ECE2015 Guided Posters Pituitary–Neuroendocrinology and central salt regulation (9 abstracts)
Insulin-induced hypoglycaemia decreases IGF1 bioactivity in humans: a missing link to increased mortality in diabetic patients?
Zeinab Sarem 1, , Biruhalem Assefa 1, , Martin O Weickert 4, , Aikatarini Adamidou 1 , Jan Frystyk 6 , Matthias Möhlig 1 , Stefanie Lieske 2 , Andreas L Birkenfeld 1, , A.F.H Pfeiffer 1, , Joachim Spranger 1, & Ayman M Arafat 1,
1Department of Endocrinology, Diabetes and Nutrition, Charité-University Medicine Berlin, Berlin, Germany; 2Department of Clinical Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, Germany; 3Department of Endocrinology, Diabetes and Nutrition at the Center for Cardiovascular Research (CCR), Charité-University Medicine Berlin, Berlin, Germany; 4Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism,University Hospitals Coventry and Warwickshire NHS Trust, Coventry, UK; 5Division of Metabolic & vascular Health, Warwick Medical School, University of Warwick, Coventry, UK; 6Medical Research Laboratories, Institute of Clinical Medicine, Faculty of Health Sciences, Aarhus University, DK-8000 Aarhus C, Aahrus, Denmark; 7Department of Endocrinology, Diabetes and Nutrition at the Experimental and Clinical Research Center (ECRC), Charité-University Medicine Berlin and Max-Delbrück Centre Berlin-Buch,, Berlin, Germany; 8Section of Metabolic Vascular Medicine,Medical Clinic III, University Clinic Dresden, Dresden, Germany.
Background: The mechanisms underlying the association between severe hypoglycaemia and increased cardiovascular mortality among patients with diabetes mellitus are not fully understood. Our aim was to evaluate changes in GH/IGF1 axis during insulin-induced hypoglycaemia, as a possible link.
Methods: Twenty-five healthy subjects, 12 obese participants (OP; 6/ 6; 34.4±1.7 kg/m2), and 13 healthy lean participants (LP; 6/ 7; 21.7±0.6 kg/m2) were studied using insulin tolerance test and changes in GH, total IGF1, IGF binding proteins (IGFBPs) and IGF1 bioactivity, measured by the cell-based KIRA method, were investigated. Moreover, the effect of insulin on mRNA expression of parts of the GH/IGF1 system was further studied in mouse primary hepatocytes.
Results: Under hypoglycaemic conditions, insulin significantly increased IGFBP2 and decreased IGF1 bioactivity in both groups (P<0.01). This was followed by a surge in GH (P<0.01). As expected, insulin decreased IGFBP1 levels, whereas no changes in total IGF1 and IGFBP3 levels were observed. In vitro, insulin stimulation of primary hepatocytes lead to a decrease of IGFBP1 and IGFBP3 and increase of IGFBP2 mRNA expression.
Conclusion: The insulin-induced hypoglycaemia is associated with a decrease in IGF1 bioactivity through up-regulation of IGFBP2. Our results point to a possible and previously poorly explored mechanism explaining the strong association between hypoglycaemia and increased cardiovascular mortality among diabetic patients.
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more