Searchable abstracts of presentations at key conferences in endocrinology
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European Congress of Endocrinology 2022

Milan, Italy
21 May 2022 - 24 May 2022

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The 24th European Congress of Endocrinology (ECE) and the Italian Society of Endocrinology look forward to welcoming you to Milan. ECE 2022 returns for a four day, six parallel session festival of endocrine science. You can join us In person in Milan, Italy OR Live @Home online!

Poster Presentations

Reproductive and Developmental Endocrinology

ea0081p180 | Reproductive and Developmental Endocrinology | ECE2022

European Registries for Rare Endocrine Conditions (EuRRECa): results from the e-Reporting platform for rare conditions (e-REC)

Rashid Ali Salma , Bryce Jillian , Luisa Priego Zurita Ana , Cools Martine , Danne Thomas , Katugampola Harshini , Dekkers Olaf M. , Hiort Olaf , Linglart Agnes , Netchine Irene , Nordenstrom Anna , Patocs Attila , M Pereira Alberto , Persani Luca , Reisch Nicole , Smyth Arelene , Sumnik Zdenek , Taruscio Domenica , Visser Edward , Appelman-Dijkstra Natasha , Ahmed Faisal

Background: EuRRECa (eurreca.net) is a web-based project that supports professional networks such as European Reference Networks (ERNs) in capturing standardised clinical information. The project includes an e-reporting registry (e-REC), which can be used to perform regular surveillance of specific events. Since 2018, e-REC has been used by the ERN for rare endocrine conditions (Endo-ERN) to understand the number of new clinical encounters at centres within this network.<p...

ea0081p181 | Reproductive and Developmental Endocrinology | ECE2022

Hormonal treatment modification during the long term follow-up of transwomen and transmen individuals: a retrospective observational Italian study

Cacciani Marta , Spaggiari Giorgia , M. Granata Antonio R. , Simoni Manuela , Santi Daniele

Background: Persons with assigned either male (AMAB) or female (AFAB) sex at birth might wish to obtain feminization or masculinization, respectively. To this purpose, hormonal treatment with sex hormones must be tailored to each subject. Several studies and the European Society of Endocrinology guidelines tried to identify the optimal hormonal treatment in both AMAB and AFAB subjects. However, the clinical management in the long term follow-up remains challenging. Many treatm...

ea0081p182 | Reproductive and Developmental Endocrinology | ECE2022

Defining reference ranges for serum anti-Müllerian hormone (AMH) on a large cohort of normozoospermic adult men highlights new potential physiological functions of AMH on FSH secretion and sperm motility

Benderradji Hamza , Anne-Laure Barbotin , Maryse Leroy-Billiard , Julie Prasivoravong , Francois Marcelli , Christine Decanter , Geoffroy Robin , Valerie Mitchell , Jean-Marc Rigot , Antonino Bongiovanni , Florent Sauve , Luc Buee , Claude-Alain Maurage , Maryse Cartigny , Arnauld Villers , Vincent Prevot , Sophie Catteau-Jonard , Nicolas Sergeant , Paolo Giacobini , Pascal Pigny , Clara Leroy

Background: Few studies to date have attempted to measure serum anti-Müllerian hormone (AMH) levels in adult men, and solid references ranges have not yet been defined on a large cohort.Objective: In this study, we aimed to first establish the reference ranges for serum AMH and AMH-to-total testosterone ratio (AMH/tT) in adult males. Secondly, we investigated the relationship between serum AMH and both reproductive hormones and semen parameters....

ea0081p183 | Reproductive and Developmental Endocrinology | ECE2022

Intranasal kisspeptin administration stimulates reproductive hormone secretion in healthy volunteers and patients with hypogonadism

Mills Edouard , Swedrowska Magda , Thurston Layla , Phylactou Maria , Patel Bijal , Yang Lisa , Clarke Sophie , Muzi Beatrice , Alexander Emma , Choudhury Muhammad , Bech Paul , Abbara Ali , Forbes Ben , Comninos Alexander , Dhillo Waljit

Background: Kisspeptin is a critical activator of hypothalamic gonadotrophin releasing hormone (GnRH) neurons and has significant potential to treat common reproductive disorders. To date, kisspeptin has solely been administered to humans via the intravenous or subcutaneous routes, however intranasal administration could offer a novel non-invasive delivery route. We therefore sought to determine the effects of intranasal kisspeptin on reproductive hormone release in humans for...

ea0081p184 | Reproductive and Developmental Endocrinology | ECE2022

Effects of testosterone therapy on bone turnover markers and bone mineral density in obese males with type 2 diabetes and functional hypogonadism

Groti Antonic Kristina

Aims: Both functional hypogonadism (FH) and type 2 diabetes (T2D) negatively affect bone mineral density (BMD). We aimed to evaluate changes on bone turnover markers (BTMs) and BMD in obese males with FH and T2D due to testosterone therapy (TTh).Research Design and Methods: 55 obese males with FH and T2D participated in a two-year (first year double-blind, placebo-controlled study, second year follow-up) clinical trial. Participants were randomized into ...

ea0081p185 | Reproductive and Developmental Endocrinology | ECE2022

Management of 46, XY 17 alpha-hydroxylase deficiency: a case report

Bouca Bruno , Cascao Mariana , Fiuza Pedro , Amaral Sara , Bogalho Paula , Silva-Nunes Jose

Introduction: 17 alpha-hydroxylase deficiency (17OHD) is a rare autosomal recessive disease caused by mutations in the CYP17A gene, representing 1% of cases of Congenital Adrenal Hyperplasia (CAH). The accumulation of mineralocorticoids and the glucocorticoid effect of corticosterone induce high blood pressure (HBP) and hypokalemia.Clinical Case: A 44 year-old female reporting HBP since the age of 20 years and without chronic medication, presented at the...

ea0081p186 | Reproductive and Developmental Endocrinology | ECE2022

Glycaemic and metabolic effects of testosterone replacement in hypogonadal men with uncontrolled type 2 diabetes − a randomised double blinded placebo controlled trial − stride study

Rao Preethi Mohan , Kelly Daniel M , Hugh Jones T.

