ECE2020 Audio ePoster Presentations Bone and Calcium (121 abstracts)
Bone quality is associated with perinatal nutritional manipulation in adult Wistar rats offspring
Areti Augoulea 1 , Nikolaos Syggelos 1 , Eleni Armeni 1 , George Kaparos 2 , Homeira Vafaei 3 , Ismene Dontas 4 , Katerina Marinou 5 , George Vaggos 6 , Panagiota Raptou 4 , Irene Lambrinoudaki 1 , Makarios Eleftheriades 1,7 & Kypros H Nicolaides 7
1National and Kapodistrian University of Athens, Aretaieio Hospital, 2nd Department of Obstetrics and Gynecology, Athens, Greece; 2National and Kapodistrian University of Athens, Aretaieio Hospital, Hormonal and Biochemical Laboratory, Athens, Greece; 3Faghihi Hospital, Shiraz University of Medical Sciences, Department of Obstetrics and Gynecology, Shiraz, Iran; 4National and Kapodistrian University of Athens, Laboratory for Research of the Musculoskeletal System, Th. Garofalidis, KAT Hospital, Athens, Greece; 5Greek Ministry of Rural Development and Food, Department of Animal Welfare for farm and laboratory animals, Athens, Greece; 6General Hospital of Arta, Department of Obstetrics and Gynecology, Arta, Greece; 7King’s College Hospital, Harris Birthright Research Centre for Fetal Medicine, London, United Kingdom
Introduction: Prenatal and perinatal food manipulation might be related with bone quality in offspring of Wistar rats. This study aimed to assess the impact of different dietary patterns provided before delivery to the mothers on the skeletal characteristics of newborn rats and the levels of insulin.
Materials and methods: We randomized 67 timed-pregnant rats into control diet (CD), high-fat (FD) and food-restricted (FR) on the 12th day of gestation. The pregnant Wistar rats delivered 618 pups on the 21st day. We classified pups born to FR-mothers, based on their birthweight, into fetal-growth restricted (FGR) and non-FGR. Following delivery, we enforced postnatal food manipulation for all pups; the pattern of cross-fostering consisted of the offspring of CD-mothers being now lactated by FD, FR or CD fed dams. A similar process was applied for offspring of FR and FD-mothers. All pups were weaned to the diet of their fostered mother, starting from the 26th postnatal day until their 1st year of age, when they were sacrificed. We measured biochemical parameters and skeletal properties, using peripheral quantitative computed tomography.
Results: FGR-pups that received CD after birth had better skeletal properties compared to FGR-offspring that were starved postnatally and lower values of total/subcortical area compared to controls. Offspring fat-restricted postnatally had better skeletal properties if born to mothers who received high-fat diet compared to mothers who were food restricted during gestation. Higher levels of insulin were evident in pups born to mothers who received high-fat diet during gestation, provided pups received high-fat diet rather fat-restriction after birth.
Conclusion: The results of this study confirm the significance of intrauterine diet for the establishment of appropriate skeletal properties of the neonate. Islet dysfunction and predisposition towards raised insulin production during lifetime is linked with high-fat intake both before and particularly after birth.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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