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45th Annual Meeting of the European Thyroid Association (ETA) 2023

Poster Presentations

Translational 2

ea0092ps3-28-01 | Translational 2 | ETA2023

Study on the role of podoplanin in papillary thyroid carcinoma using rna sequencing

Gawel Damian , Mielecki Damian , Bętkowska Anna , Rozwadowski Marcin , Gawel Agata M. , Zeber-Lubecka Natalia , Kulecka Maria , Gajda Ewa , Godlewska Marlena

Introduction: Podoplanin (PDPN) is a transmembrane protein linked with metastases of various cancers, including papillary thyroid carcinoma (PTC). PDPN is significantly upregulated in PTC specimens and PTC- derived cell lines: TPC1 and BCPAP, harboring RET/PTC and BRAF V600E alterations, respectively. We observed that depletion of PDPN impairs migration of TPC1 cells, but surprisingly, augments metastases of BCPAP cells. To elucidate this phenomenon we aimed to further investi...

ea0092ps3-28-02 | Translational 2 | ETA2023

Hypothesizing a role of ret hyperactivation on weight control in patients with type 2A multiple endocrine neoplasia (MEN2A)

Prete Alessandro , Gambale Carla , Piaggi Paolo , Romei Cristina , Cappagli Virginia , Bottici Valeria , Elisei Rossella , Matrone Antonio

Introduction: RET gene is responsible for various human cancers and is mutated and hyperactivated in nearly 100% of familial (germline mutation) and in about 60% of sporadic (somatic mutation) MTC cases. Moreover, RET gene play a role in several physiologic and pathologic conditions. Very recently, the hyperfunction of RET was found to be a key process in weight loss. The hypothesis is that in a peculiar population in which RET gene is mutated and constitutively activated from...

ea0092ps3-28-03 | Translational 2 | ETA2023

Molecular analysis of multiple foci of sporadic medullary thyroid carcinoma suggests that they are intrathyroidal metastases from the same primary tumor

Almeida Jacqueline , Ciampi Raffaele , Casalini Roberta , Ramone Teresa , Mori Sara , Cappagli Virginia , Materazzi Gabriele , Ugolini Clara , Basolo Fulvio , Ward Laura , Elisei Rossella , Romei Cristina

Sporadic medullary thyroid cancer (sMTC) presents mutations of the RET proto-oncogene in about 50% of the cases and of the RAS proto-oncogene in about 20% of the cases. In about 15% of cases, sMTC is multifocal and it is not known whether multiple foci can be considered as intrathyroidal metastases of the main tumor focus or if they can be considered as independent tumors. To clarify this issue, we examined the mutation profile of multiple intrathyroidal tumo...

ea0092ps3-28-04 | Translational 2 | ETA2023

Effect of pazopanib in anaplastic thyroid cancer in primary culture

Martina Ferrari Silvia , Elia Giusy , Ragusa Francesca , Rosaria Paparo Sabrina , Mazzi Valeria , Patrizio Armando , Piaggi Simona , Baldini Enke , Centanni Marco , La Motta Concettina , Antonelli Alessandro , Fallahi Poupak

Anaplastic thyroid cancer (ATC) is usually treated with surgery, external hyperfractionated radiation therapy, and chemotherapy. However, because of the aggressiveness of this type of cancer, these treatments allow about 6-10 months of median survival. Therefore, it is challenging to predict the ATC patient clinical therapy responsiveness. Pazopanib is a multitarget tyrosine kinase inhibitor of VEGF receptors, PDGF, and c-Kit, and its effect in primary human ATC cells (pATC) h...

ea0092ps3-28-05 | Translational 2 | ETA2023

Pediatric thyroid nodules with germline and somatic DICER1 variants

Mastnikova Karolina , Bulanova Barbora , Sykorova Vlasta , Vaclavikova Eliska , Moravcova Jitka , Katra Rami , Vlcek Petr , Kodetova Daniela , Procykova Kristyna , Vcelak Josef , Bendlova Bela

Objectives: Pathogenic variants in the DICER1 gene can be found in both benign and malignant thyroid nodules, more often in pediatric patients. Somatic pathogenic variants in "hotspot" regions can coexist with germline variants in other regions of the DICER1 gene, which are associated with DICER1 syndrome, an autosomal dominantly inherited disease that predisposes to the development of various tumors from childhood. In addition to thyroid tumors, pleu...

ea0092ps3-28-06 | Translational 2 | ETA2023

Next-generation-sequencing on fine needle aspirates in neck recurrence of thyroid cancers

Theodon Helene , Buffet Camille , Guillerm Erell , Wasserman Johanna , Deniziaut Gabrielle , Jaffrelot Loic , Denis Jerome , Chereau Nathalie , Bigorgne Claude , Potonnier Wiame , Coulet Florence , Leenhardt Laurence

Objectives: Tumor molecular genotyping plays a key role in improving the management of advanced thyroid cancers. Molecular tests are classically performed on Formalin-Fixed Paraffin-Embedded (FFPE) carcinoma tissue. However alternative molecular testing strategies are needed when FFPE tumoral tissue is unavailable. The objective of our study was to retrospectively assess the performance of targeted DNA and RNA-based Next Generation Sequencing (NGS) on the fine needle aspirate ...

ea0092ps3-28-07 | Translational 2 | ETA2023

Expression of focal adhesion kinase microrna regulators is related to dedifferentiation in thyroid neoplasia spectrum

Ignjatović Jocić Valentina , Janković Miljuš Jelena , Išić Denčić Tijana , Živaljević Vladan , Tatić Svetilsav , Đorić Ilona , Selemetjev Sonja

Objectives: The specter of malignant thyroid lesions comprises tumors with different degrees of differentiation, rendering the differential diagnosis and prognosis of these tumors challenging. Focal adhesion kinase (FAK) is a tyrosine kinase involved in cellular communication and locomotion, functioning as a hub in the interactome of focal adhesions. Its upregulation has been demonstrated in a number of tumor types including thyroid cancer. FAK is regulated on several levels, ...

ea0092ps3-28-08 | Translational 2 | ETA2023

Understanding the putative role OF ACE2, TMPRSS2 and furin proteins on thyroid neoplastic transformation

Teresa Azevedo Maria , Macedo Sofia , Canberk Sule , Batista Rui , Soares Paula

Angiotensin-converting enzyme 2 (ACE2), Transmembrane serine protease 2 (TMPRSS2) and Furin were known to be key players for the SARS-CoV-2 infection. Thyroid gland was shown to be one of the relevant targets for SARS-CoV-2 infection allegedly due to the higher expression levels of these molecules in follicular cells. However, it remains to be fully determined the expression of ACE2, TMPRSS2 and Furin in normal follicular cell. In addition, the putative role of these molecules...

ea0092ps3-28-09 | Translational 2 | ETA2023

Detection of ras mutations leads to non-aggressive treatment of thyroid tumors

Tumasyan Dalar , Khroyan Armine , Muradyan Irina , Aghajanova Elena

Introduction: Noninvasive follicular thyroid neoplasm with papillary-like nuclear features (NIFTP) is an encapsulated or well-defined, not invasive neoplasm with a follicular growth pattern and core features of papillary thyroid carcinoma (PTC). This is considered to be a ’pre-malignant’ lesion of the RAS-like group. The RAS oncogene plays a huge role in human tumorigenesis. RAS gene mutations occur, in an average of 30–45% of follicular thyroid cancers (FTC). A...