The objective of the study was to assess the effect of intra-muscular testosterone on glycemic control, metabolic parameters and hypogonadal symptoms in men with hypogonadism and poorly controlled type 2 diabetes. This is a randomised double-blinded placebo-controlled add-on trial of intramuscular-testosterone undecanoate(Nebido®) administered every 12 weeks in 65 hypogonadal men with poorly-controlled diabetes. Phase-1 patients were randomly assigned to either...

ea0081p187 | Reproductive and Developmental Endocrinology | ECE2022

Overall impact of gender affirming hormone therapy: the portuguese experience

Saraiva Miguel , Santos Rafael , Figueiredo Zelia , Lemos Carolina , Palma Isabel

Introduction: The prevalence of Transgender individuals seeking gender affirming hormone therapy (GAHT) has been increasing. This therapy has been known to be highly effective in the treatment of gender dysphoria and enhancing mental health in this population.Aim: To evaluate the overall impact of GAHT on self-esteem, well-being and social/familial relations in the Portuguese adult transgender populationMethods: Cross-sectional stu...

ea0081p188 | Reproductive and Developmental Endocrinology | ECE2022

The effect of testosterone on quality of life, constitutional symptoms, sexual function and memory − randomised placebo-controlled study in hypogonadal men with uncontrolled type 2 diabetes − stride study

Rao Preethi Mohan , Hugh Jones T.

The objective of the study was to assess the effect of intra-muscular testosterone on constitutional symptoms, sexual symptoms, memory and in men with hypogonadism and poorly-controlled type-2 diabetes. This is a randomised double-blinded placebo-controlled add-on trial of intramuscular testosterone undecanoate(Nebido®) administered every 12 weeks in 65 hypogonadal men with poorly-controlled diabetes. Phase-1 patients were randomly assigned to either treatment ...

ea0081p189 | Reproductive and Developmental Endocrinology | ECE2022

Is there a link between polycystic ovary syndrome and transgenerational transmission of a reproductive and metabolic function in male offspring?

Risal Sanjiv , Li Congru , Luo Qing , Fornes Romina , Manti Maria , Lu Haojiang , Eriksson Gustaw , Ohlsson Claes , Lindgren Eva , Crisosto Nicolas , Maliqueo Manuel , Echiburu Barbara , Recabarren Sergio , Sir Petermann Teresa , Benrick Anna , Brusselaers Nele , Qiao Jie , Deng Qiaolin , Stener-Victorin Elisabet

Our previous study showed that polycystic ovary syndrome (PCOS)-like reproductive and metabolic phenotypes induced by maternal dihydrotestosterone (DHT)-exposure, can be passed on in mice from mothers (F0) to daughters (F1), granddaughters (F2), and even to great-granddaughters (F3). The female transmission is independent of diet-induced obesity and is mediated by transcriptional and mitochondrial perturbations of oocytes accompany. ...

ea0081p190 | Reproductive and Developmental Endocrinology | ECE2022

The role of B cells in immune cell activation in polycystic ovary syndrome

Ascani Angelo , Torstensson Sara , Risal Sanjiv , Lu Haojiang , Teschl Sabrina , Eriksson Gustaw , Li Congru , Menezes Joana , Sandor Katalin , Svensson Camilla , Stradner Martin , Obermayer-Pietsch Barbara , Stener-Victorin Elisabet

Objective: Age-associated double negative (DN) B memory cells lacking surface expression of CD27 and immunoglobulin D (IgD) are associated with proinflammatory characteristics and higher disease activity in autoimmune diseases. We first characterized B cells phenotypes in women with and without polycystic ovary syndrome (PCOS). We then took an in vivo approach, transferring purified IgG extracted from serum of hyperandrogenic women with PCOS to mice to establish wheth...

ea0081p191 | Reproductive and Developmental Endocrinology | ECE2022

FSH and bone: comparison between males with central vs primary hypogonadism

Giovanelli Luca , Cangiano Biagio , Colombo Stefano , Persani Luca , Quinton Richard , Bonomi Marco , Chiodini Iacopo

Background: Recent data suggest a direct effect of follicle stimulating hormone (FSH) on the skeletal metabolism. Particularly, it can encourage bone resorption and also inhibit osteoblast differentiation. High FSH levels have been found to correlate with impaired bone health in females, whilst evidence in males remains somewhat poor and conflicting. Intriguingly, men with primary and central hypogonadism might represent a novel study model in this context.<p class="abstex...

ea0081p192 | Reproductive and Developmental Endocrinology | ECE2022

Simulation via instant messaging − birmingham advance (SIMBA) as a tool to bridge gaps in clinical knowledge and expectations between physicians and patients with polycystic ovary syndrome

Sakthivel Pavithra , Melson Eka , Rezai Fatema , Synn Cuen Pan Carina , Sheikh Jameela , Kaur Harjeet , Cooper Catherine , Abdelhameed Farah , Pang Francesca , Bhatt Shreya , Shabbir Dania , Davitadze Meri , Gleeson Helena , Manolopoulos Konstantinos , Chu Justin , O' Reilly Michael , Arlt Wiebke , Gillett Caroline , Kempegowda Punith , Team Simba

Introduction: Polycystic Ovary Syndrome (PCOS) is the most common endocrinopathy in women. Studies exploring the experiences of people with PCOS reveal inadequate patient access to information and limited insight into healthcare professionals’ (HCP) attitudes regarding the condition. Simulation via Instant Messaging-Birmingham Advance (SIMBA) is a virtual simulation platform proven to increase learners’ confidence in their approach to simulated cases with a high acce...

ea0081p193 | Reproductive and Developmental Endocrinology | ECE2022

Efficacy of very low calorie ketogenic diet in obese PCOS: a randomized controlled study

Pandurevic Srdjan , Dionese Paola , Mancini Ilaria , Mitselman Dmitri , Magagnoli Matteo , Teglia Rita , Gazineo Domenica , Godino Lea , Fanelli Flaminia , Cristina Meriggiola Maria , Pagotto Uberto , Gambineri Alessandra

