Searchable abstracts of presentations at key conferences in endocrinology
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Society for Endocrinology BES 2016

Brighton, UK
07 Nov 2016 - 09 Nov 2016

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07 Nov 2016 to 09 Nov 2016 Brighton, UK Further information

Poster Presentations


ea0044p200 | Reproduction | SFEBES2016

Defining uterine insulin resistance

Shao Ruijin , Zhang Yuehui , Hu Min , Billig Hakan

Peripheral insulin resistance and hyperandrogenism are the primary features of polycystic ovary syndrome (PCOS). However, how insulin resistance and hyperandrogenism affect uterine function and contribute to the pathogenesis of PCOS are open questions. Rodent models of insulin and human chorionic gonadotropin (hCG) treatment have provided systems in which to study ovarian insulin resistance, ovarian stromal hyperplasia, follicular cyst formation, and impaired mitochondrial fun...

ea0044p201 | Reproduction | SFEBES2016

Alterations of specific caveolin isoforms in the rat uterus under insulin resistance and hyperandrogenism conditions: does metformin contributes to their regulation?

Hu Min , Zhang Yuehui , Sun Xue , Sun Xiaoyan , Meng Fanci , Shao Ruijin , Billig Hakan

The caveolin (CAV) consists of three isoforms (CAV1, CAV2, and CAV3) and contributes to insulin-regulated glucose metabolism. It has been reported that decreased caveolin-1 phosphorylation in the endometrium is linked with insulin resistant state in women with polycystic ovary syndrome (PCOS). Because PCOS patients often display hyperandrogenism, whether hyperandrogenism in addition to insulin resistant is involved in the regulation of caveolin expression and activation in the...

ea0044p202 | Reproduction | SFEBES2016

Understanding and supporting women with polycystic ovary syndrome: a qualitative study in an ethnically diverse UK sample

Hadjiconstantinou Michelle , Mani Hamidreza , Patel Naina , Levy Miles , Davies Melanie , Khunti Kamlesh , Stone Margaret

Background and aims: Polycystic Ovary syndrome (PCOS) is a common, lifelong condition. Its associated symptoms have been linked with psychological consequences, but less attention has been given to the daily implications of living with the condition. We aimed to explore women’s experiences of living with PCOS, including a specific focus on practical implications and methods of providing support such as structured group-based patient education.Method...

ea0044p203 | Reproduction | SFEBES2016

Elicitation of estrogenic and antiandrogenic mechanisms by oleic acid in pubertal male rats

Oyelowo Oluwakemi , Bolarinwa Adeyombo

This study was designed to determine if prenatal exposure to oleic acid would alter testicular endocrine functions in either an estrogenic or antiandrogenic manner at puberty. Gravid dams were distributed into four groups of five rats each as follows: Control group (1 ml/kg olive oil throughout pregnancy), pre-treatment group (1000 mg/kg of oleic acid for 7 days before mating), preimplantation group (1000 mg/kg of oleic acid for the period of preimplantation), Organogenesis gr...

ea0044p204 | Reproduction | SFEBES2016

Gonadotropin Induction of Spermatogenesis in Men with Hypogonadotrophic Hypogonadism: an audit

Jinadev Pushpa , Ahern Tomas , Pichaipillai Lakshmi , Tofeec Khaled , Gibson Christine , Komlosy Nicci , Wu Frederick

Introduction: Infertility affects 15% of couples worldwide. Male factors account for half of cases seeking medical care.Objectives and methods: To ascertain whether the American Association of Clinical Endocrinologists (AACE) guidelines regarding gonadotrophin induction of spermatogenesis in men with hypogonadism are being followed in our unit. Data were collected retrospectively from clinical records.Results: 26 couples were ident...

ea0044p205 | Reproduction | SFEBES2016

An audit of success rates of the induction of spermatogenesis clinic at University College London Hospitals

Dimakopoulou Anastasia , Ojewuyi Taofeek , Llahana Sofia , Baldeweg Stephanie , Conway Gerard

Introduction and aim: In the UK, 15% of couples are affected by infertility which is due to primary or secondary male factors in 30%. Induction of spermatogenesis with gonadotrophins is a long established endocrine treatment for gonadotrophin deficiency. However, there are concerns that some patient groups have very low success rates. The aim of our audit was to define success of spermatogenesis and fertility rates amongst these men.Methods: We retrospec...

ea0044p206 | Reproduction | SFEBES2016

The effects of Vitamin D on the outcomes of controlled ovarian stimulation in women with and without Polycystic Ovary Syndrome undergoing in vitro fertilisation

