ea0071005 | Purification of pancreatic endocrine subsets reveals increased iron metabolism in beta-cells | BES2020
, W Staels
, R Scharfmann
Objectives: The main endocrine cell types in pancreatic islets are alpha-, beta-, and delta-cells. Although these cell types have distinct roles in the regulation of glucose homeostasis, inadequate purification methods preclude the study of cell-type-specific effects. We aimed to develop a reliable approach that enables simultaneous sorting of live alpha-, beta-, and delta-cells from mouse islets for downstream analyses.Methods: We developed an antibody-...