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16th European Congress of Endocrinology

ea0035p740 | Obesity | ECE2014

Bariatric surgery in Prader–Willi syndrome

Chng Edwin

Introduction: Obesity-related complications are the main causes of morbidity and mortality in Prader–Willi syndrome (PWS) patients. We report a case of a 17-year-old patient with PWS who had metabolic complications of poorly controlled diabetes mellitus (HbA1c 8.3% on 56 units of insulin daily) and obesity (BMI 33 kg/m2). He underwent laparoscopic assisted duodenal switch but unfortunately died from aspiration pneumonia 3 days after surgery. A literature search...

ea0035p741 | Obesity | ECE2014

Effects of depressive symptoms on clinical outcomes, inflammatory markers and quality of life after a significant weight loss in a bariatric surgery sample

Frances Carla , Nicolau Joana , Ayala Luisa , Julian Javier , Speranskaya Aleksandra , Zubillaga Ivana , Rivera Rosmeri , Fortuny Regina , Masmiquel Lluis

Background and aims: Obesity is linked to a low-grade and chronic systemic inflammation that improves after a significant weight loss. In the same way, depressive disorder has been suggested to be associated with systemic inflammation up regulation. We aimed to explore whether, after a significant weight loss, the presence of depressive symptoms was associated with differences in terms of inflammatory markers and quality of life compared with individuals without significant de...

ea0035p742 | Obesity | ECE2014

Clinical associations of perceived well-being in obese subjects: a focus on laboratory measurements

Agbaht Kemal , Erim Burcu Rahsan , Karasu Umut

Aims: To document clinical associations of perceived well-being (PWB) in obese subjectsMethods: Prospectively collected ‘Obesity Polyclinic’ database was retrospectively analyzed for the answers of the questionnaries fullfilled during the initial evaluation for obesity. After exclusion unavailable cases, the answers to the question ‘how do you describe your general health/well-being? i) very well ii) well iii) <e...

ea0035p743 | Obesity | ECE2014

Profile of patients treated in our ‘eating disorders unit' from 1996 to the present

Bolivar Victoria Contreras , Raya Rida Nagib , Guerrero Maria Jose Tapia , Romero Maria Fontalba , Lopez Marta Dominguez , Garcia-Salazar Rosario Pilar Fernandez , Serrano David Palao , Romero Antonio Omiste , Fuster Gabriel Olveira , Escofet Federico Soriguer

Rationale: Our aim has been to describe the assistance demand and the profile of patients treated in the eating disorders unit in our hospital.Methods: Observational retrospective study (reviewing medical histories).Results: One thousand two hundred and fourteen patients have been treated at the eating disorders unit between 1996 and 2012, 91.8% female. Age at first visit: 22.1±8.3 years. Time of monitoring tracking: 1.2±...

ea0035p744 | Obesity | ECE2014

The mRNA levels of TNFα, Omentin-1 and PPARγ in adipose tissue of patients with abdominal obesity

Nikolaev Mikhail , Usenka Tatiana , Bazhenova Elena , Zhu Xe , Listopad Olga , Neimark Alexandr , Bogdanov Petr , Berkovich Olga , Baranova Elena , Pchelina Sofya

Adipose tissue is a complex, essential, and highly active metabolic and endocrine organ that release a large numbers of bioactive mediators (adipokines). Obesity is defined as an excessive growth of adipose tissue. As such, it is likely that adipokines could play an important role in the development of diseases associated with obesity including insulin resistance, inflammation, hypertension, cardiovascular risk and metabolic disorders. PPARγ is a ligand-activated transcri...

ea0035p745 | Obesity | ECE2014

The efficacy and safety of 12 weeks Anethum graveolens on waist circumference and triglyceride in patients with metabolic syndrome

Mansouri Masoumeh , Hassani-Ranjbar Shirin , Naebi Neda , Keshtkar Abasali , Larijani Bagher

Introduction: High waist circumference and triglyceride (TG) disturbances are two important and diagnostic components constituting the metabolic syndrome. This study was designed to investigate whether herbal medicine, Anethum graveolens (dill) extract, is capable to improve abdominal obesity presented by high waist circumference and triglyceride in patients with metabolic syndrome.Design: A total of 24 mens and womens who met the criteria of metabolic s...

ea0035p746 | Obesity | ECE2014

Modification in the expression of peripheral appetite signals during hypoxia exposure contributes to anorexia

Duraisamy Arul Joseph , Bayen Susovon , Saini Supriya , Sharma Alpesh Kumar , Vats Praveen , Singh Shashi Bala

Introduction: Anorexia, one of the major problems at high altitude caused by alterations in adipose, gut and pancreatic hormones responsible for appetite regulation and energy balance may be alleviated by understanding the changes during hypoxia. The present study was aimed to elucidate the response of peripheral tissues to hypobaric hypoxic exposure in terms of circulating levels and expression pattern of appetite regulatory hormones, adipose cell morphology and glucose regul...