Cunningham Thomas , Allgar Victoria , Atkin Stephen , Kilpatrick Eric , Maguiness Stephen , Sathyapalan Thozahukat

Background: Vitamin D deficiency has become the most common nutritional deficiency throughout the world. 67–85% of Polycystic Ovary Syndrome (PCOS) patients have low serum levels of Vitamin D. Studies have reported conflicting data as to whether or not Vitamin D may play a role in human reproduction. The objective of this prospective cohort study was to investigate what effect vitamin D had on parameters within an in vitro fertilisation (IVF) cycle in a populatio...

ea0044p207 | Reproduction | SFEBES2016

Bacterial isolates associated with semen and mouth of infertile men

Samuel Titilola , Olagunju Omobonike , Nwhator Segun

Background: This study was aimed at examining the association between the fertility parameters and periodontal status of men, with reference to the bacterial culture analysis of the semen and mouth swab, seminal fluid parameters and blood serum parameters.Method: 43 men consisting 30 infertile men as test subject, and 13 fertile men as control aged 30–50 years were selected for this research. Semen samples were analysed to determine microbial presen...

ea0044p208 | Reproduction | SFEBES2016

Anti-Müllerian Hormone (AMH) and Antral Follicle Count (AFC) are predictive markers in the assessment of patients with menstrual disturbance

Abbara Ali , Clarke Sophie , Roberts Rachel , Vimalesvaran Sunitha , Comninos Alexander , Christopoulos Georgios , Islam Rumana , Franks Steven , Trew Geoffrey , Dhillo Waljit

Background: Anti-Müllerian Hormone (AMH) and Antral Follicle Count (AFC) are both principally used as markers of ovarian reserve and available in all UK hospitals. The utility of these markers in the binary diagnosis of Polycystic Ovarian Syndrome (PCOS) by published criteria, such as Rotterdam, has been previously reported. We evaluated their utility in the evaluation of oligo/amenorrhoea in healthy young non-obese women.Methods: Women with both ov...

ea0044p209 | Reproduction | SFEBES2016

Profiling the decidualisation response of women with endometriosis reveals diverse patterns of steroid responsiveness

Simitsidellis Ioannis , Gibson Douglas A. , Kelepouri Olympia , Horne Andrew W , Saunders Philippa T K

Endometriosis is a chronic hormone-dependent disorder, characterised by growth of ‘ectopic’ endometrial tissue outside the uterus. It has been reported that 30–40% of women who are sub/infertile have endometriosis. Infertility in women with severe endometriosis may be the result of either scarring or pelvic distortion, but in women with mild/moderate disease it is believed subfertility may result from disturbances in endometrial tissue function. Transformation o...

ea0044p210 | Reproduction | SFEBES2016

Safety and tolerability of inducing completion of puberty with IM testosterone over 1 year in older men with congenital hypogonadism and absent puberty

Pazderska Agniezska , Artham Satish , Miller Margaret , Morris Margaret , Ball Steve , Quinton Richard

Background: Guidance on pubertal-induction in hypogonadal adult men is sparse. For adolescent boys, in whom delay is usually constitutional, treatment is typically initiated with pulsed low-dose IM testosterone (T); the dose being progressively increased if/when it becomes clear that endogenous gonadotrophin secretion is not being initiated. In teenagers with organic hypogonadism, the aims are to recapitulate the normal tempo of puberty over 2–3 years and optimise linear ...

ea0044p211 | Reproduction | SFEBES2016

Insight into the molecular mechanisms underlying alterations in gonadotropin receptor activity in polycystic ovarian syndrome

Owens Lisa , Lerner Avi , Sposini Silvia , Christopoulos George , Khanjani Shiran , Islam Razia , Lavery Stuart , Tsui Vicky , Hardy Kate , Franks Stephen , Hanyaloglu Aylin

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder, affecting 5–10% of women of reproductive age, and is the major cause of anovulatory infertility. Aberrant secretion and/or action of gonadotropins are implicated but, to date, we have only limited knowledge about the precise mechanisms involved. Recent genome wide association studies have discovered signals at loci close to the genes coding for gonadotropin receptors. The functional significanc...

ea0044p212 | Reproduction | SFEBES2016

Gonadotrophin secretion is a useful adjunct in the diagnosis of patients with hyperprolactinaemia

Clarke S , Abbara A , Nesbitt A , Ali S , Comninos AN , Hatfield E , Martin NM , Sam A , Meeran K , Dhillo W

Background: Hyperprolactinaemia accounts for 1 in 7 patients presenting with amenorrhoea. Recent data suggests that prolactin acts at the hypothalamus to reduce GnRH-pulsatility. Conditions in which GnRH-pulsatility is reduced, such as hypothalamic amenorrhoea, favour FSH over LH secretion from the pituitary gland. We examined gonadotrophin secretion in hyperprolactinaemic patients as a surrogate marker of GnRH-pulsatility.Methods: A retrospective analys...

