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Belgian Endocrine Society 2020

Online, Online
11 Nov 2020 - 11 Nov 2020

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BES 2020

Purification of pancreatic endocrine subsets reveals increased iron metabolism in beta-cells

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Intestinal delivery of proinsulin and IL-10 via Lactococcus lactis combined with low-dose antiCD3 induces antigen-specific FoxP3+ Tregs in autoimmune diabetic mice
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ea0071005 | Purification of pancreatic endocrine subsets reveals increased iron metabolism in beta-cells | BES2020

Purification of pancreatic endocrine subsets reveals increased iron metabolism in beta-cells

C Berthault , W Staels , R Scharfmann

Objectives: The main endocrine cell types in pancreatic islets are alpha-, beta-, and delta-cells. Although these cell types have distinct roles in the regulation of glucose homeostasis, inadequate purification methods preclude the study of cell-type-specific effects. We aimed to develop a reliable approach that enables simultaneous sorting of live alpha-, beta-, and delta-cells from mouse islets for downstream analyses.Methods: We developed an antibody-...
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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