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Endocrine Abstracts (2017) 49 GP24 | DOI: 10.1530/endoabs.49.GP24

1Group of Endocrine Disorders, Department of Endocrinology Hospital Clinic Barcelona, IDIBAPS, CIBERDEM, Barcelona, Spain; 2Department of Endocrinology, Hospital de Girona ’Dr Josep Trueta’; IdIBGi Girona, CIBERobn, Girona, Spain; 3Biochemistry and Molecular Genetics, Hospital Clinic Barcelona, Barcelona, Spain.


Exosomes are nano-sized vesicles secreted by the cells in the exatracellular fluids representing a novel way of intracellular communication. Exosomes secreted from blood cells like monocytes and platetes have been reported to be checkpoints involved in haemostatic and thrombotic response. Glucocorticoids are inducing a profound alteration of blood cells homeostasis with atherothrombotic, bleeding and immune response alterations. Hereby we quantified for the first time circulating exosomes in overt Cushing syndrome (CS) patients.

Methods: Cross sectional study in CS patients and controls. Diagnosis of CS was performed following ECE and Endo guidelines. Blood samples from active, drug naive CS patients (n=20) and age and sex matched controls (n=20) were obtained after overnight fast. Peripheral blood cells, biochemical and hormonal parameters were analyzed using standard methods. Circulatory exosomes were quantified in plasma by a sandwich enzyme-linked-immuno-sorbent assay (ExoTEST) employing a monoclonal antihuman CD9 antibody. Further exosome phenotyping and analysis is ongoing.

Results: CS patients present as compared to controls a significant increased number of CD9 positive exosomes (2.35×1010 vs 7.74×109/100 μl; P=0.005**). Absolute monocytes (MN) number and 24 h urinary free cortisol (UFC) level correlates with the number of exosomes, respectively P=0.022* with r:0.391 for MN and P=0.005** with an r:0.467 for UFC.

Conclusion: Circulatory exosomes are increased in the hypercortisolemic state of Cushing syndrome.

Volume 49

19th European Congress of Endocrinology

Lisbon, Portugal
20 May 2017 - 23 May 2017

European Society of Endocrinology 

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