ea0044p213 | Reproduction | SFEBES2016

The relation of liver enzymes and insulin resistance in women with polycystic ovary syndrome (PCOS)

Macut Djuro , Bozic-Antic Ivana , Tziomalos Konstantinos , Bjekic-Macut Jelica , Ilic Dusan , Vojnovic-Milutinovic Danijela , Stanojlovic Olivera , Panidis Dimitrios

Introduction: There is a link between polycystic ovary syndrome (PCOS) and nonalcoholic fatty liver disease (NAFLD). Here, we analyzed transaminazes and their relation to insulin resistance (IR) in PCOS women.Methods: We analyzed 600 women with PCOS diagnosed using ESHRE/ASRM criteria (age:25.6±5.9 years, BMI: 30.6±6.9 kg/m2), and 125 BMI-matched healthy controls (age: 31.4±5.3 years, BMI: 29.6±6.8 kg/m2). IR was...

ea0044p214 | Reproduction | SFEBES2016

Correlation of maternal serum Insulin growth factor 1 and 2 to predict foetal outcome

Gupta Amrit , Tiwari Swasti , Singh Nisha

The intrauterine foetal environment is crucial for foetal survival and long term health. The insulin-like growth factors (IGF)-I and -II have a predominant role in fetal growth and development. Insulin-like growth factor 1 (IGF-1) is an anabolic hormone with several biological activities, such as proliferation, mitochondrial protection, cell survival, tissue growth and development.Aims & Objectives: To find the association of Maternal insulin Growth ...

ea0044p215 | Reproduction | SFEBES2016

Fertility effects of Curcumin-a kitchen Spice on Male Wistar Rat

Iranloye Bolanle , Uweru Oghochukwu

Surprisingly, up to 50% of cases of infertility among couples worldwide are male-related. But despite this alarming prevalence of male infertility, most studies continue to focus on analysis of infertility from female perspective. Curcumin (CUM), a food additive with international numbering code E100 has been shown to exhibit therapeutic potential against some illnesses (cancer, diabetes, testicular damage) in which free radicals plays a crucial role (Aggarwal and Harikumar 20...

ea0044p216 | Reproduction | SFEBES2016

Androsterone Glucuronide to dehydroepiandrosterone sulphate ratio is higher in obese Caucasian women with Polycystic Ovary Syndrome

Sathyapalan Thozhukat , Ahmed Lina , Keevil Brian , Miller Adrian , Kilpatrick Eric , Atkin Stephen

Objective: Androsterone glucuronide (ADTG) concentrations have been suggested as a more reliable marker of the effects of androgens at the target tissue level and they are significantly elevated in hirsute compared to non-hirsute women with PCOS. This study compared the different precursors of testosterone, including dehydroepiandrosterone sulphate (DHEAS), ADTG and androstenedione in non-obese compared to obese women with PCOS, and in normal subjects and their implications on...

ea0044p217 | Reproduction | SFEBES2016

A prospective cohort study investigating Endocrine Disrupting Agents and Polycystic Ovary Syndrome within an IVF setting

Cunningham Thomas , Allgar Victoria , Atkin Stephen , Kilpatrick Eric , Maguiness Stephen , Sathyapalan Thozahukat

Background: Endocrine Disrupting Agents (EDAs) are external substances that have the potential to interfere with the natural endocrine pathways such as the reproductive axis. Polycystic ovary syndrome (PCOS) is a common endocrine condition resulting in, hyperinsulinaemia, hyperandrogenaemia and subfertility. This study was conducted to see whether there was any association between EDAs and PCOS.Methods: Blood samples were collected from 59 women (29 PCOS...

ea0044p218 | Reproduction | SFEBES2016

Validation and implementation of a diagnostic NGS panel in Scotland for disorders of sex development

Diver LA , Cerqueira V , Purvis A , Nixon R , Tobias ES , McGowan R , Ahmed SF , Williams N

Disorders of sex development (DSD) are a collection of rare congenital conditions with diverse features and pathophysiology. Patients usually present at birth with atypical genitalia or with delayed puberty in adolescence. Biochemical and cytogenetic investigations may provide guidance on the underlying cause, however molecular genetic analysis is usually required to provide a definitive diagnosis and allows for personalised management of the patient. The current diagnostic se...

