Endocrine Abstracts

JOINT3899

Introduction: Diabetes Mellitus (DM) is a chronic disorder of glucose metabolism with serious cardiovascular, endothelial, neurological consequences. In patients with DM, it appears that oxidative stress in the testicles is particularly high, which can cause significant impairment of testicular function including fertility or libido problems. Indeed, patients notice visible changes in testicular tissue as well as changes in hormonal balance.PurposeThe present research work aims to evaluate in vitro antioxidant activity in the seminal plasma of diabetic and non-diabetic individuals.Methods9 samples from non-diabetics and 6 samples from diabetic patients were collected (License from the Society for Ethics and Conduct No. 49947, 21/06/2024). Statistical analysis was performed using the SPSS statistical software v.29 (academic license). The total antioxidant capacity in seminal fluid was measured with FRAP method. The ferric-reducing antioxidant power (FRAP) assay involves reduction in Fe3+-2,4,6-tripyridyl-s-triazine (TPTZ) complex while taking absorbance at 593nm. The FRAP reagent is prepared by taking acetate buffer pH: 3.6, 10 mmol of TPTZ solution in 40 mmol of hydrochloric acid (HCl), and 20 mmol solution of iron (III) chloride in 10:1:1 (v/v) ratio. The standard curve was made with FeSO4.7 H2O and the results were expressed in μM FeSO4.7 H2O.

Results: Antioxidant activity in seminal fluid of diabetic was found statistically significant lower in diabetic compared to non diabetic men (1631,5 ± 151,7 μM FeSO4.7 H2O versus 3355,3 ± 640,1 μM FeSO4.7 H2O), p: <0,001.

Conclusions: Diabetes mellitus has a clear influence on antioxidant activity of the seminal plasma, since the normal values appear to be reduced, as already reported by other researchers.