Endocrine Abstracts

Background: Drosophila Melanogaster is a prominent animal model due to its short life cycle, ease of culture, and genetic makeup similar to that of humans. Acrylamide (ACR) is a naturally occurring, widely used chemical that is generated during high-temperature carbohydrate-rich meal. Significant doses of ACR have been associated with a variety of health problems, including neurotoxicity, reproductive toxicity, and immunotoxicity. Garlic (Allium Sativum) is an ancient medicinal plant, is used for disease treatment and spice worldwide due to its unique aroma.

Aim: This is to study ameliorative effect of Allium sativum on acrylamide-induced neurotoxicity in drosophila melanogaster model.

Methodology: Flies were divided into four groups; each with three vials and fifty flies. The three vials per group were to have a balanced and also variable data for the experiment. Control group fed with normal meal (CT), treatment group TGA fed with (200mg/kg of allium sativum), toxicant group TOA fed with (50mg/kg of acrylamide), treatment and toxicant group GAC fed with (200mg/kg of allium sativum + 50mg/kg of acrylamide). Behavioural assays and biochemical assays were then checked across all groups. GraphPad Prism statistical software package version 9.0, One-way ANOVA followed by post hoc ‘’Turkey’’ test were used for data analysis

Result: Acrylamide exposure significantly reduced flies’ life span, muscular integrity, locomotory, exploratory activities, total thiol, catalase activity, and protein levels; garlic intervention improved these aspects (P<0.05). The study shows increased MDA activity and AChe in the acrylamide group TOA, while co-administration of garlic extract lead to a significant reduction in MDA and AChe activity.

Conclusion: According to the findings of this study, Allium sativum & antioxidative properties improve flies’ life span, behavioural deficits and oxidative stress.

Key Words: Drosophila melanogaster, Allium sativa, Acrylamide, Behavioural assay.