The kisspeptin/Kiss1R and tachykinin 3 (TAC3)/tachykinin receptor 3 (TACR3) system in the hypothalamus are essential for reproduction. Recent study suggested that the two peptide systems are both expressed in the ovary, particularly in the granulosa cells. The expression profile of the two systems in human granulosa cells has not been fully investigated yet. We collected the granulosa cells from normal infertile control patients and patients diagnosed with PCOS in Peking University Third Hospital. The expression of Kiss1, Kiss1R Tac3, and Tacr3 increased at 2000 when compared with 1000 in granulosa cells from normal control patients. While the expression of Kiss1 and Tacr3 increased, Kiss1R and Tac3 decreased at 2000 when compared with 1000 in patients with PCOS. HCG had no significant effect on the expression of Kiss1, Kiss1R Tac3, and Tacr3, but significant promoted the expression of circadian time gene PER1. Kisspeptin-10 and Tacr3 antagonist, senktide decreased the expression of circadian gene Bmal1 and per2, but it was not statistically significant. The expression of Kiss1 was related to the expression of Bmal1 at different time within a day. Our results suggest that ovarian kisspeptin/Kiss1R system and Tac3/Tacr3 system have a different expression profile in patients with and without PCOS. The discrepancy may be related to the different circadian clock gene expression manner in the granulosa cells.
19 - 22 May 2018
European Society of Endocrinology