SFEBES2021 Oral Communications Endocrine Cancer and Late Effects (6 abstracts)
Transcriptomic analysis of succinate dehydrogenase subunit deleted cells to identify molecular mechanisms underlying the increased metastatic potential of SDHB-deficient tumours
Grace Salsbury , Charlotte Hall , Eugenie Lim , Jordan Read , Scott Akker & Paul Chapple
Queen Mary University of London, London, United Kingdom
Mutations in each of the 4 subunits of succinate dehydrogenase (SDH) – SDHA, B, C and D predispose to development of phaeochromocytomas and paragangliomas. Loss of SDH function leads to accumulation of succinate which acts as an oncometabolite to drive tumourigenesis. However, mutations in SDHB have an increased likelihood of causing metastatic disease, compared to mutations in the other SDH subunits. The reasons behind this increased risk remain elusive due to the rarity of these tumours and heterogeneity in patient phenotype. To address this, we have used CRISPR/Cas9 to generate a series of isogenic SDH knockout cell lines as a tool to investigate the cellular consequences of loss of specific subunits. Characterisation of these cell lines demonstrates that they recapitulate the phenotypes reported for previous SDH knockout models and tumour tissue. Transcriptomic analysis has identified differentially expressed gene signatures that are unique to SDHB knockout cells, potentially identifying mechanisms responsible for the increased risk of metastasis in SDHB- deficient tumours.
Volume 77
Article tools
My recent searches
My recently viewed abstracts
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2024 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more