaction of androgen with IGF signalling in preantral follicle development in the mouse ovary

Jamall Hina , Laird Mhairi , Hardy Kate , Franks Stephen

Polycystic ovary syndrome (PCOS) is the commonest endocrine disorder in women of reproductive age. It is characterised by excessive ovarian androgen production which, in turn, has been implicated in the aetiology of aberrant follicular development. We have previously reported that prenatal exposure to androgens activates follicle growth1. It has been suggested that androgens may interact with the IGFs to promote the activation and growth of follicles. The aim of thi...

ea0034p324 | Reproduction | SFEBES2014

Regulation of implantation by interaction between the IGF receptor (IGF1R) and miR-145

Lees Miranda , Kang Youn-Jung , Forbes Karen , Aplin John

Successful implantation requires the synchronisation of viable embryonic development with endometrial receptivity. The mechanisms allowing for the initiation of crosstalk remain elusive, however recent studies have revealed alterations in endometrial microRNAs (miRs) in women suffering repeated implantation failure (RIF). We hypothesised that the IGF1 receptor (IGF1R) is involved in implantation, and that miR145, which is elevated in RIF endometrium and predicted to target IGF...

ea0034p325 | Reproduction | SFEBES2014

Renal anomalies in Kallmann syndrome, an uncommon association

Chiran Preeti

We present the case of an 18-year-old boy with phimosis who was referred by the urology team as he had low levels of testosterone. Clinical evaluation revealed that he had normal pubertal growth spurt and was 183 cm tall. His sense of smell was impaired and voice was high pitched. Examination revealed no facial hair, scanty pubic and axillary hair. He had bilateral gynaecomastia, phimosis and micropenis (1.0 cm). The testes were small and soft in consistency (right 2.0 and lef...

ea0034p326 | Reproduction | SFEBES2014

miR-145 is associated with placental growth in mice

Jones Lisa , Charnock Jayne , Forbes Karen

The IGF axis is imperative for normal placental development and function. In the human placenta, IGF1R regulates the actions of IGFs and consequently placental growth. Some microRNAs (miRs) are also known to regulate human placental growth. miR-145 is known to exert its effects on human placental growth by targeting IGF1R. Whilst the expression of some components within the IGF axis are similar between mice and humans, murine expression and function of IGF regulatory miRs has ...

ea0034p327 | Reproduction | SFEBES2014

Glial cells missing 1 transactivates the equine chorionic gonadotrophin beta promoter

Cabrera-Sharp Victoria , Read Jordan , Kitscha Phoebe , Geddis Amelie , Cartwright Judith , de Mestre Amanda

Placental chorionic gonadotrophin (CG) hormone secretion, critical for maintenance of early pregnancy, is dependent on differentiation of specialised CG-secreting binucleate trophoblast (horse) and syncytiotrophoblast (human). The most abundant genes expressed by binucleate cells are the α and β subunits of equine CG. We recently showed the transcription factor glial cells missing 1 (GCM1) is rapidly induced during differentiation of binucleate trophoblast and mRNA e...

ea0034p328 | Reproduction | SFEBES2014

Hypoxia induces terminal differentiation of primary trophoblast cells in vitro

Cabrera-Sharp Victoria , Read Jordan , Mukherjee Abir , de Mestre Amanda

Equine chorionic gonadotrophin (eCG) is not only essential for the maintenance of early equine pregnancy but is also used therapeutically for synchronisation of farm and laboratory animals. Currently it is extracted from serum of farmed pregnant mares. We have recently reported that under normoxic conditions, BMP4 treatment of equine trophoblast resulted in a dose, and developmental stage-dependent increase in total number of differentiated binucleate cells that secrete eCG. O...

ea0034p329 | Reproduction | SFEBES2014

A rare cause of infertility

Imran Siddiqi Ahmed , Menon Ravi

Case history: A 29-year-old man presented with infertility. He also complained of difficulty in passing urine. He also complained of low libido and ejaculate was also noted to be watery. He complained of occasional difficulty in maintaining erections. He denied history of trauma, radiation exposure or use of illicit drugs. He had normal pubertal development and secondary sexual features. He had undergone a surgery about 11 years ago in Nigeria for hypospadias. Hypospadias was ...

ea0034p330 | Reproduction | SFEBES2014

Transforming growth factor-β superfamily signalling and its role in the pathogenesis of heavy menstrual bleeding

Maybin Jacqueline , Boswell Lyndsey , Duncan Colin , Critchley Hilary

Introduction: The human endometrium has a remarkable capacity for repeated repair following the inflammation of menstruation. This occurs without scarring or loss of function but mechanisms involved remain undefined. Aberrations in endometrial repair may lead to pathology such as heavy menstrual bleeding (HMB). The transforming growth factor-β superfamily has been implicated in efficient wound repair and has a potential role in menstrual repair. Downstream of TGF-β, ...

ea0034p331 | Reproduction | SFEBES2014

Effect of endometrial thickness on pregnancy outcome after ICSI

Rehman Rehana , Fatima Syeda Sadia , Khan Taseer , Hussain Mehwish , Khan Rakhshaan

Objective: To study association of endometrial thickness measured on ovulation induction (OI) day with cycle outcome after ICSI.Subjects and methods: It was a quasi experimental design carried out in an infertility clinic from July 2011 till June 2012. The treatment comprised of down regulation of ovaries, controlled ovarian stimulation, oocyte pick up, IVF, embryo (blastocysts) transfer followed by confirmation of pregnancy with beta hCG more than 25 mI...