Background: Very low-calorie ketogenic diet (VLCKD) was shown to be effective in reducing weight and insulin resistance (IR) in obese patients. Considering that IR is very common in women with polycystic ovary syndrome (PCOS), and that IR worsens hyperandrogenism, ovulatory disfunction, and body fat accumulation, conceivably VLCKD could alleviate PCOS manifestations in the obese phenotype.Objective: This study compared the effects of a commercial VLCKD (...

ea0081p195 | Reproductive and Developmental Endocrinology | ECE2022

The effect of dihydrotestosterone on the immune profile in a mouse model of Polycystic Ovary Syndrome

Torstensson Sara , Ascani Angelo , Lu Haojiang , Risal Sanjiv , Eriksson Gustaw , Li Congru , Benrick Anna , Obermayer-Pietsch Barbara , Stener-Victorin Elisabet

Polycystic Ovary Syndrome (PCOS) is an endocrine disorder affecting 10-15% of women worldwide, characterized by high androgen levels, anovulation and/or polycystic ovarian morphology. Chronic low-grade inflammation is associated with the disorder as well as many of its comorbidities, such as obesity and type-2 diabetes. To determine the role of the immune system in the pathophysiology of PCOS, we characterized the immune profile of the dihydrotestosterone (DHT)-induced PCOS-li...

ea0081p196 | Reproductive and Developmental Endocrinology | ECE2022

Effects of octamethylcyclotetrasiloxane and decamethylcyclopentasiloxane on female reproductive systems in rat

Lee Jimin , Kim KangMin , Lee Minsu , Kim YongIn , Jeung Eui-Bae

In our daily life, humans are exposed to lots of chemicals. Among those chemicals, octamethylcyclotetrasiloxane (D4) and decamethylcyclopentasiloxane (D5) have been widely used in cosmetics for their functions such as softening, wetting, and viscosity control. However, the effect of these chemicals on the female reproductive systems has not been clearly elucidated. In the case of D4, it was recently recognized as an endocrine disrupting chemical (EDC), but the effect of D5 has...

ea0081p197 | Reproductive and Developmental Endocrinology | ECE2022

Functional analysis of anti-mullerian hormone variants in patients with polycystic ovary syndrome

Meng Li , McLuskey Anke , Visser Jenny

Objective: Recently rare heterozogous AMH genetic variants have been identified in women with polycystic ovary syndrome (PCOS) that result in reduced AMH signaling. However, the exact functional mechanism remains unknown. Therefore, we have performed functional analyses to analyze the processing, secretion and signaling of these PCOS-specific AMH rare variants.Methods: Six PCOS-specific AMH variants containing mutations (V12G, P151S, P270S, P352S, P362S,...

ea0081p198 | Reproductive and Developmental Endocrinology | ECE2022

Blue Morpho: An international survey investigating differences in emotional and psychosexual wellbeing by ethnicity and birthplace in women with polycystic ovary syndrome

Sheikh Jameela , Khalil Halimah , Shaikh Salomi , Hebbar Meghnaa , Zia Nawal , Wicks Saskia , Jayaprakash Sindoora , Subramanian Anuradhaa , Chapman Rachel , Gleeson Helena , Robinson Lynee , Chu Justin J. , Lathia Tejal , Selvan Chitra , O'Reilly Michael , Manolopoulos Konstantinos , Arlt Wiebke , Kempegowda Punith

Objective: This study investigated the association between ethnicity, birthplace and emotional and psychosexual wellbeing in women with Polycystic Ovary Syndrome (PCOS) in the community.Design: International cross-sectional study.Methods: Women with a self-reported PCOS diagnosis by a healthcare professional were invited to complete an online Blue Morpho questionnaire between September-October 2020 (UK) and May-June 2021 (India). F...

ea0081p199 | Reproductive and Developmental Endocrinology | ECE2022

Is N-terminal prohormone of B-type natriuretic peptide (NT-proBNP) disturbed in polycystic ovary syndrome?

Kałużna Małgorzata , Kompf Pola , Ziemnicka Katarzyna , Tomasz Krauze , Wachowiak-Ochmańska Katarzyna , Wykre¸towicz Andrzej , Guzik Przemyslaw , Ruchala Marek

Introduction: Polycystic ovary syndrome (PCOS) patients present with or without biochemical hyperandrogenism (HAPCOS or non-HAPCOS, respectively). Although cardiometabolic and hormonal abnormalities have been reported in women with PCOS, particularly those with hypertension, direct comparisons between normotensive (blood pressure (BP) <140/90 mmHg) patients with HAPCOS and non-HAPCOS are scarce. Data on N-terminal prohormone of B-type natriuretic peptide (NT-proBNP) in PCO...

ea0081p438 | Reproductive and Developmental Endocrinology | ECE2022

Central precocious puberty developed after treatment of leydig cell tumor: case report

Hadjer Bouguerra , Amina Khellaf , Adimi Amel , Arbouche Zakia

Background: Leydig cell tumors (LCTs) are the most prevalent hormone-secreting testicular tumors, but overall, a rare testicular tumor subtype. Surgery is the main therapy with a favorable prognosis. Nevertheless, the development of central precocious puberty after surgery of the tumor has been observed on rare occasions.Case Report: An 8.5-year-old boy presented with symptoms of sexual precocity dating back to 4 years. He had pubic hair (P4), enlarged l...

ea0081p439 | Reproductive and Developmental Endocrinology | ECE2022

Coexistence of 46, XX testicular disorder of sex development and 11β-hydroxylase deficiency: In Vivo and In Vitro Studies

Sun Bang , Wang Xi , Mao Jiangfeng , Wu Xueyan , Nie Min

Background: 46, XX testicular disorder of sex development (DSD) and 11β-hydroxylase deficiency (11β-OHD) are two extremely rare types of disorder of sex development. No coexistence has been reported yet.Case Description: Here we reported the first patient diagnosed as the coexistence of 11β-OHD and 46, XX testicular DSD basing on clinical, biochemical, molecular, cytogenetic, and functional experiment findings. A 22-year-old male showed sm...

ea0081p440 | Reproductive and Developmental Endocrinology | ECE2022

Cardiometabolic risk factors and diabetes mellitus are related with the severity of menopausal symptoms in middle-aged women