ea0035p747 | Obesity | ECE2014

Accumulation of abdominal fat in relation to selected proinflammatory cytokines concentrations in non obese Wrocław inhabitants

Bednarek-Tupikowska Grazyna , Zdrojowy-Welna Aleksandra , Stachowska Barbara , Kuliczkowska-Plaksej Justyna , Matczak-Giemza Magdalena , Kubicka Eliza , Tworowska-Bardzinska Urszula , Milewicz Andrzej , Bolanowski Marek

Introduction: Metabolically obese normal weight (MONW) subjects, despite their normal BMI, present metabolic disturbances characteristic of abdominal obesity. One of the reasons might be subclinical inflammation caused by the fat tissue excess. The aim of the study was to asses the association between accumulation of fat (especially abdominal) and concentration of selected proinflammatory cytokines – interleukins (IL-6, IL-18) and C-reactive protein (CRP).<p class="ab...

ea0035p748 | Obesity | ECE2014

Bariatric surgery and bone loss: novel mechanisms and comparison of different modalities

Brzozowska Malgorzata , Bliuc Dana , Hong Angel , Jorgensen John , Talbot Michael , Rigas Georgia , Chen Weiwen , Pocock Nicholas , Eisman John , White Christopher , Baldock Paul , Center Jacqueline

Although bariatric surgery is the most effective weight loss therapy, its skeletal consequences are unclear. The aim of this study was to assess the impact of weight loss, gut hormones, adiponectin on bone loss in people undergoing Dieting (Diet), Gastric Banding (GB) and Gastric Sleeve (GS) over 24 months post-intervention.There were 15 Diet, 8 GB and 20 GS subjects with mean (±S.D.) age 53 (12) years and BMI 39 (6). At 12 months mea...

ea0035p749 | Obesity | ECE2014

The effects of octreotide acetate long-acting repeatable (LAR) on mean platelet volume in acromegaly: octreotidelar may have a detrimental effect on MPV, a new indicator of atherosclerosis

Popovic Djordje , Stokic Edita , Tomic-Naglic Dragana , Zivanovic Zeljko , Bajkin Ivana , Benc Damir , Vukovic Bojan , Kovacev-Zavisic Branka

Introduction and objective: Cardiovascular mortality is high in acromegaly. Our aim in this study was to determine the levels of the new cardiovascular marker in active acromegaly patients, the mean platelet volume (MPV) values before and after octreotide acetate LAR treatment.Material and methods: Thirty-six patients with active acromegaly who presented at the endocrinology department (mean age 46.0±14.0 years and mean BMI: 30.4±5.1 kg/m2...

ea0035p750 | Obesity | ECE2014

Leptin evolution in Aves

Yosefi Sara , Shinder Dmitry , Hen Gideon , Shirak Andre , Serussi Eyal

The discovery of leptin in 1994 has opened a new era in the study of energy balance control at the molecular level. By informing the brain and other tissues the state of fat stores, leptin plays a key role in the control circuits of both appetite and energy expenditure, thus affecting most if not all of the body’s activities. This discovery in mammals stimulated a great interest in the physiological role and molecular mechanism of leptin in chickens. However, a true chick...

ea0035p751 | Obesity | ECE2014

Level of knowledge about type 1 diabetes mellitus among nurses employed at endocrinological dispensaries

Woo Suk

Background: Osteocalcin, a marker of bone formation, is also known as a regulator of glucose and fat mass. The purpose of this study was to determine the association between obesity, metabolic risks and serum osteocalcin in postmenopausal women.Methods: We selected 214 postmenopausal women and determined serum osteocalcin, fasting plasma glucose (FPG), fasting insulin, high-sensitivity C-reactive protein (hs-CRP), the homeostasis model assessment of insu...

ea0035p752 | Obesity | ECE2014

Adiponectin upregulates SHBG production: molecular mechanisms and potential implications

Simo Rafael , Lopez Cristina Saez , Lecube Albert , Hernandez Cristina , Fort Jose Manuel , Selva David M

Epidemiological studies have shown that plasma SHBG levels correlate with plasma adiponectin levels, both in men and women. To the best of our knowledge there are no reports describing any molecular mechanism by which adiponectin regulates hepatic SHBG production. The aim of the present study is to explore whether adiponectin regulates SHBG production by increasing HNF-4α levels through reducing hepatic lipid content. For this purpose, in vitro studies using huma...

ea0035p753 | Obesity | ECE2014

Correlates of visceral and subcutaneous fat thickness in non-diabetic obese and morbidly obese patients

Gul Ozen Oz , Pekgoz Murat , Gullulu Sumeyye , Cander Soner , Tutuncu Ahmet , Seker Ismail , Sag Saim , Acikgoz Ebru , Sarandol Emre , Erturk Erdinc , Tuncel Ercan , Ersoy Canan