ea0034p332 | Reproduction | SFEBES2014

Regulation of the LH/CG receptor signalling in human endometrium

West Camilla , Hanyaloglu Aylin , Brosens Jan , Lavery Stuart , Panay Nick

It is well established that the interaction between an implanting embryo and the endometrium is essential for successful implantation and there is a variety of locally secreted factors within the uterine environment which underlies this maternal–fetal cross-talk. Human chorionic gonadotrophin (hCG) is a glycoprotein hormone secreted by the embryo and is essential during early stages of pregnancy to maintain progesterone production from the corpus luteum. Its receptor (LH/...

ea0034p333 | Reproduction | SFEBES2014

Steroid hormones regulate cyclical expression of osteopontin and CD44 in the ovine endometrium

Tremaine Tina , Khalid Mohammed , Wathes Claire , Fouladi-Nashta Ali

Successful embryo implantation requires changes in endometrial gene expression of biomarkers which contribute to uterine receptivity to facilitate blastocyst attachment. Osteopontin (OPN), expressed at the maternal–foetal interface may facilitate implantation in processes, such as cell adhesion, migration and angiogenesis which require OPN signalling via CD44 and integrin receptors. As coordinate expression of OPN and receptors is shown to be cycle-dependent and up-regula...

ea0034p334 | Reproduction | SFEBES2014

Oestrogen implant improves symptoms of hypogonadism and lipid profile in transwomen

Kyaw Ye , Espina Maricel , Seal Leighton

Background: Oestrogen replacement therapy is essential in transwomen for their well-being. Various forms of oestrogen therapy are available. Individual responses to such replacement therapy may vary. Hormonal implant is a reliable form of hormone replacement in postmenopausal women. However, their efficacy and safety in transwomen have not been thoroughly examined yet.Methods: 14 transwomen had 100 mg of oestradiol implant inserted subcutaneously in the ...

ea0034p335 | Reproduction | SFEBES2014

Testosterone implant therapy: efficacy and safety in transmen and native men with hypogondism

Kyaw Ye , Espina Maricel , Seal Leighton

Background: Various forms of testosterone replacement therapy has been used in replacement therapy for transmen and men with hypogonadism. However, efficacy and safety of testosterone implant has not been studied yet.Methods: 11 patietns (five transmen and six men with hypogonadism) had 800–1000 mg of testosterone implant inserted subcutaneously in the anterior abdominal wall. All subjects had previously been on other forms of testosterone replaceme...

ea0034p336 | Reproduction | SFEBES2014

Elevation of HRPE773 (ZG16B) expression in amnion at term and in human ectocervical cell lines treated with inflammatory mediators is consistent with a function in innate immunity

Hayhurst Rachel Rachel , Hibbert Nanette , Frew Lorraine , Stock Sarah , Riley Simon , Morley Steven

Introduction: Labour is an inflammatory process involving the innate immune system, premature activation of which, for example by infection, can lead to preterm birth before 37 weeks of gestation with profound pathophysiological consequences for the offspring. The putative antimicrobial protein HRPE773 (ZG16B) expressed in human female reproductive secretory epithelia, has been shown previously to be elevated in human endometrium during the menstrual early secretory phase and ...

ea0034p337 | Reproduction | SFEBES2014

Maternal and cord blood serum IGF1 IGF binding protein-3, in asymmetrically small for gestational age neonates

Gupta Amrit , Singh Nisha , Tiwari Swasti

This study was planned to investigate the relationship between birth weight and IGF1, IGF binding protein-3 (IGFBP3), in neonates with normal growth (appropriate for gestational age: AGA) and retarded growth (small for gestational age: SGA). A total of 200 mothers were recruited, 120 serum samples were maternal and collected between gestational age of 32-38 weeks, 80 were cord blood from SGA and AGA fetus collected soon after delivery. 98 maternal samples and 48 cord blood sam...

ea0034p338 | Reproduction | SFEBES2014

The melanocortin system in the male reproductive axis

Dowejko Monika , Smith Caroline , Getting Stephen , Le Tissier Paul R , Murray Joanne F

Melanocortin receptors (MCS, MC1–MC5) are GPCRs, activated with different affinities by the melanocortin peptides (α-, β-, γ-MSH and ACTH). They are widely distributed throughout the body displaying a multitude of actions however their role in reproductive physiology is unclear. Previously, we have shown a reduction of pituitary hormone content and abnormalities in testes morphology in male MC3 null mice. The aim ...

ea0034p339 | Reproduction | SFEBES2014

Hyperandrogenism secondary to ovarian hyperthecosis masked by concurrent use of an aromatase inhibitor: a case report

Khan Muhammad , Ahluwalia Rupa , Shore Susannah , Waghorn Alison , Vora Jiten

Backgound: We report a case of a 63-year-old postmenopausal female diagnosed with ovarian hyperthecosis masked by concurrent use of an aromatase inhibitor.Following diagnosis of breast cancer in 2009, requiring mastectomy with adjunctive chemotherapy, she was commenced on anastrozole. Later she noted gradual onset of frontal balding and hirsutism. Biochemistry revealed elevated serum levels of testosterone 13.2 nmol/l (range: <1.9 nmol/l) and androst...

ea0034p340 | Reproduction | SFEBES2014

Two rare conditions in one patient: Fragile X and congenital adrenal hyperplasia

Imran Siddiqi Ahmed

Introduction: I present here a patient with congenital adrenal hyperplasia and Fragile X syndrome. The two conditions are rare and not known to be related to each other.Clinical case: A 26-years-old lady was referred to Endocirne clinic with primary amenorrhoea. She complained of hirsutism and some facial acne since 14 years of age. To the best of her recollection her thelarche was around 12 years of age and pubic hair started to appear at 10 years of ag...