Armeni Elena , Kopanos Stylianos , Verykouki Eleni , Augoulea Areti , Paschou Stavroula A. , Rizos Demetrios , Kaparos George , Eleftheriades Makarios , Haidich Anna-Bettina , Goulis Dimitrios , Vlahos Nikolaos , Lambrinoudaki Irene

Background: Both surgical and spontaneous menopause are associated with increased cardiovascular disease (CVD) risk. Recent evidence reported that clusters of menopausal symptoms are associated with accumulation of cardiometabolic risk factors. Climacteric symptoms are also known to be affected by lifestyle parameters, ethnicity, the geographical location as well as by the overall health status at the time of the menopausal transition.Aim: This study aim...

ea0081p441 | Reproductive and Developmental Endocrinology | ECE2022

Usefulness of routine assessment of free testosterone for the diagnosis of functional male hypogonadism

Facondo Paolo , Di Lodovico Elena , Chiara Pezzaioli Letizia , Cappelli Carlo , Ferlin Alberto , Delbarba Andrea

Objective: To investigate whether routine assessment of free testosterone improves the diagnostic accuracy of functional male hypogonadism.Methods: Total and free testosterone (calculated through SHBG assessment) were determined in 372 male patients (median age 44) referring to our department for sexual symptoms symptoms (188 patients) or infertility (184 men). Low total and free testosterone were defined as < 2.31 ng/ml and <63 pg/ml, respective...

ea0081p442 | Reproductive and Developmental Endocrinology | ECE2022

Energy deficit as a cause of transient male hypogonadotropic hypogonadism: a successful resolution of a primary infertility

Benido Silva Vania , Teresa Pereira Maria , Rossano Louro Nuno , Barreiro Marcia

Introduction: Caloric restriction combined with overtraining can result in a total body energy deficit, which in turn is associated with multiple deleterious endocrine consequences, including hypogonadotropic hypogonadism. This can be a reversible cause of primary infertility, but its occurrence in men is still poorly recognized.Case Report: We report a case of a 39-year-old male evaluated in an urology appointment for primary infertility. He had a histo...

ea0081p443 | Reproductive and Developmental Endocrinology | ECE2022

Y-chromosome disomy and sexual ambiguity

Hasni Yosra , Zarrouk Oumayma , Tilouche Samia , Elfekih Hamza , Farid Hayfa , Maaroufi Amel , Kacem Maha , Chaieb Molka Chadli , Ach Koussay

Introduction: Chromosomal abnormality 47, XYY, despite being present in approximately 1 in 1000 newborn boys, remains less known phenotypically and more than 85% of men are never diagnosed. Males with 47, XYY syndrome are described to be phenotypically normal. They present often a developmental delay, behavioral difficulties and learning disabilities that may be associated with accelerated growth rate and taller stature in adulthood. Endocrine disorders, especially pubertal de...

ea0081p444 | Reproductive and Developmental Endocrinology | ECE2022

Use of selective venous vein sampling in postmenopausal women with hyperandrogenism of unclear aetiology

Romero Gregori Ana , Gimenez Olga , Capel Ismael , Subias David , Marcouchi Saad , Costa Laura , Perendreu Juan , Berlanga Eugenio , Rigla Mercedes

Background: Hyperandrogenism is infrequent in menopause, being the most common cause benign pathologies. However, it can also be produced by an ovarian or adrenal tumour. Correct diagnosis and location can be challenging because small lesions may not be visible with modern imaging studies. We report a case of ovarian stromal hyperplasia where selective venous catheterization and hormonal sampling were performed to find the origin of excessive androgen production.<p class="...

ea0081p445 | Reproductive and Developmental Endocrinology | ECE2022

Kisspeptin improves sexual brain processing in women with low sexual desire

Thurston Layla , Hunjan Tia , Ertl Natalie , B Wall Matt , Mills Edouard , Suladze Sofiya , Patel Bijal , Alexander Emma , Muzi Beatrice , A Rabiner Eugenii , Bech Paul , Goldmeier David , Abbara Ali , Comninos Alexander , Dhillo Waljit

Introduction: Sexual desire is a key component of the sexual response model. Absence or deficiency of sexual desire can lead to marked distress or interpersonal difficulty, termed ‘hypoactive sexual desire disorder’ (HSDD). HSDD is the most common female sexual health complaint worldwide, affecting up to 10% of women. Despite its detrimental impact on psychological well-being and quality of life, treatment options are currently limited. The hormone kisspeptin is a ke...

ea0081p446 | Reproductive and Developmental Endocrinology | ECE2022

The effect of gastric sleeve resection on menstrual pattern and ovulation in premenopausal women with class III-IV obesity

Tatarchuk Tetiana , Todurov Ivan , Anagnostis Panagiotis , Tutchenko Tetiana , Pedachenko Natalia , Glamazda Marina , Koseii Natalia , Regeda Svetlana

Purpose: Bariatric surgery is very efficacious in treating severe obesity. However, its effect of menstruation and ovulation is currently unknown. The purpose of this study was to assess the effect of gastric sleeve resection (GSR) on menstrual pattern in women with stage III-IV obesity and ovulatory dysfunction compared with conventional management.Methods: This was a prospective, multicentre, non-randomized trial, in premenopausal women, who fulfilled ...

ea0081p447 | Reproductive and Developmental Endocrinology | ECE2022

Interplay between estrogen signaling and notch pathway in rodent Sertoli cells

Lustofin Sylwia , Kamińska Alicja , Brzoskwinia Małgorzata , Cyran Joanna , Bilińska Barbara , Hejmej Anna

Somatic cells of the seminiferous epithelium, called Sertoli cells, play a key role in germ cell development and maintenance of proper course of spermatogenesis. Although androgens are considered the main regulators of Sertoli cell activity, recent studies indicate that their metabolites, estrogens, also influence Sertoli cell function. Estrogens, act mainly through nuclear estrogen receptors α and β (ERα, ERβ), however non-classical signaling via ...

ea0081p448 | Reproductive and Developmental Endocrinology | ECE2022

Finasteride inhibits epinephrine synthesis in humans: implication for sexual dysfunction

Giatti Silvia , Diviccaro Silvia , Di Domizio Alessandro , Cioffi Lucia , Falvo Eva , Caruso Donatella , Contini Alessandro , Cosimo Melcangi Roberto

