Searchable abstracts of presentations at key conferences in endocrinology

ea0077p102 | Reproductive Endocrinology | SFEBES2021

Differentially glycosylated FSHR ligands as potential modulators of FSHR quaternary complexes and FSHR-dependent signalling

Agwuegbo Uche , Colley Emily , Bousfield George , Albert Anthony , Jonas Kim

The G protein-coupled receptor, follicle-stimulating hormone receptor (FSHR), is essential for reproduction. A key drug target of IVF, understanding the mechanisms modulating FSHR functions remains of high importance. The endogenous ligand of the FSHR, FSH, is a heterodimeric glycoprotein hormone with two predominant glycoforms identified. Partially glycosylated FSH21 has faster binding kinetics to the FSHR and more potent at activating cAMP-dependent signal pathways, in compa...

ea0086op3.4 | Reproductive and Neuroendocrinology | SFEBES2022

Identification and characterisation of novel follicle-stimulating hormone receptor antagonists

Duyen Tran Hanh , Agwuegbo Uche , Albert Anthony , Jonas Kim

Follicle-stimulating hormone receptor (FSHR) is a Class A G protein-coupled receptor (GCPR) that is essential in reproduction. Interactions with its heterodimeric glycoprotein hormone, FSH, activates the cAMP/PKA signalling pathway, which induces steroidogenic activity and granulosa cell proliferation to support ovarian follicle growth and survival. Moreover, a number of ageing-related extragonadal roles of FSH/FSHR have been proposed, with menopausal elevation in FSH linked t...

ea0086p119 | Reproductive Endocrinology | SFEBES2022

FSH glycosylation variants differentially modulate FSHR trafficking

Agwuegbo Uche , Richardson Rachel , Albert Anthony , Hanyaloglu Aylin , Jonas Kim

The Class A G protein-coupled receptor (GPCR), follicle-stimulating hormone receptor (FSHR), and its associated heterodimeric glycoprotein hormone (FSH) are essential for reproduction. As such, they’re key targets of assisted conception. Post-translational modification of FSH gives rise to two predominant glycosylation variants, which are modulated with ageing: partially glycosylated FSH (FSH21/18), predominates in women’s reproductive prime (20’s), displays fas...

ea0065p348 | Reproductive Endocrinology and Biology | SFEBES2019

Impact of a FSHR positive allosteric modulator on FSH glycosylation variant-dependent FSHR homomerisation and signal pathway activation

Agwuegbo Uche , Albert Anthony , Bousfield George , Jonas Kim

The heterodimeric pituitary glycoprotein hormone, follicle-stimulating hormone (FSH) and its target G protein-coupled receptor (FSHR) are essential for reproduction. As an important drug target for IVF, the need for more effective treatment drives interest in understanding what modulates FSH/FSHR functions. In vivo, two predominant FSH glycosylation variants have been identified; partially glycosylated FSH (FSH21) has faster binding kinetics to the FSHR and is more potent at a...