ea0044p219 | Reproduction | SFEBES2016

Regulation of the renin-angiotensin system by salt in BeWo and JEG-3 cells

Klossner Raheel , Williams Paula , Eisele Nicole , Kurlak Lesia , Mohaupt Markus , Mistry Hiten

Introduction: The renin-angiotensin system (RAS) become upregulated very early on in pregnancy and is crucial in maintaining blood pressure. In addition to the peripheral RAS there is a uteroplacental RAS, which is also important in regulating placental function and development. Recent work has shown extra-renal sodium storage in the skin; it is suggested that the placenta may also function as a salt sensing organ and is important in regulating maternal blood pressure.<p c...

ea0044p220 | Reproduction | SFEBES2016

Management of Turner’s syndrome women with liver involvement: FIB-4 score is a promising marker of fibrosis

Calanchini Matilde , Moolla Ahmad , Tomlinson Jeremy W , Cobbold Jeremy , Fabbri Andrea , Grossman Ashley , Turner Helen

Introduction: Liver involvement is frequent in Turner’s syndrome (TS). We have shown that 35% TS women have elevated liver function tests (↑LFTs). Most common hepatic changes include steatosis and steatohepatitis; however, progression to advanced fibrosis and cirrhosis is reported. This study assessed a simple noninvasive test for liver fibrosis, FIB-4, which combines standard biochemical values (platelets, ALT, AST) and age in order to evaluate its diagnostic perfo...

ea0044p221 | Reproduction | SFEBES2016

Turner’s syndrome and liver involvement: prevalence and characterisation of a large population with Turner’s syndrome

Calanchini Matilde

Aims: Elevated liver function tests (↑LFTs) are frequent in Turner’s Syndrome (TS). The cause and clinical significance are unclear. The aim of this study was to analyse the association between ↑LFTs and a comprehensive panel of TS-related conditions, focusing on metabolic and cardiovascular diseases in order to further elucidate the pathophysiological mechanisms underlying this condition.Methods: We reviewed our adult TS cohort. LFTs we...

ea0044p222 | Reproduction | SFEBES2016

The Role of the Epidermal Growth Factor (EGF) Receptors (ErbBs) in Mouse Preantral Follicle Development

Thomson Kacie , Atess Victoria , Laird Mhairi , Franks Stephen , Hardy Kate

The factors responsible for regulating primordial follicle activation and early preantral follicle growth remain poorly understood, despite their importance in determining a female’s reproductive lifespan. The essential role of the epidermal growth factor (EGF) family of receptors (ErbBs) during ovulation is well established; however there is emerging evidence that ErbBs may also be important regulators of early follicle development. This study aimed to investigate the ro...

ea0044p223 | Reproduction | SFEBES2016

Outcome of ovarian stimulation for oocyte cryopreservation in women with Turner Syndrome

Talaulikar Vikram , Pimblett Antoinette , Davies Melanie , Conway Gerard

Approximately 20% of women with Turner syndrome (TS) proceed normally through puberty with spontaneous menstruation. An increasing number of women with TS are taking advantage of oocyte cryopreservation which is becoming widely available. It may be expected however, that controlled ovarian stimulation would result in fewer than expected number of oocytes compared to women without TS. We report our initial results from women with TS undergoing this procedure.<p class="abste...

ea0044p224 | Reproduction | SFEBES2016

Case series of 10 46,XX DSD males

Vogazianou Artemis , Deore Mahesh , Conway Gerard

Introduction: 46,XX disorder of sexual development (DSD) is a rare cause of sex reversal. Only a few hundred cases have been described. The clinical spectrum can be divided into 3 groups of males according to genital appearance: normal male, atypical variants such as hypospadias or cryptorchidism, and those with both male and female genitalia.Subcategories of 46,XX DSD include ovotesticular DSD, which is characterised by the presence of both testicular a...

ea0044p225 | Reproduction | SFEBES2016

Spontaneous pregnancy in Turner’s Syndrome: An optimistic analysis

Calanchini Matilde

Aims: Spontaneous pregnancy (SP) in Turner’s syndrome (TS) has been reported, with a prevalence of 2 to 7%. The aim of this study was to evaluate the prevalence and the outcome from spontaneous pregnancies (SPs) in a cohort of women with TS from a single centre, in order to give realistic counselling regarding options for fertility.Methods: We considered the following data: karyotype, age at diagnosis of TS, age at the time of the study, cardiac, an...

ea0044p226 | Reproduction | SFEBES2016

A multidisciplinary specialist team for pregnancy in Turner’s syndrome improves survival and maternal and fetal outcomes

Kumarasinghe Gayathri , Calanchini Matilde , Mackillop Lucy , Weingart Emma , Orchard Elizabeth , Turner Helen

Aims: Turner’s syndrome (TS) is associated with bicuspid aortic valve (BAV), ascending aortic dilatation (AD), aortic coarctation, and hypertension. Pregnancy in TS is associated with increased risk of aortic dissection (2%), gestational hypertension, pre-eclampsia and a 2% risk of maternal mortality.This retrospective study aimed to assess the effectiveness of a multidisciplinary team (MDT) comprising endocrinologist, cardiologist and maternal medi...

ea0044p227 | Reproduction | SFEBES2016

Re-evaluation of safety in pregnancy following oocyte donation in Turner’s Syndrome; is it time to modify the guidelines?

