Objective: To compare 3 methods of generating Ki-67% in gastroenteropancreatic neuroendocrine tumours (GEP-NETs).
Methods: Twenty-four slides were used to generate 49 images for analysis. Each image was analysed and 3 different methods were used to calculate Ki-67%.
Results: The comparison of Ki-67% from counting and estimating using the diameter (shortcut method) was strongly positively correlated, whereas the Ki-67% from counting and using immunoratio were only weakly positively correlated.
Conclusion: Guidance from The Royal College of Pathologists 2017 states that clinicians must now count 2000 cells from hotspots within the tissue sample to calculate a Ki-67%. The shortcut method gives reasonably accurate results, the online immunoratio is less reliable.
04 Dec 2017
UK and Ireland Neuroendocrine Tumour Society