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Endocrine Abstracts (2017) 49 OC2.5 | DOI: 10.1530/endoabs.49.OC2.5

Diabetes Prediction and Complications

Urinary peptidomics for the detection of diabetic kidney disease

Leticia A Brondani, Ariana A Soares, Angelica D’Allagnol, Joíza L Camargo, Karina Monteiro & Sandra P Silveiro

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Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.


Background: Diabetic kidney disease (DKD) is the main cause of end-stage renal disease It is defined by glomerular filtration rate (GFR) impairment and/or presence of increased urinary albumin excretion (UAE). However, these parameters are nonspecific and somewhat delayed manifestations of renal damage. Thus, earlier DKD new biomarkers are strongly warranted.

Objective: To investigate the urinary peptidomics profile of type 2 diabetes mellitus (DM) patients with different stages of DKD.

Methods: Casual urine samples were collected from 66 type 2 DM patients matched by age, gender and time of diabetes duration. Urine natural occurring peptides were purified by ultrafiltration under denaturing conditions and analyzed by LC–MS/MS. UAE was assessed by immunoturbidimetry and GFR was estimated by CKD-EPI equation. Kruskall–wallis, Mann–Whitney and χ2 tests were performed when appropriate; Perseus software was used to perform hierarchical clustering of significantly up- and down-regulated proteins.

Results: Type 2 DM patients (mean age=−61.5±9.7 years; males=47.1%) were stratified by the levels of albuminuria (normal (n=27), moderately increased (MI, n=18) and severely increased (SI, n=21)). A total of 116 urinary proteins were detected by LC/-MS/MS. A distinct proteomic profile was identified in patients with SI albuminuria, represented by 11 proteins. When GFR values were analyzed, we observed that 13 urinary proteins differed significantly in the 9 patients with GFR <60 ml/min per 1.73 m2 when compared to 57 patients with GFR ≥60 ml/min per 1.73 m2. Among the most remarkably different urinary protein profile, alpha-1 type I collagen was around 10% less expressed in SI patients, while alpha-1 antitrypsin, plasma protease C1 inhibitor and apolipoprotein-1 were ~twofold increased in these patients.

Conclusions: LC–MS/MS analysis revealed that at least 11 urinary proteins were differentially expressed in type 2 DM patients according to DKD severity. This study confirms the previously described findings in other populations and also detected novel proteins commonly altered in patients with GFR impairment and increased albuminuria.

Volume 49

19th European Congress of Endocrinology

Lisbon, Portugal
20 May 2017 - 23 May 2017

European Society of Endocrinology 

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