Background and Objective: Several animal studies showed the expression of peroxisome proliferator activated receptor-gamma (PPAR gamma) in adipocytes among rats fed with high-fat diet was higher than those fed with normal diet. However, there are no human studies to support this. The objective of the present study was to investigate the association between the PPAR gamma expression in adipose tissue and intake of hydrogenated and non-hydrogenated vegetable oils and butter.
Materials and methods: Visceral and subcutaneous adipose tissues were obtained from 97 non-diabetic subjects (41 non-obese, 18 obese, and 38 morbid obese), who underwent open abdominal surgery with minimal impact on dietary intake. Intake of hydrogenated and non-hydrogenated vegetable oils and butter was collected using a valid and reliable food frequency questionnaire. The gene expressions of PPAR-gamma in visceral and subcutaneous adipose tissue were assessed by Real-Time PCR.
Findings: After adjustment for total energy intake and age, visceral adipose tissue PPAR-gamma gene expression was correlated with total fat and oil intake (β=−0.531, P=0.014) in total population. Among non-obese subjects, visceral adipose tissue PPAR-gamma gene expression was correlated with non-hydrogenated oil (β=0.621, P=0.017). PPAR-gamma gene expression in subcutaneous adipose tissue was correlated with non-hydrogenated oil (β=0.481, P=0.026) among obese subjects. Moreover, among morbid obese subjects we found significant correlations between visceral adipose tissue PPAR-gamma expression and total fat and oil intake (β=0.417, P=0.020).
Conclusions: Expression of adipose tissue PPAR-gamma mRNA negatively correlates with total fat and oil intake mostly in visceral fat. In addition, non-hydrogenated oil positively correlates with PPAR-gamma expression.
20 - 23 May 2017
European Society of Endocrinology