SFEBES2016 Oral Communications Diabetes Mellitus and Metabolism (6 abstracts)
Depot specific transcriptional signatures of adipose tissue in sheep and humans during early life
Hernan Fainberg 1 , Shalini Ojha 1 , Ahmad Alhaddad 1 , Reham Alagal 1 , Mark Birtwistle 1 , Graeme Davies 1 , Marcos Castellanos 2 , Sean May 2 , Giussepe Pelella 3 , Attilio Lotto 3 , Harold Sacks 4 , Helen Budge 1 & Michael Symonds 1
1Division of Child Health, Obstetrics & Gynaecology, School of Medicine, Queen’s Medical Centre, University Hospital, The University of Nottingham, Nottingham, UK; 2Nottingham Arabidopsis Stock Centre, School of Biosciences, The University of Nottingham, Sutton Bonington, UK; 3University Hospitals NHS Trust, Leicester, UK; 44VA Endocrinology and Diabetes Division, VA Greater Los Angeles Healthcare System, and Department of Medicine, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, USA.
The identification and characterisation of unique gene profiles expressed in specific adipose tissue depots around the body could provide novel insights on fat development.
We report a comprehensive analysis of transcriptome from the five major (epicardial, pericardial, perirenal, sternal and omental) adipose depots from one week old sheep. This study also provides an integrated view of the preservation and differences between mitochondrial gene co-expression networks found in each adipose depot in relation to paediatric human epicardial adipose tissue (EAT). Based on microarray analysis, we identified novel adipose depot-specific gene expression patterns for a significant portion of transcripts (~10%). By weighted gene co-expression network analysis, we observed that each depot can be delineated concisely by a small number of functional modules of co-expressed genes. This result indicates a consistent transcriptional change in each depot in pathways indicative of different cellular origin, metabolism and thermogenic functionality. Cross-species comparisons of mitochondrial genes with human paediatric EAT revealed that the modules encapsulating mitochondrial functional and structural composition were preserved in 3 out of 5 ovine adipose depots. However, the majority of DNA and RNA transcriptional regulation, as well as the reactive oxygen cell defence module, differed between humans and sheep. Furthermore, we identified that the key module confining mitochondrial thermogenic activity showed negative correlation with the child’s weight and height. Together, the results provide unique information of regulatory mechanisms underlying the adaptable morphology of adipose tissue over time and location.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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