Objective: To determine the correlates of visceral and subcutaneous fat thickness in non-diabetic obese and morbidly obese patients.Methods: A total of 31 obese female outpatients composed of morbidly obese (n=16, BMI of ≧40 kg/m2) and obese (n=15, BMI of 30–39.9 kg/m2) patients were included in the present study. Data on age, anthropometrics, blood biochemistry, HOMA–IR, carotid intima-media thick...

ea0035p754 | Obesity | ECE2014

Meaningful and sustained weight loss and improvement of lipid profile in hypogonadal men on long-term treatment with testosterone undecanoate (TU) injections are independent of age: observational data from two registry studies

Saad Farid , Haider Ahmad , Yassin Aksam , Doros Gheorghe , Traish Abdulmaged

Introduction: Improvements of anthropometric and metabolic parameters on long-term testosterone replacement therapy (TRT) from our registry studies have been reported in 2013 (Saad, Obes; Yassin and Doros, Clin Obes; Traish, Int J Clin Pract).Methods: Five hundred and sixty-one hypogonadal men from both registry studies were divided into age groups ≤65 (Group A, n=450) and >65 years (Group B, n=111). All men were treated with ...

ea0035p755 | Obesity | ECE2014

Anthropometric and metabolic parameters in 46 hypogonadal men with obesity grade III improve upon long-term treatment with testosterone undecanoate (TU) injections: Observational data from two registry studies

Saad Farid , Haider Ahmad , Yassin Aksam , Doros Gheorghe , Traish Abdulmaged

Introduction: Numerous studies have reported inverse associations between testosterone and obesity as well as other components of the metabolic syndrome.Methods: From two registry studies of 561 hypogonadal mens, 46 mens with obesity grade III (BMI ≧40 kg/m2) were selected. All patients received TU injections for up to 6 years. 46 mens were followed for 2 years, 43 for 3 years, 37 for 4 years, 34 for 5 years, and 24 for 6 s. Declining n...

ea0035p756 | Obesity | ECE2014

Unstandardized meal does not affect plasma concentrations of leptin, acylated and des-acylated ghrelin in humans

Wosik Hanna , Fryczak Jolanta , Melen-Mucha Gabriela

Background: Leptin and both forms of ghrelin (acylated and des-acylated) are peptide hormones involved in appetite control and food intake regulation in humans. It has been thought that circulating ghrelin concentrations are elevated by fasting and suppressed following a meal, whereas leptin concentrations change in the opposite direction. Most studies, however, only examined these changes after a standardized meal and at different points in time.Objecti...

ea0035p757 | Obesity | ECE2014

Circulating endocannabinoids are associated with cardiometabolic impairment independently of adiposity in normal weight subjects

Fanelli Flaminia , Belluomo Ilaria , Cuomo Gaia , Mezzullo Marco , Baccini Margherita , Casadio Elena , Pasquali Renato , Pagotto Uberto

Obesity is characterized by hyperactivated endocannabinoid System (ECS) in brain and periphery and by increased levels of circulating endocannabinoids (cECs), anandamide (AEA) and 2-arachidonoylglycerol (2AG). It is still debated whether in absence of obesity cECs are only modulated by adiposity or if they are associated with cardiometabolic parameters.We recruited 155 healthy normal weight (NW, BMI: 18.5–24.9 kg/m2) drug-free volunteers ...

ea0035p758 | Obesity | ECE2014

Irisin plasma concentration in PCOS and healthy subjects is related to body adipose content

Pukajlo Katarzyna , Kolackov Katarzyna , Laczmanski Lukasz , Kuliczkowska-Plaksej Justyna , Lenarcik-Kabza Agnieszka , Milewicz Andrzej , Daroszewski Jacek

Polycystic ovary syndrome (PCOS) is a common endocrine abnormality connected not only with reproductive problems but also with impaired energy homeostasis leading to metabolic disorders including metabolic syndrome. Irisin (Ir), recently identified novel myokine, has been proposed to mediate some of the positive influence on metabolic disorders. The aim of our study was to search the relationship between Ir plasma concentration in PCOS in the context of metabolic disturbances....

ea0035p759 | Obesity | ECE2014

May the FTO gene affect visceral obesity? Study of variant rs9930506 SNP among the MONW in a Polish population

Kolackov Katarzyna , Laczmanski Lukasz , Tupikowska Malgorzata , Zdrojowy-Welna Aleksandra , Bednarek-Tupikowska Grazyna

Objective: Genetic factors play a major role in obesity. Numerous studies have confirmed associations of the SNP rs9930506 of FTO gene with increased BMI, hip circumference and total body weight. The metabolic consequences of obesity depend, to a large degree, on body fat distribution.MONW individuals (metabolically obese normal-weight), despite their normal BMI, are characterized by visceral obesity and display metabolic properties that may pre...