Finasteride is a 5alpha-reductase (5α-R) inhibitor used in clinics to treat androgen-dependent conditions, such as benign prostate hyperplasia and androgenetic alopecia (AGA). Its use has been associated with several adverse effects, including sexual complaints. However, to date, no hypothesis to explain such adverse effects has been proposed. This is a consequence of the still incomplete knowledge of the intricate network of motivational, psychological, and molecular inp...

ea0081p449 | Reproductive and Developmental Endocrinology | ECE2022

Global impact of PCOS awareness month: challenges and opportunities

Malhotra Kashish , Synn Cuen Pan Carina , Davitadze Meri , Manolopoulos Konstantinos , O'Reilly Michael , Arlt Wiebke , Kempegowda Punith

Background: September is celebrated as PCOS awareness month worldwide and is aimed at improving the lives of those with PCOS, promoting research, and strengthening advocacy efforts. While significant financial and human resources are invested in promotions during this month, to the best of our knowledge, there is no evidence to show the impact of this initiative. In our study, we evaluated the global digital impact of PCOS Awareness Month, tracking commonly used hashtags as su...

ea0081p450 | Reproductive and Developmental Endocrinology | ECE2022

Influence of polycystic ovary syndrome on in vitro fertilization and relationship with the Asn680Ser polymorphism in FSHR gene

Vieira Ines , Filipa Ferreira Ana , Carvalho Alexandra , Dias Conceicao , Fernandes Silvana , Rodrigues Dircea , Cortesao Paulo , Almeida Santos Teresa , Paiva Isabel

Introduction: Polycystic ovary syndrome (PCOS) is a frequent cause of infertility. Its influence on the results of in vitro fertilization (IVF) is controversial, and generally not isolated from the effect of obesity. A relationship between the FSH receptor (FSHR) polymorphism Asn680Ser and the risk and phenotype of PCOS has been studied with conflicting results.Objectives: To analyze the influence of obesity and PCOS on the gonadal axis...

ea0081p451 | Reproductive and Developmental Endocrinology | ECE2022

Association between basal androgen concentrations and number of follicles on the day of triggering final oocyte maturation in poor responders undergoing IVF - a prospective study

Bosdou Julia , Anagnostis Panagiotis , Savvaidou Despoina , Zepiridis Leonidas , Goulis Dimitrios , Grimbizis Grigorios , Kolibianakis Efstratios

Purpose: Androgens promote early follicular development and granulosa cell proliferation by augmenting follicle-stimulating hormone (FSH) receptor expression in granulosa cells. Several retrospective studies have evaluated the association between basal androgen concentrations and follicular development in women undergoing ovarian stimulation for in vitro fertilization (IVF) with conflicting results. The aim of this study was to investigate whether basal androgen concentrations...

ea0081p452 | Reproductive and Developmental Endocrinology | ECE2022

A need for transgender care education among polish endocrinologists

Matwiej Katarzyna , Zawadzka Karolina , Sokolowski Grzegorz , Hubalewska-Dydejczyk Alicja , Trofimiuk-Muldner Malgorzata

Background: A significant body of research indicates that transgender and gender-nonconforming persons represent an underserved population susceptible to health care disparities. The attitudes and knowledge of medical doctors toward transgender people have important implications for the future quality of healthcare for transgender patients.Specific Aim: The aim of this study was an assessment of coverage of transgender care issues in training curricula o...

ea0081p453 | Reproductive and Developmental Endocrinology | ECE2022

Crosstalk between androgen-ZIP9 signalling and key regulators of mitochondrial dynamics in rodent leydig cells

Brzoskwinia Małgorzata , Kamińska Alicja , Lustofin Sylwia , Hejmej Anna , Bilińska Barbara

Androgen signalling plays a central role in the regulation of male reproduction. Androgens act predominantly by activating the classical intracellular androgen receptor (AR). In addition, alternative pathways may account for rapid effects of androgens via cytoplasmic or membrane-localized androgen receptor − ZIP9. In the testis, the main source of androgens, in particular testosterone, are Leydig cells. The proper synthesis of testosterone depends on the structural condi...

ea0081p454 | Reproductive and Developmental Endocrinology | ECE2022

Different grade of obesity influences the association between 25-hydroxyvitamin and Testosterone levels: A cross-sectional study in young Spanish men with Obesity

Boughanem Hatim , Damas-Fuentes Miguel , Vega Maria Molina , Jose Tinahones Madueno Francisco , Carlos Fernandez Garcia Jose , Macias Manuel

Backgrounds: One of the most observations in subjects with obesity, is a high prevalence of vitamin D deficiency, which, in turn, it has been implicated with decreased testosterone levels and an increased hypogonadism risk. Testosterone deficiency is a very common feature in males with obesity. However, only few studies have investigated the effect of vitamin D on testosterone in the context of obesity, and controversial results has been found.Hypothesis...

ea0081p455 | Reproductive and Developmental Endocrinology | ECE2022

Analysis of follicle-stimulating hormone receptor (FSHR)/g-protein coupled estrogen receptor (GPER) complex internalization through early and late endosomes

Lazzaretti Clara , Casadei Garofani Beatrice , Paradiso Elia , D'Alessandro Sara , Sperduti Samantha , Roy Neena , Mascolo Elisa , Baschieri Lara , Anzivino Claudia , Simoni Manuela , Casarini Livio

Introduction: Follicle-stimulating hormone (FSH) is a glycoprotein that support reproduction by regulating ovarian follicular growth and development. Recent studies demonstrated that proliferative signals mediating folliculogenesis are mediated by the G protein-coupled estrogen receptor (GPER) expressed in ovarian tissues throughout the follicular phase. In granulosa cells, GPER forms heteromers with FSHR, reprogramming cAMP-induced death signals to AKT-dependent, anti-apoptot...

ea0081p456 | Reproductive and Developmental Endocrinology | ECE2022

Common and unique transcriptional regulation in target tissues and oocytes across polycystic ovary syndrome-like mouse models

