Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2017) 49 EP795 | DOI: 10.1530/endoabs.49.EP795

ECE2017 Eposter Presentations: Interdisciplinary Endocrinology Female Reproduction (3 abstracts)

Comparison of free testosterone results by a radioimmunoassay and a chemiluminescent assay

Conceicao Godinho & Isaura Rodrigues

Centro Hospitalar Lisboa Central, Lisboa, Portugal.

Introduction: Radioactive assays are widely used in clinical laboratories for measuring free testosterone. The appearance of a non-isotopic, chemiluminescent automatic assay, may be an interesting alternative if the performance of the assay remains the same or higher.

Objective: To compare the free testosterone results by radioimmunoassay (RIA) and chemiluminescent assay (CLIA) and to analyze the clinical impact taking into account the % concordance.

Material and Methods: We studied 76 patients, aged 13–73 years old, and observed at CHLC medical appointments, in Lisbon. The serum samples were analyzed by “Free TESTO-RIA-CT”, DIASource (0.2–22.1 pg/ml) and “Maglumi Free Testosterone (CLIA)” Snibe (1.52–40.62 pg/ml). An excel tool was used for statistical treatment and the clinical concordance of the results were analyzed according to the reference values of both.

Results: A strong correlation was observed r=0.95 and linear regression was calculated: y=1.51x+0.072. The % concordance found was:

n Age Agreement Disagreement% concordance

Conclusion: There is a good agreement between the two methods. The absence of age-stratified reference values by CLIA’ method may explain the discordant results. Despite the small sample size, Maglumi free testosterone may be considered a good alternative to the RIA assay, although it is fundamental to have reference values according to age and gender since the concentration of free testosterone varies throughout life from childhood to adulthood.

Volume 49

19th European Congress of Endocrinology

Lisbon, Portugal
20 May 2017 - 23 May 2017

European Society of Endocrinology 

Browse other volumes

Article tools

My recent searches

No recent searches.