Endocrine Abstracts

JOINT2269

Introduction: Oxidative stress shown as a main trigger factor of neurodegeneration in Altzheimer diseases (AD). We studied how antioxidant Liposomal-associated protein transmembrane 5 (PC5) can prevent brain neurodegeneration in rats models of AD by investigating behavioral activity.

Materials and methods: Experimental model of AD were created by intranasal injection of of AlCl₃ in dosage 50 mg/kg to adult rats during 7 days (AD group). Treatment group after 2h of each AlCl₃ injection received PC5 in dosage of 32 mg/kg were delivered in the same way (AD+PC5). Standard behavioral tests were performed in 3, 6, 9 days after treatment in AD, AD+PC5 and in control rats. Rats were sacrificed in the 10 days after experiment and tissue samples were taken flash frosen for further biochemical analysis.

Results: Rats with experimental AD presents with emotional and motosensor activity, postural instability, decreasing of walking distance, decreasing of quadrants crossing, decreasing of mink reflexes, also elongation of new labirints searching time. Decreasing of percentages of active and passive avoidance, also decreased learning coefficient for conditional reflexes. In brain tissue rats with AD content of peroxides were increased in 95%, activity of oxidative enzymes catalase, superoxide dismutase, glutation peroxide were decreased and malone dealdehyde were increased. In rats AD+PC5 group emotional and motosensor activity, quadrant crossing, new labirint searching time, also active and passive avoidance percentages were not differ from control group were not differ than in control group, except walking distance and mink reflexes. In brain tissue oxidative enzymes activity were higher, malone dealdehyde lower in compare with AD group.

Conclusion: Results suggested that PC5 significantly prevented AlCl_{3 induced} neurodegeneration in experimental AD rats evaluated by improving brain behavioural activity tests and oxidative stress enzymes activity.