Searchable abstracts of presentations at key conferences in endocrinology
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18th European Congress of Endocrinology

Guided Posters


ea0041gp129 | Obesity | ECE2016

Direct effects of dopamine on mitochondrial thermogenesis in brown adipocytes

Kohlie Rose , Perwitz Nina , Lehnert Hendrik , Klein Johannes , Iwen Alexander

Brown adipose tissue (BAT) is key in energy homeostasis. Catecholamines are critically involved in the regulation of BAT-thermogenesis, yet current research has focussed on noradrenaline and adrenaline. Some evidence suggests a role of dopamine (DA) in BAT-thermogenesis but the intracellular mechanisms have not been addressed. We applied our extensively characterised murine brown adipocyte cell line to address these questions.D1-like and D2-like receptor...

ea0041gp130 | Obesity | ECE2016

Hypothalamic inflammation in humans is not reversed by a profound weight loss and an improved insulin sensitivity due to bariatric surgery

Kreutzer Carina , Peters Sonke , Schulte Dominik , Turk Kathrin , Wolff Stephan , Rohr Axel , Kerby Tina , Riedel Christian , van Eimeren Thilo , Schreiber Stefan , Laudes Matthias

Introduction: Obesity is associated with hypothalamic inflammation (HI) in animal models. While MRI studies in humans also found an increased intensity in the MBH in obese subjects, it remains unclear (1) if HI causes neuronal death and (2) if HI reverses during weight loss.Patients and methods: n=50 obese subjects and n=50 age- and sex-matched controls where examined. MRI scans including spectroscopy were performed. Also, detailed nutr...

ea0041gp131 | Obesity | ECE2016

The liver of obese patients with hepatic steatosis exhibits a severe dysregulation of key splicing machinery components as compared to obese patients without hepatic steatosis

Rio-Moreno Mercedes del , Pedraza-Arevalo Sergio , Gonzalez-Rubio Sandra , Ferrin Gustavo , Gahete Manuel D , Rodriguez-Peralvarez Manuel , Kineman Rhonda D , Ibanez-Costa Alejandro , de la Mata Manuel , Castano Justo P , Luque Raul M

Obesity, a disease that is growing at epidemic proportions worldwide, is caused by a combination of genetic and lifestyle factors. One of the most common pathologies associated with obesity is hepatic steatosis, an accumulation of fat within the liver that can progress to liver fibrosis, cirrhosis, and ultimately lead to hepatocellular carcinoma. There is emerging evidence that alternative mRNA splicing, the key mechanism providing transcript and protein diversity, is dysregul...

ea0041gp132 | Obesity | ECE2016

The E3 ubiquitin ligase MDM2 acts as a key determinant of hepatic VLDL-triglycerides and ketone body production in obesity

Liu Zhuohao , Xu Aimin , Cheng Kenneth K Y

Introduction: Obesity is a major risk factor for the development of hyperlipidemia and its related cardiovascular complications. Apart from its well-established role in cancer biology, the MDM2-p53 axis has been recently shown to regulate glucose and lipid metabolism. Our preliminary data indicated that MDM2 is dramatically induced in the liver of obese mice. In this study, we aimed to investigate the potential role of hepatic MDM2 in controlling systemic lipid homeostasis usi...

ea0041gp133 | Obesity | ECE2016

The ubiquitin-like pathway neddylation controls adipocyte differentiation, obesity and metabolism

Bauder Corinna , Labeur Marta , Vogl Annette , Wolfel Barbara , Wurst Wolfgang , Stalla Gunter , Refojo Damian

The ubiquitin-proteasome cascade has been described as a critical factor in adipocyte biology, insulin signaling and obesity, but the function of Nedd8, the ubiquitin-like protein with the closest homology to ubiquitin, remains unknown. Using 3T3-L1 preadipocyte cells, we found that MLN-4924, a specific neddylation inhibitor, blocks adipocyte differentiation. Screening for signaling and transcriptional factors driving adipogenesis, we found that neddylation blockade inhibits t...

