Endocrine Abstracts

Introduction: Endocrine disruptor compounds (EDCs) are chemicals that may interfere with the endocrine system and produce adverse developmental, reproductive, neurological and immune effects.These agents can be found in household, in the industry and in our environment.Endocrine disruptors are substances that interfere with the synthesis, secretion, transport, binding, action, or elimination of natural hormones in the body that are responsible for development, behaviour, fertility, and maintenance of homeostasis.Our aim was to investigate the effects of uron herbicides EDCs/fenuron (PU), monuron (MU), diuron (DU)/on the normal endocrine regulation; particularly the monoamine activated arginine-vasopressine(AVP)release from neurohypophyseal cells.

Materials and methods: The primary monolayer cell cultures were the neuroendocrine regulated models. These were prepared from the pituitary of normal Wistar rats (♂).The separated neurohypophysis (NH) tissues were dissociated by enzymatic (trypsine, collagenase, DNA-se I; II) and mechanic methods. The 14 days old cultures were standardized for cell-viability and AVP content in NH cultures. The NH cells were tested for functionally AVP hormone volume by aspecific osmotic stimulus (30 mM K⁺). Untreated cultures were used as controls while further cultures were treated with:- I.: 1 hour 0,1 μg/ml EDCs: MU, DU, PU -II.: 10⁻⁶M monoamines (norepinephrine, dopamine, histamine) alone -III.: the monoamines agents with EDCs combined. The AVP release was measured from supernatant media by RIA method.

Results: The uron herbicides alone did not have significant effects on the AVP release in the NH cell cultures. The monoamine activated hormone release strongly changed by the EDCs, mainly the dopamine and histamine.

Conclusion: The psycho-neuro-endocrine-immune system modulated by various environmental factors can lead to the development of different diseases in connection with this complex network. This work was supported by: TÁMOP-4.2.2.D-15/1/KONV-2015-0010, TÁMOP-4.2.4.A/2-11-1-2012-0001