Endocrine Abstracts

Introduction: mTOR is not only a central regulator of lipid metabolism, controlling the processes of adipogenesis and lipolysis, but also a regulator of immunometabolism of immune cells infiltrating the adipose tissue. In its turn, the level of progression of diabetes is largely limited by Treg subpopulation, the complexity and heterogeneity of which is confirmed by the detection of numerous tissue-specific Tregs, including the so-called VAT Tregs (visceral adipose tissue CD4+Foxp3+ regulatory T cells). Therefore, the purpose of the work was to find out the level of expression of mRNA genes of mTOR, Foxp3, IL1β and IL17A in parapancreatic adipose tissue of rats with experimental streptozotocin-induced diabetes after introduction of metformin.

Methods: We use RT-PCR method for investigating of mRNA expression levels of genes mTOR, Foxp3, IL1β and IL17A. To determine the level of target genes was performed RT-PCR in real-time by thermocycler CFX96™ Real-Time PCR Detection Systems. The relative level of gene expression were studied with rat reference genes GAPDH by the method _ΔΔCt. Statistical analysis were conducted using available software «Bio-Rad CFX Manager 3.1» (Bio-Rad, USA).

Results: the development of diabetes causes the transcriptional activation of the gene of the protein kinase mTOR, does not affect the expression of mRNA of Foxp3, increases the level of expression of mRNA of proinflammatory cytokines IL1β and IL17A. At the same the introduction of metformin in diabetic rats inhibits the expression of mRNA of mTOR and increases the level of transcriptional activity of the gene Foxp3 in parapancreatic adipose tissue.