ea0035p760 | Obesity | ECE2014

Worsening of cardiovascular risk with increasing number of components of metabolic syndrome in obese non-diabetic individuals

Wolffenbuttel Bruce , Slagter Sandra , Beek Andre Van , Lutgers Helen , Vliet-Ostaptchouk Jana Van , Klauw Melanie Van der

Introduction: The metabolic syndrome (MetS) is a strong predictor of cardiovascular risk. It is influenced by environmental factors, and its prevalence is high in subjects with obesity.Design: We assessed differences in glucose- and lipid parameters, and hsCRP and microalbuminuria (MA) in obese subjects with and without metabolic syndrome (MetS) in LifeLines, a population-based study. Included were subjects 18–80 years, with BMI>30 kg/m2</s...

ea0035p761 | Obesity | ECE2014

Health-related quality of life relates to obesity and low-grade inflammation in obese individuals

Slagter Sandra , Wolffenbuttel Bruce , Beek Andre Van , Lutgers Helen , Vliet-Ostaptchouk Jana Van , Klauw Melanie Van der

Introduction: Obesity is increasingly prevalent in Western society. In addition to medical consequences, obesity also affects health-related quality of life (HR-QOL).Design: We assessed the differences in HR-QOL in obese participants with and without metabolic syndrome (MetS), by level of obesity and level of inflammation, in the LifeLines Cohort study, a population-based study. In total, 12 765 subjects age 18–80 years, with BMI >30 kg/m2<...

ea0035p762 | Obesity | ECE2014

Higher serum levels of the Wnt-signaling antagonist DKK1 in obese respect to Prader–Willi syndrome

Brunetti Giacomina , Delvecchio Maurizio , Graziano Grugni , Ventura Annamaria , Ciccarelli Maria , Piacente Laura , Colucci Silvia , Cavallo Luciano , Grano Maria , Faienza Maria Felicia

Background: Obesity and in particular visceral adiposity has been related to low bone mineral density (BMD) and greater fracture risk. Subjects with Prader–Willi syndrome (PWS) have lower amount of visceral fat than patients with simple obesity, however can develop osteoporosis. A strong relationship between inhibition of the osteoblast formation and induction of the adipocyte differentiation has been demonstrated. Inhibitors of osteoblastogenesis, such as Dickkopf-1 (DKK...

ea0035p763 | Obesity | ECE2014

Evaluation of the prevalence of obesity in Minsk city

Shepelkevich Alla , Labashova Veranika , Salko Olga , Kholodova Elena , Kuzmenkova Elena

Backgrounds: The rapidly increasing epidemic of obesity is one of the most challenging dilemmas facing endocrinologists all over the world today. The scale of the obesity problem has a number of serious consequences for individuals and for government health systems. The aim of our research was the evaluation of obesity and overweight prevalence in Minsk city population within the framework of the Campaign ‘Obesity As A Risk Factor of Type 2 Diabetes Mellitus’<p c...

ea0035p764 | Obesity | ECE2014

Improvement of type 2 diabetes after gastric banding and gastric bypass: medium-term outcomes

Caldas Ana Rita , Pereira Teresa , Garrido Susana , Freitas Claudia , Amaral Claudia , Carvalho Andre , Silva Isabel , Pichel Fernando , Silva Carla , Silva Antonio Sergio , Nogueira Carlos , Santos Jorge , Marcos Mario , Cardoso Maria Helena

Introduction: Bariatric surgery is associated with improvement and often resolution of type 2 diabetes mellitus (T2DM). Our aim was to compare the effects of gastric banding (GB) and gastric bypass (GBP) in glucose homeostasis in obese patients with T2DM.Methods: From 1995 to 2011, 83 diabetic patients underwent bariatric surgery at our hospital: 37 underwent GB (73% females, mean age 48.3±9.2) and 46 underwent GBP (82.6% females, mean age ...

ea0035p765 | Obesity | ECE2014

GLP-1, insulin and glucose levels during mixed test meal before and after gastric bypass surgery in obese patients

Micic Dragan , Polovina Snezana , Jeremic Danka , Micic Dusan , Sumarac-Dumanovic Mirjana

Introduction: Gastric bypass surgery in obese patients results in weight loss, changes in gastrointestinal and pancreatic hormones and improvement of insulin sensitivity.Methods: GLP-1, insulin and glucose levels were measured after test meal (Fresubin drink a 200 ml; 200 kcal, 15% protein, 30% fat and 55% carbohydrate) before (day 0) and 5, 90 and 180 days after gastric bypass surgery. Glycaemia (mmol/l; glucose oxidase);GLP-1 (Active 7–36) (pM/l; ...

ea0035p766 | Obesity | ECE2014

Vertebral bone marrow fat is increased in obese women: relationship with epicardial fat