Deng Qiaolin , Pei Yu , Risal Sanjiv , Jiang Hong , Stener-Victorin Elisabet

Yu Pei and Sanjiv Risal are joint first authors. Elisabet Stener-Victorin and Qiaolin Deng are joint corresponding authors. Polycystic ovary syndrome (PCOS) affects around 15% of women of reproductive age and the key feature of the syndrome is hyperandrogenism. To understand the complex pathophysiology of PCOS, more than 30 PCOS-like animal models have been developed to mimic certain pathophysiological features. So far, not much is known about the common and unique molecular a...

ea0081p457 | Reproductive and Developmental Endocrinology | ECE2022

A systematic review and meta-analysis assessing psychosexual wellbeing in people with polycystic ovary syndrome

Khalil Halimah , Malhotra Kashish , Sheikh Jameela , Hebbar Meghnaa , Zia Nawal , Jayaprakash Sindoora , Wicks Saskia , Subramanian Anuradhaa , Chapman Rachel , Gleeson Helena , Robinson Lynne , Chu Justin , Lathia Tejal , Selvan Chitra , O'Reilly Michael W. , Manolopoulos Konstantinos , Arlt Wiebke , Kempegowda Punith

Background: Polycystic ovarian syndrome (PCOS) is a common endocrine disorder, with an estimated prevalence of 10-15% worldwide. PCOS can have adverse consequences on the emotional wellbeing of the patient. However, evidence exploring the relationship between PCOS and psychosexual wellbeing, specifically sexual function, and dysmorphic appearance concerns, is largely inconclusive.Aim: A systematic review and meta-analysis were undertaken to assess differ...

ea0081p695 | Reproductive and Developmental Endocrinology | ECE2022

Andrological effects of SARS-Cov2 infection: a systematic review and meta-analysis

Vena Walter , Corona Giovanni , Pizzocaro Alessandro , Pallotti Francesco , Paoli Donatella , Rastrelli Giulia , Baldi Elisabetta , Cilloni Nicola , Semeraro Federico , Salonia Andrea , Minhas Suks , Pivonello Rosario , Sforza Alessandra , Vignozzi Linda , Marcello Isidori Andrea , Lenzi Andrea , Maggi Mario , Lombardo Francesco

Background: Since the preliminary epidemiological data concerning the coronavirus disease 2019 (COVID-19) has been availabe, clear sex disparity has been evident, with males, although not more frequently affected, often experiencing worse outcomes when compared to women. The short and long term andrological effects of coronavirus disease 2019 (COVID-19) have not been clarified. The aim of the present study is to systematically review and meta-analyze all available data regardi...

ea0081p696 | Reproductive and Developmental Endocrinology | ECE2022

Comparison of three different AMH assays with AMH levels and follicle count in women with polycystic ovary syndrome

Moolhuijsen Loes , Louwers Yvonne , Laven Joop , Visser Jenny

Anti-Müllerian hormone (AMH) levels strongly correlate with the number of antral follicles in the ovary. In women with polycystic ovary syndrome (PCOS), this is reflected by significantly increased serum AMH levels. AMH levels are therefore suggested as a proxy for polycystic ovary morphology (PCOM) in PCOS diagnosis. Different assays are available to measure serum AMH levels. However, lack of a golden standard and the use of different antibodies to detect AMH, have led t...

ea0081p697 | Reproductive and Developmental Endocrinology | ECE2022

Clinical/biochemical characteristics of functioning gonadotroph adenomas in women presenting with ovarian hyperstimulation

Papanikolaou Nikoleta , Coulden Amy , Parker Nina , Lee Sit , Kelly Chris , Anderson Richard , Rees Aled , Cox Jeremy , Dhillo Waljit , Meeran Karim , Al-Memar Maya , Karavitaki Niki , Jayasena Channa

Background: Functioning gonadotroph adenomas (FGA) are rare benign pituitary tumours. Several case reports suggest that FGA may present with features of ovarian hyperstimulation in women. However, a lack of aggregated clinical experience of FGA precludes the meaningful guidance of management in affected women.Methods: Case series of 7 women presenting at different UK sites with FGA induced ovarian hyperstimulation syndrome (OHSS).R...

ea0081p698 | Reproductive and Developmental Endocrinology | ECE2022

The biochemical investigation of PCOS: a UK wide survey of laboratory practice

M Hawley James , Marrington Rachel , MacKenzie Finlay , Adaway Jo , Taylor Angela , Arlt Wiebke , Keevil Brian

Background: Polycystic ovary syndrome (PCOS) is a heterogenous condition that affects approximately 12% of females. The diagnosis can be complex and biochemistry tests are routinely relied upon to help identify hyperandrogenaemia and to exclude other conditions. However, although national and international guidelines advocate the use biochemistry tests, little information is provided as to which tests should be used and which other endocrinopathies should be excluded.<p cl...

ea0081p699 | Reproductive and Developmental Endocrinology | ECE2022

Cyproterone associated meningioma in a polycystic ovarian syndrome patient- a rare occurrence in our cohort

Jumaah Ali Al , Reddy Narendra , J Levy Miles , Bhake Ragini , Gohil Shailesh

Introduction: Meningiomas are the most common brain tumours and they express progesterone receptors. Cyproterone acetate (CPA) is a synthetic progestogen approved for use as an anti-androgen in Polycystic Ovarian Syndrome (PCOS). We report a case of meningioma probably resulting from long-term CPA treatment and a review of our PCOS cohort for further cases.Case report: A 47-year-old lady with PCOS was treated with CPA 100 mg once daily for hirsutism and ...

ea0081p700 | Reproductive and Developmental Endocrinology | ECE2022

Fetal exposure to anti-müllerian hormone triggers a transgenerational epigenetic transmission of polycystic ovary syndrome (PCOS) defects in adulthood

Nour El Houda Mimouni , Paiva Isabel , Barbotin Anne-Laure , Ezzahra Timzoura Fatima , Plassard Damien , Le Gras Stephanie , Ternier Gaetan , Pigny Pascal , Catteau-Jonard Sophie , Simon Virginie , Prevot Vincent , Boutillier Anne-Laurence , Giacobini Paolo

Polycystic ovary syndrome (PCOS) is the most common endocrine and metabolic disorder affecting women in reproductive age. Women with PCOS exhibit 2-3x higher levels of circulating Anti-Müllerian Hormone (AMH) as compared to healthy women and it is unclear if the elevation of AMH is a bystander effect or is driving the condition. Moreover, PCOS has a strong heritable component, however the etiopathology of the disease and the mechanisms underlying its transmission remain t...

