Endocrine Abstracts

Objective: To investigate the relationship between endometriosis and Hashimoto’s thyroiditis and analyze their potential molecular mechanisms.

Methods: 1. Mendelian Randomization Analysis: Utilized GWAS data to assess the causal relationship between endometriosis and Hashimoto’s thyroiditis. 2. mRNA Expression Analysis: Analyzed mRNA expression patterns in endometriosis and Hashimoto’s thyroiditis tissues from the GEO database, including differential gene expression analysis, functional enrichment analysis, and pathway analysis. 3. Gene Regulatory Network Analysis: Constructed regulatory networks involving transcription factors (TFs), microRNAs (miRNAs), and target genes associated with highly expressed genes.

Results: 1. Mendelian Randomization Analysis: No direct causal relationship was found between endometriosis and Hashimoto’s thyroiditis. 2. mRNA Expression Analysis: Common upregulated and downregulated genes were identified in both diseases. Functional enrichment analysis showed that downregulated genes were associated with regulation of cell structure and function, while upregulated genes were associated with phosphorylation and deamination. Pathway analysis revealed significant enrichment of the NOTCH signaling pathway in both diseases. Friend analysis identified TNFRSF21 and MBNL3 as highly expressed genes in both diseases. 3. Gene Regulatory Network Analysis: Identified TFs and miRNAs that may regulate highly expressed genes. Constructed regulatory networks involving TFs, miRNAs, and target genes.

Conclusion: Despite the lack of a direct causal relationship established by Mendelian Randomization, significant overlap in mRNA expression patterns, cell signaling pathways, and immune regulation between endometriosis and Hashimoto’s thyroiditis suggests potential shared molecular mechanisms. Future research can further explore shared therapeutic targets for developing targeted treatment approaches.