SFEBES2023 Poster Presentations Metabolism, Obesity and Diabetes (70 abstracts)
Super-resolution ultrasound imaging approaches to visualise changes in gut structure
Cecilia Dunsterville 1 , Clotilde Vie 1 , Jacob Broughton Venner 1 , Jipeng Yan 1 , Alastair JH Brown 2 , Meng-Xing Tang 1 & Kevin G Murphy 1
1Imperial College London, London, United Kingdom. 2Sosei Heptares, Cambridge, United Kingdom
The gut plays an important role in energy homeostasis, and gut function is under intensive neuroendocrine control. The non-invasive imaging techniques contrast enhanced ultrasound (CEUS) imaging and super resolution ultrasound (SRUS) have the potential visualise the gut and provide novel insight into its structure and function. We aimed to demonstrate that changes in duodenal villi structure can be monitored using CEUS and SRUS, providing an insight into the structural of the gut. Rats (n=8) received twice daily vehicle control or the GLP-2 analogue teduglutide (0.3mg/kg; sc), known to drive small intestinal growth, for six days. In a separate experiment, rats (n=5) received once daily vehicle control or the gut-damage inducing drug methotrexate (2.5mg/kg; sc) for three days. Animals were imaged before and after using a high-frequency L22-14Vx probe (Verasonics, Kirkland WA). Following data processing, villi length increased by 22% by day 6 of the teduglutide treatment compared to a 9% decrease in the control group. Villi length was found to have decreased by 12.0% (SEM=4%) following MTX treatment, while the controls showed no significant change (2.8%; SEM=2.6%) from baseline. These results were in line with histology. This work demonstrates that SRUS can track structural changes in the duodenum and has potential, as a tool, to longitudinally study the gut and its role in homeostasis.
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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