Elongation factor, RNA polymerase II, 2 (ELL2) is an RNA Pol II elongation factor with functional properties similar to ELL that can interact with the prostate tumor suppressor ELL-associated factor 2 (EAF2). In murine models, deletion of Ell2 induced prostate intraepithelial neoplasia similar to that induced by deletion of Eaf2. Since ELL2 and EAF2 can functionally interact and appear to have similar function in regulating prostate homeostasis, we investigated the impact of combined deletion of ELL2 and EAF2 in prostate cancer cell lines. Combined deletion of ELL2 and EAF2 did not result in an additive increase in proliferation, invasion or migration in prostate cancer cell lines. Combined knockdown of ELL2 and EAF2 induced an additive increase in p-AKT activation. These results suggest that ELL2 and EAF2 can compensate for each other in the regulation of cellular proliferation, but that they work together to regulate the AKT pathway.
19 Nov 2018 - 21 Nov 2018