Ermetici Federica , Briganti Silvia , Delnevo Alessandra , Cannao Paola , Secchi Francesco , Leo Gianni Di , Bandera Francesco , Dozio Elena , Romanelli Massimiliano Marco Corsi , Sardanelli Francesco , Morricone Lelio , Malavazos Alexis Elias

Introduction: Bone marrow fat is considered a risk factor for osteoporosis and fragility fracture. It is not clear if bone marrow adiposity is increased in obesity and how it relates to other compartments of adipose tissue, in particular visceral adipose tissue (VAT), subcutaneous adipose tissue (SAT), and epicardial adipose tissue (EAT).Aim: To evaluate bone marrow adiposity in obese as compared with normal weight women.Design: Tw...

ea0035p767 | Obesity | ECE2014

Abstract withdrawn....

ea0035p768 | Obesity | ECE2014

Serum orexin-A levels, insulin resistance and sleep problems in metabolic syndrome

Parildar Hulya , Cigerli Ozlem , Ergen Nilay , Tarcin Ozlem , Unal Asli Dogruk , Eroglu Hacer , Guvener Nilgun

Background and aims: Orexins are hypothalamic neuropeptides involved in energy homeostasis and sleep regulation. We aimed to find the relation between serum orexin-A levels, glucose, and insulin levels, depression and sleep problems in patients with metabolic syndrome.Materials and methods: This descriptive, cross-sectional study included 43 patients with metabolic syndrome (patient) and 16 healthy subjects (control, age, and sex matched) who applied to ...

ea0035p769 | Obesity | ECE2014

Relationship of serum TSH with BMI, weight and HOMA-IR in euthyroid obese subjects

Garrido Susana , Caldas Ana Rita , Pereira Teresa , Carvalho Andre , Amaral Claudia , Freitas Claudia , Carvalho Rui , Bravo Fatima , Oliveira Jose Carlos , Cardoso Helena

Introduction: Most obese subjects have a normal thyroid function. Despite that, serum TSH seems to be slightly higher in this population, with a positive correlation with the degree of obesity.Objective: Evaluation of the relationship of serum TSH with BMI, weight, and HOMA-IR in euthyroid obese subjectsMethods: Three hundred obese subjects attending an Obesity Outpatient Clinic and submitted to bariatric surgery between January 20...

ea0035p770 | Obesity | ECE2014

GLP1 analogs as treatment of postprandial hypoglycemia after gastric bypass for morbid obesity

Abrahamsson Niclas , Engstrom Britt Eden , Sundbom Magnus , Karlsson Anders

Introduction: In a fraction of morbidly obese subjects undergoing gastric bypass (GBP), episodes with late postprandial hypoglycemia (PPHG) develop 1–3 years after surgery. The pathogenesis of this phenomenon is not fully understood; meal-induced rapid and exaggerated increases of circulating incretins and insulin appear to be partially responsible. Current treatments include low-carbohydrate diets, inhibition of glucose intestinal uptake, reduction of insulin secretion w...

ea0035p771 | Obesity | ECE2014

Adipocyte fatty acid binding protein (A-FABP) deficiency promotes diet-induced obesity partially via its regulation on adaptive thermogenesis

LingLing Shu , PuiZhi Lee , Mi Zhou , Karen S L Lam , AiMin Xu , Ruby L C Hoo

Introduction: Adipocyte fatty acid binding protein (A-FABP) is a fatty acid chaperone that facilitates the efflux of free fatty acid from cytoplasm into circulation. Previous studies show that A-FABP knockout (KO) mice are more susceptible to diet-induced obesity comparing to wild type (WT) littermates. Here we investigate the underlying mechanism of A-FABP in the regulation of diet-induced obesity.Methods: A-FABP KO mice and their WT littermates were fe...

ea0035p772 | Obesity | ECE2014

Offspring of parents with obesity complex investigations: risk of carbohydrate disturbances and diabetes

Dziura Maria , Basiak Aleksander , Wasikowa Renata Barbara

Aim of the study: To examine offspring of patients with simple obesity. To ascertain, if there are some disturbances in the carbohydrate or lipid metabolism or unknown type 2 diabetes in these subjects.Method and subjects: Examined were 132 families, 108 families with obesity, and 24 families without obesity, the control group. Fourteen additional were excluded because of ascertained at the time of examination unknown type 2 diabetes in the parents. In a...

ea0035p773 | Obesity | ECE2014

Downregulation of complement C3 and C3aR expression in subcutaneous adipose tissue in obese Caucasian women

Gupta Abhishek , Rezvani Reza , Lapointe Marc , Poursharifi Pegah , Marceau Picard , Tiwari Sunita , Tchernof Andre , Cianflone Katherine

Background: The central component of the complement system, C3, is associated with obesity, type 2 diabetes and cardiovascular disease however the underlying reasons are unknown. In the present study we evaluated gene expression of C3, the cleavage product C3a and its cognate receptor C3aR in subcutaneous and omental adipose tissue in women.Methods: Women (n=140, 21–69 years, BMI 19.5–79 kg/m2) were evaluated for anthropomet...