Kumarasinghe Gayathri , Calanchini Matilde , Mackillop Lucy , Weingart Emma , Orchard Elizabeth , Turner Helen

Aims: Oocyte donation (OD) is increasingly utilised in women with Turner’s syndrome (TS). However, guidelines state TS a ‘relative contraindication’ for pregnancy, due to increased risk of aortic dissection (AD 2%) and maternal mortality (2%). Recent data on OD-related morbidity and mortality in TS has raised further concern. We aimed to analyse cardiovascular risk profiles of TS women undergoing OD and those with spontaneous pregnancy (SP), and determine outcom...

ea0044p228 | Reproduction | SFEBES2016

Causes of primary amenorrhea in women evaluated in an academic center of adult endocrinology

Gheorghiu Monica Livia , Cucu Constantin , Trifanescu Raluca Alexandra , Badiu Corin

Introduction: Primary amenorrhea is defined as the absence of menses at age 15 years in the presence of normal growth and secondary sexual characteristics, or at 13 years, if there is no breast development. We retrospectively assessed the causes of primary amenorrhea in a series from an academic center of adult endocrinology.Patients and methods: We retrieved data from the files of 111 consecutive patients with primary amenorrhea evaluated in our center ...

ea0044p229 | Reproduction | SFEBES2016

Effect of predator-induced psychosocial stress on implantation and pregnancy outcome in rats

Iranloye Bolanle , Medubi Oluwatoyin , Adegoke Olufeyisipe

Maternal stress is commonly cited as a potential cause for idiopathic pregnancy loss (Knackstedt et al., 2005). However, the mechanisms through which stress affects implantation and pregnancy are yet to be totally deciphered. This study was designed to determine the effect of predator-induced psychosocial stress on implantation and pregnancy in rat. Cycling rats (n=48) at proestrus phase were paired overnight with sexually experienced male in ratio 2:1. Following conf...

ea0044p230 | Reproduction | SFEBES2016

Serum total cholesterol, thyroid hormone concentrations and haematological variables in cyclic and acyclic Nili-Ravi buffaloes

Ghani Muhammad Usman , Ahmad Ijaz , Ahmad Nazir , Mehfooz Ashar , Ijaz Nabeel

Buffalo is a major dairy animal in many countries of the world, including Pakistan. However, the productive and reproductive performance of this species is affected by many physiological problems such as anestrous. Therefore, this study was conducted to compare serum total cholesterol, thyroid hormone concentrations and haematological variables in cyclic and acyclic Nili-Ravi buffaloes. For this purpose, 60 adult Nili-Ravi buffaloes were divided into two equal groups i.e. cycl...

ea0044p231 | Reproduction | SFEBES2016

Hormonal profiling to detect male mini-puberty: a rapid and accurate diagnostic approach in suspected cases of congenital hypogonadotrophic hypogonadism

Mamoojee Yaasir , Cheetham Tim , Murdoch Alison , Quinton Richard

Activation of the hypothalamo-pituitary-gonadal axis, from the third trimester of pregnancy to the first post-natal months in males, results in serum concentrations of gonadotrophins and testosterone approaching adult levels. This phase, known as male mini-puberty, represents a key window of opportunity to identify congenital GnRH deficiency in early childhood.We present a case to illustrate the diagnostic efficiency of screening for mini-puberty in a ma...

ea0044p232 | Reproduction | SFEBES2016

Levels of reactive oxygen species (ROS) in the seminal plasma predicts the effectiveness of L-carnitine to improve sperm function in men with infertility

Vessey Wayne , McDonald Cassandra , Virmani Ashraf , Almeida P. , Jayasena Channa , Ramsay Jonathan

Background: Oxidative stress is implicated in the pathogenesis of primary male infertility. Some studies suggest that administration of the amino acid derivative anti-oxidant, L-carnitine, may improve sperm quality. No previous study has investigated whether the anti-oxidant effects of L-carnitine relate to changes in sperm quality in men with infertility.Aim: Investigate whether L-carnetine significantly improves sperm function, and whether baseline lev...