Searchable abstracts of presentations at key conferences in endocrinology
Endocrine Abstracts (2020) 70 EP401 | DOI: 10.1530/endoabs.70.EP401

1Laboratorio Analisi Cliniche ‘Gentile S.A.S.’, Field of research ‘Gentile S.A.S.’, Gragnano, Italy; 2Günè Centro Medico, Acerra, Italy; 3Clinica HERA, Giugliano in Campania, Italy; 4Hospital San Francisco de Asis, Oliveto Citra, Italy


Introduction: Recent studies report the presence of KLK3 in women serum, KLK3 is excreted by Skene glands, it is involved in the production of cervical-vaginal fluid (CVF). Activation of KLK3 is pH depending, it has been hypothesized to have antimicrobial, immuno-regulator activity and vaginal and cervical epithelial desquamation. We intend to verify if KLK3 is present in female serum, in which menstrual cycle phases KLK3 increases and its main role.

Materials and methods: They were enrolled voluntary young women aged between 21 and 44 years with specific requirements: no professionally exposed, no alcohol, no smoking, no drugs, no use of oral contraceptives for at least 2 years and regular menstrual cycles.To the participants was asked the age of the menarche, if they are nulliparous/multiparous and if they have had spontaneous/volunteer abortions. BMI (body mass index) and the Ferriman-Gallwey score were calculated. Blood samples were taken in three periods of the cycle (days: 5/6; 12/13; 19/20) for the plasma assay of total KLK3 and in the third sample the Progesterone test was performed. The method used is ‘Access Hybritech PSA’ test. The total KLK3 values are expressed in ng/ml.

Results: KLK3 showed a non-homogeneous behaviour, in fact out of 82 participants, 27, equal to 32.9%, can’t be measured in any of the three samples, 55, equal to 67.1% gave values measurable. The comparison of total KLK3 values with the anamnestic data and semeiotic evaluations didn’t give significant correlations. We subdivided the Progesterone values into two bands: band A with Progesterone values > 14.5 ng/ml and Band B with Progesterone values < 14.5 ng/ml. In band B, from the values of the first sample there is a decrease in the second sample and then a constant decrease in the third one. Instead in band A, there is a continuous increase. The curve of the total KLK3 values for multiparas is comparable to that of Progesterone (band A). This could demonstrate an interaction between the total KLK3 and Progesterone.

Conclusion: The transition between the ovulatory and the lutein phase is the key to understanding the quality of ovulation. Measuring KLK3 in the days before ovulation, it would give us indications on how ovulation will be. KLK3 values variation, as in the Profile A, indicates an excellent ovulation that could be different from the one with decreasing values (Profile B). In fact, the curve of KLK3 in Profile A is comparable with that of the multiparas.

Volume 70

22nd European Congress of Endocrinology

Online
05 Sep 2020 - 09 Sep 2020

European Society of Endocrinology 

Browse other volumes

Article tools

My recent searches

No recent searches.

My recently viewed abstracts