ea0035p774 | Obesity | ECE2014

Stimulation of cystatin C production in adipocytes by insulin, GH and triiodothyronine (T3)

Schmid Christoph , Ghirlanda Claudia , Zwimpfer Cornelia , Schmid Diri , Tschopp Oliver

Objective: Cystatin C (CysC) is a marker for estimation of glomerular filtration rate (GFR) and a predictor of mortality, beyond that provided by GFR. CysC increases with abdominal obesity and insulin resistance independently of GFR, not necessarily due to changes in renal clearance but possibly due to changes in production. Non-GFR determinants of CysC remain poorly defined. GH or thyroid hormone excess (acromegaly and hyperthyroidism) lead to concomitant increases in GFR and...

ea0035p775 | Obesity | ECE2014

Free vs total testosterone as a marker of Leydig cell function and androgen exposure in obese male adolescents

Vandewalle Sara , Taes Youri , Fiers Tom , Maria Van Helvoirt , Patrick Debode , Jean De Schepper , Kaufman Jean-Marc

Background: Data concerning pubertal development and testosterone (T) in obese male adolescents (ObA) are scarce and contrasting. Underperforming T immunoassays and poor assessment of pubertal development might explain discordant findings. Although SHBG is markedly affected, few studies report free T (FT) levels.Objective: To study if ObA have conserved FT levels despite low total T (TT) and to investigate if FT is a better indicator of androgen exposure...

ea0035p776 | Obesity | ECE2014

Application of LC–MS and CE–MS based metabolomics to study type 2 diabetes development in lean, overweight and obese humans

Ciborowski Michal , Kretowski Adam , Adamska Edyta , Citko Anna , Waszczeniuk Magdalena , Wilk Juliusz , Golonko Anna , Pliszka Justyna , Lipinska Danuta , Goscik Joanna , Rusak Magdalena , Godzien Joanna , Barbas Coral , Gorska Maria

The risk of type 2 diabetes mellitus (T2DM) development is related to BMI, therefore this disease mainly occurs among overweight (OW) and obese (OB) people. However lean (L) individuals may also suffer from T2DM. The evolution of T2DM is a multistep process and starts with insulin resistance (IR), which may evolve into pre-diabetic state i.e.: impaired fasting glucose (IFG) and/or impaired glucose tolerance (IGT). In up to 70% of patients pre-diabetic state evolves into T2DM. ...

ea0035p777 | Obesity | ECE2014

The outcome results in patients with type 2 diabetes mellitus and obesity after either adjustable gastric banding (AGB) or Roux-en-Y (RNY) gastric bypass

Rys Andrzej , Dugal Tabinda

The prevalence of the type 2 diabetes and obesity are on the rise globally. Initial interventions for these groups of patients remain diet, exercise and medications. If these measures are insufficient gastrointestinal surgery offers a very good alternative for obesity and type 2 diabetes treatment.We report the outcome results for patients who underwent either adjustable gastric banding (AGB) or Roux-en-Y (RNY) gastric bypass in the years 2009–2012....

ea0035p778 | Obesity | ECE2014

Association analysis of the D2 dopamine receptor gene Taq IA polymorphisms in a cohort of Belarus morbidly obese children and adolescents.

Aksyonova Elena , Seyitnazarova Ayjan , Solntsava Anzhalika , Zagrebaeva Olga , Mikhno Hanna , Sukalo Alexandr

Background: The A1 allele of the DRD2/ANKK1 Taq1A polymorphism (rs1800497) is associated with reduced striatal D2 receptor binding (Thompson et al., 1997; Pohjalainen et al.,1998). Associations between Taq1A polymorphism DRD2/ANKK1 gene and reward sensitivity including compulsive overeating were reported (Propper et al., 2008; Davis et al., 2008). To study the association of DRD2/ANKK1 Taq1A polymorphism (rs1800497) with obesity we started g...

ea0035p779 | Obesity | ECE2014

The adaptor protein APPL2 acts as a central regulator of energy metabolism

Wang Baile , Cheng Kenneth KY , Li Xiaomu , Lam Karen SL , Xu Aimin

Introduction: We have previously demonstrated that APPL1 maintains glucose homeostasis by promoting both actions and secretion of insulin. Although APPL2 is a close homolog of APPL1, the physiological function of APPL2 is vaguely characterized. Thus, we generated a knockout (KO) mouse model in which APPL2 is deleted in pancreatic β-cells and hypothalamus (which is called APPL2 KO mice) to investigate its role in glucose and energy metabolism.Methods...

ea0035p780 | Obesity | ECE2014

Adiposity changes show primacy over the effect of insulin sensitivity changes on androgen profile in obese men integrating short-term weight loss programs

