Endocrine Abstracts

JOINT75

Polycystic Ovary Syndrome (PCOS) is a complex endocrine, reproductive, and metabolic disorder accompanied by hyperandrogenism and hyperinsulinemia mediated insulin resistance, but the precise molecular mechanism remains unclear. The study aimed to explore the potential link between RAB5B gene expressions and insulin resistant among women with PCOS.A total of age matched 270 subjects were enrolled in this study which included 135 PCOS women and 135 apparently healthy controls. The study participants were subjected to detailed medical history, clinical examination and physical examination. All subjects were further evaluated for biochemical, hormonal and inflammatory. Expression levels of RAB5B gene were analyzed using gene-specific primers and the SYBR® Green PCR Kit (Qiagen, Germany) and their qPCR reaction mix, according to the manufacturer’s guidelines. Student t-test and ANOVA were used to evaluate the differences in the means of various parameters. The HOMA-IR (2.28 ± 1.4 vs. 1.36± 0.73) was significantly elevated among women with PCOS than controls (p<0.001). We also found that the QUICKI (0.35±0.04 vs. 0.37±0.04), MATSUDA (12.59±4.71 vs. 15.47±4.33) and FGIR (11.56±7.06 vs. 14.32±8.66) were higher in controls than women with PCOS (P <0.001). Our findings revealed that women with PCOS exhibited significantly higher RAB5B mRNA levels compared to apparently healthy controls. This study highlights a potential link between RAB5B gene expression and insulin resistance, particularly in relation to insulin indices, in women with PCOS.

Keywords: Polycystic ovary syndrome, Insulin Resistance, HOMA-IR.