ea0081p701 | Reproductive and Developmental Endocrinology | ECE2022

Evolution of congenital hypothyroidism with in situ thyroid gland in children and adolescents

Pignata Luisa , Bagattini Brunella , Montanelli Lucia , Agretti Patrizia , De Marco Giuseppina , Ferrarini Eleonora , Orsolini Francesca , Tonacchera Massimo

Background: In recent years, increasing cases of congenital hypothyroidism (CH) with in situ thyroid gland are identified. Outcome of children affected from CH with normally sited thyroid of normal size is still unknown. The objective of our study is to describe the natural history of this specific form of CH. Patients and methods: We retrospectively evaluated clinical, biochemical and instrumental data of 74 patients with diagnosis of CH with in situ thyroid...

ea0081p702 | Reproductive and Developmental Endocrinology | ECE2022

Non-alcoholic fatty liver disease prevalence in Klinefelter syndrome

Digitale Selvaggio Lucia , Di Fraia Rosa , Allosso Francesca , Marrone Aldo , Pasquali Daniela

Non-alcoholic fatty liver disease (NAFLD) is becoming more common over the world. Its predisposition for evolving to cirrhosis and hepatocellular cancer, as well as its link to extrahepatic symptoms, puts patients and clinicians under a double burden. Several studies have found a link between NAFLD and many endocrinopathies, demonstrating a substantial bi-directional link between NAFLD and hypogonadism, in both men and women. In man with T2DM, NAFLD is linked to reduced total ...

ea0081p703 | Reproductive and Developmental Endocrinology | ECE2022

Early changes in androgen hormones in individuals with spinal cord injury: a longitudinal study

Adam Itodo Oche , Francis Raguindin Peter , Frankl Gion , Wollner Jens , Eriks-Hoogland Inge , Jordan Xavier , Hund-Georgiadis Margret , Muka Taulant , Pannek Jurgen , Stoyanov Jivko , Glisic Marija

Background: Individuals with spinal cord injury (SCI) are in increased risk of hypothalamic−pituitary−gonadal axis disruption. We aimed to explore changes in androgen hormones during first impatient rehabilitation and identify factors associated with their levels among participants from the Swiss Spinal Cord Injury Cohort (SwiSCI) cohort.Methods: We measured sex hormones using Enyzyme Linked Immunosorbent Assay in persons with a newly aquired...

ea0081p704 | Reproductive and Developmental Endocrinology | ECE2022

Intracranial idiopathic hypertension in a maletranssexual patient after testosterone overdosing

Javier Martinez Martin Francisco , Hernandez-Lazaro Alba , de Leon-Durango Ricardo , Kuzior Agnieszka , Gonzalez-Diaz Paula , Perdomo-Herrera Esperanza , Lucia Tocino-Hernandez Alba , Arnas-Leon Claudia , Acosta-Calero Carmen , del Pino Perez-Garcia Maria

Introduction: Intracranial Idiopathic Hypertension (IIH) is a rare complication of testosterone therapy. It is usually benign, but may result and permanent blindness. Its diagnosis is based on a high CSF (> 25 cm H2O) pressure, in the absence of specific causes. The androgen receptor is expressed in the human choroid plexus, and may enhance the activity of Na+/K+-ATPase, and therefore CSF secretion.Methods: Review of the patient’s cli...

ea0081p705 | Reproductive and Developmental Endocrinology | ECE2022

Lessons from a patient with the 48XXXY karyotype: Not just another case of Klinefelter’s syndrome

Kuzior Agnieszka , Hernandez-Lazaro Alba , de Leon-Durango Ricardo , Rios-Gomez Carlos , Arnas-Leon Claudia , Acosta-Calero Carmen , Maria Fernandez-Trujillo-Comenge Paula , Delia Santana-Suarez Ana , Gonzalez-Diaz Paula , Javier Martinez Martin Francisco

Introduction: The 48XXXY karyotype is an infrequent (incidence about 1/50000 male births) sporadic aneuploidy of the sex chromosomes, classically considered as a variant of the Klinefelter syndrome (47XXY). Although many of their characteristics are shared, patients with the 48XXXY karyotype suffer from additional endocrinological and neuropsychological disturbances which are not part of the classic Klinefelter syndrome. Hereby we present a clinical case.<p class="abstext"...

ea0081p706 | Reproductive and Developmental Endocrinology | ECE2022

Alterations in lipid composition are linked with decreased motility in human spermatozoa

Guerra-Carvalho Barbara , G Alves Marco , Pinto Soraia , Barros Alberto , Ferreira Rita , A Carvalho Rui , Domingues Pedro , F Oliveira Pedro

Infertility is a growing concern in Western countries. Several factors, including lifestyle habits and increased prevalence of chronic disorders associated with hormonal alterations, increased chronic inflammation and systemic oxidative stress (such as obesity and diabetes mellitus), are contributing to the reduction of reproductive potential among males in modern societies. Evidence suggests these factors negatively impact human sperm quality resulting in a combination of alt...

ea0081p707 | Reproductive and Developmental Endocrinology | ECE2022

The role of hypothalamic markers in the development of POI following COVID-19

Baqoyeva Nilufar , Xaydarova Feruza

Introduction: Premature ovarian insufficiency (POI) is a pathological condition, which accompanying with loss of ovarian function in women under the age of 40. This is manifested by hypergonadotropic amenorrhea, infertility and estrogen deficiency. POI affects 1% of women. The specific causes can be genetic disorders, autoimmune, infectious-toxic and iatrogenic factors, but the exact mechanism is unclear yet. According to different authors, the idiopathic form accounts for 50 ...

ea0081p708 | Reproductive and Developmental Endocrinology | ECE2022

The medical treatment of polycystic ovary syndrome: effects of inositol isomers and metformin on clinical, metabolic and hormonal profile

Verde Nunzia , Pellegrini Bianca , Auriemma Lucia , Baratto Alessia , De Angelis Cristina , Garifalos Francesco , Menafra Davide , Castoro Michele , Simeoli Chiara , Colao Annamaria , Pivonello Rosario , Simona Auriemma Renata

