ECEESPE2025 Poster Presentations Endocrine Related Cancer (76 abstracts)
Genomic view of poorly diffentiated thyroid cancer as a bridge between well differentiated and anaplastic thyroid cancer
Sushmitha Sree Mekala 1 , Ramakanth Bhargav Panchangam 2 , Ramesh Bangaraiahgari 1 , Rajesh Bangaraiahgari 1 , Laxmi Narayana Sripuram 1 , Varshitha Eadula 1 , Amith Anshuli Dubey 1 , Kundana Sree K M 1 & Vidya Paboju 1
1TRR Institute of Medical Sciences, Hyderabad, India; 2BGH, Vijayawada, India
JOINT2331
Introduction: Poorly differentiated thyroid cancer (PDC) is a relatively infrequent form of differentiated thyroid cancer with intermediate prognosis between well differentiated (WDTC) and anaplastic cancers (ATC). But, this finding is debatable in literature. Genomics is the one of definitive modalities to clarify this moot point. ATC tends to express P53 and KMT2D mutations frequently, while WDTC have no such mutations. Similar Indian studies are scanty in PDC. In this context, we set out study the prevalence of these somatic mutations in surgical tissue samples of PDC.
Material and Methods: This prospective study was conducted on surgically managed thyroid cancer patients. Institutional ethical committee approval was obtained. Diagnosis was based on biochemical confirmation, imaging, fine needle aspiration cytology and later confirmed by histopathology. We selected 15 PDC, 4 ATC and 40 WDTC cases. Tumour tissue samples were taken from ex-vivo thyroidectomy specimen within operation theatre. After appropriate processing of samples, DNA extraction, cDNA preparation, PCR amplification, application of 2 sets of Primers were performed as part of mutational analysis of P53 and KMT2D genes.
Results: Heterozygous mutations in KMT2D gene and missense mutation in P53 gene were found in 4/15 (26. 6 %) and 7/15 (45%) of PDC cases respectively. In ATC, KMT2D mutations were seen in 3/4 (75%) and 4/4 (100%) cases. None of these gene mutations were seen WDTC cases.
Conclusions: Our study shows a distinct correlational pattern of mutations in KMT2D and P53 genes suggesting a causative linkage between the gene function and cancer cell differentiation. Further, PDC appears to be intermediate in mutation frequency, with ATC on higher side of scale. Though our results are not robust, it provides a platform for larger multi-institutional studies to justify this observation in future.
Key words: Thyroid cancer; Poorly differentiated cancer; Anaplasia; Follicular cell)
Volume 110
Article tools
My recent searches
My recently viewed abstracts
Authors
Endocrine Abstracts
ISSN 1470-3947 (print) | ISSN 1479-6848 (online)
© Bioscientifica 2025 |
Privacy policy |
Cookie settings
BiosciAbstracts
Bioscientifica Abstracts is the gateway to a series of products that provide a permanent, citable record of abstracts for biomedical and life science conferences.
Find out more