ea0041gp134 | Obesity | ECE2016

Prognostic factors and pathophysiology of diabetes remission after metabolic gastric bypass, sleeve gastrectomy and greater curvature plication: a randomized controlled trial

Vilarrasa Nuria , Casajoana Anna , de Gordejuela Amador R , Duran Xavier , Vendrell Joan , Fernandez Sonia , Garrido Pilar , Monseny Rosa , Pujol Jordi

Introduction: 39% of morbidly obese patients have type 2 diabetes mellitus (T2DM) and 80% of them can achieve diabetes remission after bariatric surgery. However, there are few randomized studies comparing the metabolic results of different surgical techniques and the hormonal mechanisms involved.Objective: To study and compare the improvement of T2DM and the hormonal pathways following three surgical techniques: metabolic gastric bypass (mGBP), sleeve g...

ea0041gp135 | Obesity | ECE2016

Ventilatory anaerobic threshold six months after RYGB

Polovina Snezana , Nedeljekovic Ivana , Bajec Djordje , Sumarac-Dumanovic Mirjana , Radenkovic Dejan , Kendereški Aleksandra , Gregoric Pavle , Cvijovic Goran , Micic Dusan , Zoric Svetlana , Stamenkovic-Pejkovic Danica , Milin-Lazovic Jelena , Jeremic Danka , Gligic Ana , Micic Dragan

Introduction: Obesity is associated with high risk for coronary artery disease, high blood pressure, elevated serum cholesterol level, malignancy and Type 2 diabetes mellitus. After weight loss, especially after bariatric surgery there is improvement in blood pressure, metabolic parameters and ventilatory anaerobic threshold.Material and methods: Ergospirometry (Shiller CS-200, Bruce protocol) was performed in 50 obese patients before and six months afte...

ea0041gp136 | Obesity | ECE2016

A novel human fetal brown stem cell functional model to study brown adipogenesis

Franco Alessandra Di , Guasti Daniele , Squecco Roberta , Mazzanti Benedetta , Rossi Francesca , Idrizaj Eglantina , Gallego-Escuredo Jose , Villarroya Francesc , Bani Daniele , Forti Gianni , Vannelli Gabriella Barbara , Luconi Michaela

The potential therapeutic applications of targeting brown adipose tissue open new clinical avenues in fighting against metabolic pathologies. However, due to the limited brown depots in adult humans, dramatically reduced after birth, solid cell models to study human brown adipogenesis and its pathophysiological regulation are urgently needed.In our study, we characterized a novel human model of brown adipose stem cells, hfB-ASC, derived from fetal inters...

ea0041gp137 | Obesity | ECE2016

Adipose tissue infiltration in normal-weight subjects and its impact on metabolic function

Moreno-Indias Isabel , Oliva-Olivera Wilfredo , Castellano-Castillo Daniel , Lhamyani Said , Fernandez-Garcia Jose Carlos , Queipo-Ortuno Maria Isabel , Cardona-Diaz Fernando , Tinahones Francisco

Objectives: To study for the first time the differences in monocyte/macrophage infiltration, inflammation and adipogenesis in the subcutaneous and visceral adipose tissues of normal-weight subjects who differed in their degree of metabolic syndrome.Methods: The study included 92 normal-weight subjects who differed in their degree of metabolic syndrome. Anthropometric and biochemical parameters were measured. RNA from subcutaneous and visceral adipose tis...

ea0041gp138 | Obesity | ECE2016

Dietary and weight loss effects on human gut microbiome diversity and metabolism

Fangmann Daniela , Heinsen Femke-Anouska , Schulte Dominik M , Ruhlemann Malte-Christoph , Turk Kathrin , Settgast Ute , Muller Nike , Lieb Wolfgang , Baines John F , Schreiber Stefan , Franke Andre , Laudes Matthias

Introduction: Changes in the gut microbiome have been associated with the development of obesity. The aim of the present study was to examine i) the effect of a formula based very-low-calorie weight loss diet (VLCD) on the gut microbiome of obese humans and ii) whether if potential changes are sustained during weight maintenance.Patients and methods: The study consisted of 3 months VLCD (~800 kcal/d) followed by 3 months of weight maintenance. 18 obese h...