Polycystic ovary syndrome (PCOS) is characterized by menstrual irregularities and clinical and biochemical hyperandrogenism, and is associated with insulin resistance, visceral obesity and metabolic disorders. The aim of the current study was to compare the effects of inositol isomers (INO) and metformin (MET) on the clinical, metabolic and hormonal aspects of PCOS. In 94 PCOS women, clinical (weight, BMI, waist circumference - WC, menstrual intervals, Ferriman-Gallway score -...

ea0081p709 | Reproductive and Developmental Endocrinology | ECE2022

Serum testosterone mirrors inflammation parameteres in females admitted with covid-19 disease

Francesca Birtolo Maria , Vena Walter , Pizzocaro Alessandro , Jaafar Simona , Ciafardini Antea , Brunetti Alessandro , Pigni Stella , Mazziotti Gherardo , Lania Andrea

Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is showing a rapid and continuous evolution in terms of new waves, the spread of variants and the evidence of reinfections. The growing heterogeneity of clinical presentation requires the identification of underlying pathogenic mechanisms to allow a better risk stratification. Previous studies analysed the role of sex hormones in disease severity demonstrating in male patients the association of...

ea0081p710 | Reproductive and Developmental Endocrinology | ECE2022

Age- and body mass index-adjusted association between insulin sensitivity and risk factors for cardiovascular disease in polycystic ovary syndrome

Kałużna Małgorzata , Krauze Tomasz , Kompf Pola , Ziemnicka Katarzyna , Andrzej Wykretowicz , Ruchala Marek , Guzik Przemyslaw

Introduction: Impaired insulin sensitivity accompanies polycystic ovary syndrome (PCOS). Women with PCOS are usually at risk of premature cardiovascular disease, which increases with age and body weight. We studied the link between insulin sensitivity measured by the Matsuda Insulin Sensitivity Index (MISI) adjusted to body mass index (BMI) and age in otherwise healthy PCOS women.Methods: 250 adult women with PCOS of reproductive age (18-43 years old) un...

ea0081p711 | Reproductive and Developmental Endocrinology | ECE2022

Demographic trends and morbidity in the basque country transgender population (2009-2020)

Gonzalez Fernandez Pedro , del Carmen Fernandez Lopez Maria , Ponce de Leon Saenz de Navarrete Susana , Bolado Maria Gema Grau , Luisa Guadilla Fernandez Maria , Rica Echevarria Itxaso

Introduction: The number of transgender men and women who seek gender-affirming therapy is rapidly increasing worldwide. It is still a population at high risk for several morbidities. The Basque Country Gender Identity Clinic was created in 2009 to centralize their medical care in the region.Aim: To determine the proportion of transgender individuals in the Basque Country, the prevalence of several morbidities in this transgender population, their evolut...

ea0081p712 | Reproductive and Developmental Endocrinology | ECE2022

Phenotypic and genotypic heterogeneity of sexual development disorders 46, XY in the Tunisian population

Haj Kacem Akid Faten , Abdellahi Mohamed Ahmed Mohamed , Nessrine Dhieb , B Benrhouma , T Kammoun , M Hachicha , N Kallel , N Belguith , Mnif Mouna , Abid Mohamed

Introduction: Sexual disorders 46 XY DSD are responsible for a range of phenotypic disorders, ranging from an ambiguous phenotype to a complete female phenotype. In this context, we report a cohort of 22 46 XY patients with a female phenotype in order to establish a phenotype-genotype correlation.Results: The average age at diagnosis was 15.5 years (E: 7 days-33 years). The reason for consultation was primary amenorrhea in 16 cases (72.7%), sexual ambigu...

ea0081p713 | Reproductive and Developmental Endocrinology | ECE2022

Molecular and functional studies of novel genetic variants of TP63 an SAMD11 genes unravel their potential role in the pathogenesis of primary ovarian insufficiency

Rossetti Raffaella , Moleri Silvia , Guizzardi Fabiana , Bonomi Marco , Marozzi Anna , Brancati Francesco , Persani Luca

Primary ovarian insufficiency (POI) is one of the major causes of female infertility, affecting about 3.7% of women before the age of 40. POI is associated with the premature loss of ovarian function and can manifest with primary amenorrhea (PA) or post-pubertal secondary amenorrhea (SA), with elevated gonadotropins and hypoestrogenism. Several evidence established a clear genetic component to POI, albeit heterogeneous. In search of novel causative genes, we screened 64 POI pa...

ea0081p714 | Reproductive and Developmental Endocrinology | ECE2022

Serum concentrations of 17-hydroxyprogesterone and dehydroepiandrosterone sulfate in women with polycystic ovary syndrome and their relation to the parameters of glucose metabolism

Stogowska Ewa , Krentowska Anna , Adamska Agnieszka , Lebkowska Agnieszka , Kowalska Irina

Introduction: Polycystic ovary syndrome (PCOS) is characterized by menstrual disorders, hyperandrogenism and polycystic ovaries on ultrasonography. This hormonal disorder is also strongly associated with insulin resistance. The diagnosis of PCOS can be established after the exclusion of other causes of mentioned symptoms, such as non-classic congenital adrenal hyperplasia (NCAH). To date, several studies suggest the increased serum concentration of 17-hydroxyprogesterone (17-O...

ea0081p715 | Reproductive and Developmental Endocrinology | ECE2022

Early career clinicians’ knowledge about lifestyle management of PCOS and the current practices of implementing it in clinical practice - does more need to be done?

Zia Nawal , Sheikh Jameela , Khalil Halimah , Narendran Alisha , Hebbar Meghnaa , Wicks Saskia , Jayaprakash Sindoora , Kempegowda Punith , SEva Team Pcos

Objective: Polycystic Ovary Syndrome (PCOS) is one of the most common endocrine conditions affecting women of reproductive age. Current estimates place the condition as more common than Type 2 Diabetes Mellitus (T2DM). Recent studies have shown several long term comorbidities associated with PCOS, thus making it essential that all physicians, regardless of training and speciality, understand and empower women with PCOS to adopt evidence-based behavioural changes. This study se...