Gama Filipa , Espirito-Santo Raquel , Silva-Nunes Jose , Sabino Teresa , Santos Cristina C , Agapito Ana

Introduction: Several studies point towards lower testosterone levels (TL) in patients with obesity and/or insulin resistance (IR).Aim: To analyze the influence of anthropometric parameters and IR on TL in obese men and weight-dependent changes after a short-term weight loss program.Methods: Data were collected from a group of male patients, aged 18 to 65, when they attended a first obesity appointment and at reassessment 6 months ...

ea0035p781 | Obesity | ECE2014

Hyperleptinemia and insulin resistance in obesity-associated sleep apnoea: Sex-dependent effects of CPAP application

Andrada Patricia , Pascual Eider , Perez Belen , Gutierrez Gala , Pujante Pedro , Silva Camilo , Iriarte Jorge , Escalada Javier , Galofre Juan Carlos , Gomez-Ambrosi Javier , Fruhbeck Gema , Salvador Javier

Obstructive sleep apnoea (OSA) is very common in severe obese patients and has been related to insulin resistance (IR) and hyperleptinemia, and these findings may have effects on food behavior and obesity complications. In order to explore this relationship and the role of sex we have studied 531 patients with morbid obesity (BMI 42.8+6.5 kg/m2). A full polysomnographic study and glucose, insulin and leptin measurements as well as air displacement plethysmography we...

ea0035p782 | Obesity | ECE2014

Use of visceral fat percentage and body fat percentage compared to anthropometric parameters in predicting obesity and cardiovascular risk in Sri Lankan population

Karunasena Nayananjani , Bulugahapitiya Uditha , Chulasiri Pubudu , Ariyawansa Shalika

Introduction: Visceral adiposity is shown to be associated with cardiovascular disease. Aim of this study was to evaluate the usefulness of visceral fat percentage (VF%) and body fat percentage (BF%), compared to anthropometric parameters, BMI waist circumference (WC), and waist – hip ratio(WHR) in predicting obesity and cardiovascular risk.Method: A descriptive cross sectional study conducted within five health divisions in Colombo district in Sri ...

ea0035p783 | Obesity | ECE2014

Decreased expression of genes encoding thermogenesis-related proteins in adipose tissues of obese patients is not associated with their methylation status

Kurylowicz Alina , Jonas Marta , Lisik Wojciech , Jonas Maurycy , Wicik Zofia Agnieszka , Wierzbicki Zbigniew , Chmura Andrzej , Puzianowska-Kuznicka Monika

Introduction: Thermogenesis constitutes an important part of energy expenditure and its disturbances may contribute to the development of obesity. The aim of this study was to investigate whether the expression of thermogenesis-related genes differs in adipose tissues of obese and normal-weight individuals and if methylation of CpG islands located in the regulatory regions of these genes is involved in this phenomenon.Materials and methods: The expressio...

ea0035p784 | Obesity | ECE2014

Different relationship between serum 25OH-vitamin D and expression of vitamin D 25-hydroxylase gene CYP2R1 in subcutaneous adipose tissue of obese and slim subjects.

Jonas Marta , Kurylowicz Alina , Bartoszewicz Zbigniew , Lisik Wojciech , Jonas Maurycy , Wierzbicki Zbigniew , Chmura Andrzej , Puzianowska-Kuznicka Monika

Introduction: In Central Europe, vitamin D deficiency is common, and a very pronounced deficiency of this vitamin is observed in obese individuals. We hypothesized that the decreased serum concentration of vitamin D might be in part due to the changed metabolism of this vitamin in adipose tissues.Methods: We assessed the expression of vitamin D 25-hydroxylase genes CYP2J2 and CYP2R1 in adipose tissues of obese and slim patients using RT...

ea0035p785 | Obesity | ECE2014

Adiponectin gene expression in subcutaneous and deep neck adipose tissue

Jaksic Vlatka Pandzic , Grizelj Danijela , Marusic Maruska , Boscic Drago , Ozegovic Ivan , Vlasic Ana , Ajduk Marko , Pavic Predrag , Subaric Marin , Kusec Rajko , Jaksic Ozren

Adipose tissue is an endocrine organ that actively contributes to the development of the metabolic dysfunction but it is not homogenous and the nature of its impact considerably depends on its anatomic location. So far both subcutaneous and deep neck adipose tissue depots were individually related to increased metabolic risk but their particular features were not mutually compared. Adiponectin is an adipocytokine that has consistently showed higher mRNA expression in subcutane...

ea0035p786 | Obesity | ECE2014

Change in cardiovascular risk factors Following bariatric surgery for obesity – First Sri Lankan experience

Karunasena Nayananjani , Wijeratne Thejana , Bulugahapitiya Uditha , Chulasiri Pubudu , Kasturiaratchi Kaushalya , Abeysinghe Priyanthika , Gunawardana Waruni Abeysiri

Introduction: Obesity is associated with increased prevalence of cardiovascular(CV) risk factors and mortality and the CV risk factors are known to reduce following bariatric surgery. The aim of this analysis was to determine the effect of bariatric surgery on CV risk factors and CV risk score.Method: A prospective analytical study of the first 15 consecutive patients who underwent laparoscopic Sleeve Gastrectomy procedure from 2009 to 2011 in Colombo. B...

ea0035p787 | Obesity | ECE2014

The association between metabolic syndrome and android fat mass and android to gynoid fat mass ratio- are Classification and Regression Trees models helpful?

