The pathophysiology differs significantly between primary and secondary hyperparathyroidism. Whole exome sequence analysis of parathyroid adenomas showed few somatic variants. The underling mechanisms of the occurrence of sporadic primary hyperparathyroidism remain largely unknown. In this study, we analysed the differences in gene expression between primary and secondary hyperparathyroidism to explore potential functional alterations. Total RNA was extracted from tissues obtained during parathyroidectomy. Gene expression microarray and bioinformatic pathway analysis were performed. Parathyroid tissues were classified in an unsupervised manner into primary and secondary clusters. Parathyroid adenomas showed higher expression of cell adhesion molecules. On the contrary, secondary hyperparathyroidism exhibited upregulation of complement/coagulation cascades and mTOR signalling pathway. In conclusion, our study demonstrates that different pathophysiology led to differential gene profiling in hyperparathyroidism. The results from pathway analysis may have diagnostic and therapeutic implications.
Disclosure: This work was supported by the Ministry of Science and Technology of Taiwan (MOST 103-2314-B-195-015-MY3).