ECEESPE2025 Poster Presentations Adrenal and Cardiovascular Endocrinology (169 abstracts)
Diurnal pattern of secretion of cortisol, Aldosterone and 18-hydroxycortisol levels in four biological fluids in healthy volunteers
Adelina Ameti 1 , Eder Zavala 2 , Ali Gilani 3 , Adamo Michela 1 , Vionnet Nathalie 1 , Raul Nicoli 4 , Nicolas Niederlander 1 , Paal Methlie 5 , Thomas Upton 6 , Adrian Ionescu 3 & Nelly Pitteloud 1
1Centre Hospitalier Vaudois CHUV, Service of Endocrinology, Diabetology and Metabolism, Lausanne, Switzerland, 2University of Birmingham, Department of Metabolism and Systems Science, Birmingham, UK, 3EPFL, Nanoelectronic Devices Laboratory, Lausanne, Switzerland, 4University of Lausanne, Swiss Laboratory for Doping Analyses, University Center of Legal Medicine, Lausanne, Switzerland, 5University of Bergen, Department of Clinical Science, Bergen, Norway, 6University of Bristol, Translational Health Sciences, Bristol, UK
JOINT3763
Background: Cortisol, aldosterone, and 18-hydroxycortisol are key adrenal steroids involved in metabolism, fluids homeostasis, and stress responses. Their secretion follows complex diurnal and ultradian rhythms. Due to their rhythmicity, adrenal hormones analysis following a single time-point measurement provides limited information on the state of the HPA activity and fails to inform on the nadir to peak concentrations.
Objectives: The primary objective of the study was to compare and correlate concentrations of cortisol, aldosterone and 18-hydroxycortisol in saliva, sweat and interstitial fluid to plasmatic levels,. The secondary objectives were to investigate the circadian rhythm of steroid profiles, including cortisol, aldosterone and 18-OH cortisol, in healthy volunteers and assess the influence of physical activity and dietary intake on these profiles.
Methods: A prospective study of 8 healthy volunteers with repeated sampling of blood, ISF, saliva and sweat during a 27-hour period was conducted.
1) 24 h adrenal steroids profiles in blood, interstitial fluid, saliva and sweat were measured by liquid chromatography–tandem mass spectrometry and analyzed.
2) Influence of a moderate bout of exercise and of meals on Free cortisol, 18-hydroxycortisol, and aldosterone variations were also assessed.
Results: Cortisol displayed a clear circadian rhythm, peaking in the early morning (0600–0700 h) and declining at night, with a strong correlation between serum and ISF. Aldosterone, known to be regulated by the renin–angiotensin system, showed an unexpected strong correlation with cortisol, suggesting a stronger influence of ACTH than previously described. Further, peak of Aldosterone were depicted after lunch and exercise. Saliva showed good correlations with serum for cortisol. Sweat analysis was unreliable for aldosterone and 18-hydroxycortisol due to low detectability but showed some correlation with serum cortisol levels.
Discussion: Our findings confirm ISF as a promising biofluid for real-time cortisol monitoring, given its correlation with serum variations despite slight temporal shifts. The data also challenge the conventional understanding of aldosterone regulation, highlighting a significant ACTH influence as well as the impact of meals and exercise on aldosterone secretion. Finally, these results underscore the limitations of single-point hormone measurements.
Volume 110
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Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
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