Objective: Vitamin D is a multifunctional hormone with possible antitumor effects. Its exact role in breast carcinogenesis remains largely unknown. We evaluated serum vitamin D levels in female patients with recently identified benign or malignant lesions of the breast and its associations with tumor histology.
Subjects-methods: One hundred sixty women with suspicious breast masses that required surgical excision were included in this study. Body composition data were measured using bioelectrical impedance. Pre-operatively, patients provided early morning blood sample for the quantification of Vitamin D status and other biochemical parameters. Determination of vitamin D status of the patients was implemented by quantification of 25-hydroxyvitamin D3 and D2 metabolites (25OH-D3/D2) in serum samples of the patients by our previously developed and validated HPLC-DAD method (1). Surgically excised lesions were sent for histological examination and histopathological characterictics were recorded.
Results: After histological examination of the tumor specimens, 64 specimens were found with benign histology and 96 specimens had malignant characteristics. Comparisons between study groups are shown in Table 1. Cancer patients were older and had greater waist circumference. Vitamin D3 levels were similar in patients with benign lesions vs. those with malignancy. Regarding associations with histological characteristics, Vitamin D3 was associated with nodular metastasis (ρ=0.26, P=0.03) and Ki67 levels (ρ=0.27, P=0.02) but did not correlate with tumor size (ρ=0.06, P=0.62). No significant associations were found with overall TNM staging, presence of estrogen or progesterone receptors and Her-2 protein (P>0.05 for all).
|Variable||Benign Group||Cancer Group||P|
|Body Mass Index (kg/m2)||27.78±5.86||28.98±5.17||0.18|
|Waist Circumference (cm)||89.62±14.63||93.80±13.10||0.03|
|Hip Circumference (cm)||104.45±12.46||107.00±11.13||0.10|
|Total body fat (%)||36.93±8.34||39.22±9.57||0.07|
|Total Cholesterol (mg/dl)||255.19±259.71||214.98±41.61||0.14|
|Vitamin D3 (ng/ml)||19.92±9.87||18.94±11.17||0.58|
Conclusions: Vitamin D was associated with tumor aggressiveness. Its role as a non-invasive biomarker of breast cancer prognosis warrants further investigation in longitudinal studies and/or clinical trials.
Reference: 1. D. Palaiogiannis et al. J Chromatogr B Analyt Technol Biomed Life Sci, 1043 (2017) 219-227. PMID:27756628.
19 May 2018 - 22 May 2018