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Endocrine Abstracts (2018) 56 P640 | DOI: 10.1530/endoabs.56.P640

ECE2018 Poster Presentations: Interdisciplinary Endocrinology Diabetes (to include epidemiology, pathophysiology) (5 abstracts)

The 4-hydroxynonenal mediated oxidative damage of blood proteins and lipids involves secondary lipid peroxidation reactions

Mahmoud A. Alfaqih 1 , Ayman G. Mustafa 1, & Othman Al-shboul 1



Lipid peroxidation is linked with several metabolic diseases. Lipid peroxidation causes cellular damage through reactive aldehyde species such as 4-hydroxyonenal (4-HNE). The exact mechanism(s) by which 4-HNE causes damage in the intravascular compartment is not exactly known. Using an in vitro system, we investigated the damage induced by 4-HNE on the blood by measuring protein carbonyl groups and thiobarbituric acid reactive substances (TBARS) following 4-HNE treatment. We showed that treatment with 4-HNE increased the carbonylation of proteins and the formation of TBARS in the blood plasma. We also tested whether phenelzine, a scavenger of aldehyde species, or U-83836E, a scavenger of lipid peroxy radicals, attenuates the damage caused by 4-HNE. We showed that phenelzine or U-83836E can both mitigate the effects of 4-HNE on the proteins and the lipids of the blood plasma. We explained the above results through a model that involves secondary lipid peroxidation reactions initiated by 4-HNE.

Volume 56

20th European Congress of Endocrinology

Barcelona, Spain
19 May 2018 - 22 May 2018

European Society of Endocrinology 

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