Endocrine Abstracts

JOINT366

Background: HbA1c test provides information on metabolic control in diabetes and could also be used for its Diagnosis, it is essential to have accurate and precise HbA1c methods covering a range of measurement principles. We report an evaluation of the Biorad D10 kit (Hplc) versus Sebia capillarys 2 Kit (capillary electrophoresis).

Methods: Measurements of HbA1c were carried out in whole blood samples (K3edta tubes) from 111 patiens from different departments in Ziv medical Center- Israel using both Sebia Capillarys 2 Flex piercing (Capillary Electrophoresis) and analyzers Biorad D 10 (HPLC method).

Results: There was a good concordance between the results of capillary electrophoresis and HPLC (R2 = 0.97, P ⁢ 0.0001) There is no significant difference between the results obtained from both technique.

Conclusions: both technique suitable for the clinical application in the analysis of HbA1c. it is concluded that the results obtained after testing samples in Sebia capillarys Flex Piercing II and Biorad D10 are in a good concordance and there is no significant difference in the results obtained. The advantage of using Biorad D10 has benefit of shorter testing time Whereas Sebia capillarys can detecte underlying hemoglobinopathies and high throughput.

Biography: Medical lab scientist, BSc in medical lab science from Biology faculty in Technion, Haifa Israel. MSc in medical sciences (cancer research) from Medicine faculty in Technion, Haifa Israel. Working as a head of immunology lab in Ziv Medical Center, Israel.

Keywords: HbA1C, diabetes, capillarys, D10, diabetes diagnosis