Endocrine Abstracts

Perinatal action of estrogens or aromatizable steroids at the central nervous system level is responsible for brain development. 4-tert-octylphenol (OP) is knows as an endocrine disrupting chemical (EDC) that is widespread estrogenic chemical used in the costumer produces. Thus, we hypothesis that through early contact with the central nervous system, OP could alter the processes affecting behavior. In this study, primary cortical neurons were exposure to OP (10^–6 or 10^–8 mol/l) from day in vitro 1 to 4 with/without estrogen antiestrogen ICI 182 780. In in vivo, we studied the negative impacts of OP on the neuro behavioral development in the offspring generation from OP-supplementation dams (10 or 50 mg/kg on GD9.5 to PND28). Primary cortical neurons were exposure to OP showed increased in the numbers of primary and secondary dendrites. There was no difference in the number ofprimary and secondary axons in OP-treated groups compared to vehicle group. OP-treated groups displayed decreased in the average length both of primary axons and dendrites. Co-treated OP with ICI reversed effect of OP. At 6–8 week-aged, off spring mice from OP-supplementation dams showed limited in acquisition of spatial reference memory and a decreased capability of locating the target in the probe trial of Morris water maze task. In addition, OP-treated groups showed cognition dysfunction in novel test. Moreover, OP-treated groups exhibited impairments in sociability and social novelty preference in three-chamber social test and social interaction test. In open field test, OP-treated groups increased anxiety-like behavior and change the locomotor activity in mice. Our results first pronounced that exposure to perinatal exposure to OP influences the neuronal development, lead to abnormal behavior in the adult off spring mice.