Brona Anna , Milewicz Andrzej

The aim of the study was to estimate thresholds of android fat mass and android to gynoid fat mass ratio at which metabolic disorders and blood pressure typical of metabolic syndrome appear. Classification and Regression Trees models were used to estimate these thresholds for glucose, HDL cholesterol, triglycerides and systolic and diastolic blood pressure.Methods: healthy postmenopausal women were recruited for the study. Blood samples were collected fo...

ea0035p788 | Obesity | ECE2014

Dietary fat alters the expression of cortistatin and ghrelin systems in the PBMCs of elderly subjects: Putative implications in the postprandial inflammatory response

Gahete Manuel D. , Luque Raul M. , Yubero-Serrano Elena M. , Cruz-Teno Cristina , Ibanez-Costa Alejandro , Delgado-Lista Javier , Gracia-Navarro Francisco , Perez-Jimenez Francisco , Castano Justo P. , Lopez-Miranda Jose

Dietary fat influences systemic inflammatory status, which, in turn, determines the progression of age-associated diseases. Peripheral blood mononuclear cells (PBMCs) comprise a subset of white blood cells placed at the crossroad between diet and inflammation, in that they play a key role in the immune/inflammatory system and their gene expression pattern is influenced by diet. Since the somatostatin (SST), cortistatin (CORT) and ghrelin systems have been shown to modulate the...

ea0035p789 | Obesity | ECE2014

Climate and risk of obesity: study

Valdes Sergio , Maldonado-Araque Cristina , Garcia-Torres Francisca , Goday Albert , Calle-Pascual Alfonso , Castano Luis , Castell Contxa , Catala Miguel , Delgado Elias , Franch Josep , Gaztambide Sonia , Gomis Ramon , Menendez Edelmiro , Ortega Emilio , Vendrell Joan , Soriguer Federico , Rojo-Martinez Gemma

Introduction: It has been suggested that the lack of exposure to low ambient temperatures may be a contributor to the development of obesity. The aim of this study was to study possible associations between climate and the risk for obesity in the Spanish population using an ecological focus.Methods: The Study is a national epidemiological study designed to determine the prevalence of diabetes, obesity and other cardiovascular risk factors in Sp...

ea0035p790 | Obesity | ECE2014

Sex hormone binding globulin, total and HMW adiponectin and insulin resistance among normal weight postmenopausal women with metabolic syndrome

Sieminska Lucyna , Walczak Krzysztof

Visceral obesity among postmenopausal women often leads to the development of metabolic syndrome (MS), but there is a subgroup of normal weight postmenopausal women displaying metabolic disturbances who are diagnosed to have MS. These subjects often show insulin resistance, however little is known about the effects of adiponectin and sex hormone binding globuline SHBG) on MS in this subset of women.The aim of the study was: i) to assess serum SHBG, total...

ea0035p791 | Obesity | ECE2014

Childhood obesity alters innate T cell frequency and function resulting in loss of regulation and increased inflammation

Hogan Andrew , Carolan Eirin , Mangan Bozgana , Corrigan Michelle , Doherty Derek , Cody Declan , O'Shea Donal

Background: Childhood obesity now represents a major public health concern. Being obese in childhood appears to increase the risk of severe obesity in adulthood. Obesity is associated with co-morbid conditions such as type 2 diabetes and cardiovascular disease. Underpinning obesity is a state of chronic sterile inflammation. Recently the invariant natural killer T (iNKT) cell, an innate T cell was shown to act as a metabolic regulator and altered by obesity both in adults and ...

ea0035p792 | Obesity | ECE2014

Metabolic and hypertension control in diabetic patients submitted to bariatric surgery

Fonseca Ricardo , Duarte Joao Sequeira , Serra Filipa , Moniz Catarina , Ferreira Rute , Gouveia Clotilde , Oliveira Manuela , Saraiva Antonio Machado

Background: Type 2 diabetes and Hipertension are common in obese patients. Bariatric surgery is effective in sustained weight loss, but also improves glycemic and hypertension control. A high percentage of diabetes remission occurs.Methods: Retrospective study of type 2 diabetic patients submitted to bariatric surgery between 2006 and 2013 in a central hospital. Primary endpoint was evaluation of diabetes and hypertension remission, 2 years after surgery...