Searchable abstracts of presentations at key conferences in endocrinology

ea0028ye1.4 | Obtaining funding and networking for young endocrinologists | SFEBES2012

The trials and tribulations of a University Lecturer

Fowkes Rob

Even before the current economic crisis, life in academia was already tough enough, with a distinct lack of career structure for basic scientists. Now that funding levels continue to plummet, a career in academia seems even less attractive. However, for many basic scientists, it is still their major aspiration; to gain a faculty position that allows them to pursue their research interests. I was hopelessly naive to what life as a University Lecturer was going to involve. Two d...

ea0015s69 | Obtaining funding for young endocrinologists | SFEBES2008

The Been-to-America degree revisited: is it really still worth it?

Fowkes Rob

Five years after my original post-doc experience overseas, I find myself increasingly contemplating the benefits of that time in my career and whether it was the right decision. Over that same period of time, it’s still clear to me that despite the introduction of a few more research fellowships for the privileged few, the career structure of basic scientists in the UK still appears to be virtually non-existent. So should you consider moving abroad?...

ea0015p262 | Pituitary | SFEBES2008

Transcriptional regulation of the human guanylyl cyclase-B (GC-B) promoter in gonadotroph and somatotroph cell lines

Chand Annisa , McGonnell Imelda , Fowkes Rob

Particulate guanylyl cyclases are expressed in all endocrine organs and serve as specific receptors of the natriuretic peptides ANP, BNP and CNP. Guanylyl cyclase-B (GC-B/Npr2) receptors specifically mediate the biological effects of CNP in its target tissues. Targeted disruption of either CNP or GC-B results in pituitary growth hormone deficiency and dwarfism as a result of achondroplasia. Despite this, molecular regulation of GC-B expression is poorly elucidated. Therefore, ...

ea0015p264 | Pituitary | SFEBES2008

Mechanism of C-type natriuretic peptide (CNP) stimulated phosphorylation of ERK-MAPK in pituitary GH3 somatotrophs

Jonas Kim , Thompson Iain , Fowkes Rob

CNP, the third peptide of the mammalian natriuretic peptide family, exerts its biological effects through activation of the guanylyl cyclase B (GC-B) receptor and resulting cGMP accumulation. Our previous studies reported that CNP stimulates phosphorylation of MAPK family proteins in GH3 somatotrophs. This present study aimed to further characterise the mechanism of CNP-stimulated ERK phosphorylation and resulting biological consequences in GH3 cells. Western blot analyses sho...

ea0015p269 | Pituitary | SFEBES2008

Homologous and heterologous desensitization of guanylyl cyclase-B (GC-B) receptor in GH3 somatotrophs: mechanisms to control the effects of C-type natriuretic peptide (CNP)

Thompson Iain , Wheeler-Jones Caroline , Fowkes Rob

Natriuretic peptides, ANP, BNP and CNP, exert the majority of their effects via particulate guanylyl cyclase receptors (GC-A or GC-B), resulting in increased cellular cGMP levels. Several studies have examined the pharmacological properties of GC-B receptors in many systems, and desensitization (tachyphylaxis, loss of response) has been reported to occur. Targeted deletion (in mice) or genetic mutations (in humans) of the GC-B receptor have been implicated in a dwarfism phenot...

ea0015p271 | Pituitary | SFEBES2008

Molecular and functional components of a gonadotroph natriuretic peptide system in α T3-1 and LβT2 cells

Thompson Iain , Wheeler-Jones Caroline , Fowkes Rob

Gonadotrophs have previously been shown to be the predominant source of C-type natriuretic peptide (CNP) in the anterior pituitary, and CNP causes enhanced cGMP accumulation in αT3-1 gonadotrophs. However, the biological role of CNP in gonadotrophs remains elusive. In the current study, we examined the molecular and functional characteristics of the gonadotroph natriuretic peptide system. Using two well-characterised gonadotroph cell lines of different developmental origi...

ea0015p296 | Reproduction | SFEBES2008

Hormonal regulation of 11β-hydroxysteroid dehydrogenase (11βHSD) enzyme activities in boar test is and caput epididymidis

Sharp Victoria , Michael Tony , Fowkes Rob

Cortisol is metabolised by two 11βHSD isoenzymes. Recently, we reported that 11βHSD1 and 11βHSD2 mRNA and protein are co-expressed in boar testis and throughout the male reproductive tract. However, enzyme activities were only detectable in boar testis, caput epididymidis, bulbourethral glands and penile urethra. The objective of the present study was to assess whether cortisol–cortisone metabolism in the boar testis and caput epididymidis could be regulate...

ea0031p293 | Pituitary | SFEBES2013

Pulsatile GnRH signaling to ERK: relevance of pulse duration and frequency

Perrett Rebecca , Armstrong Stephen , Fowkes Rob , McArdle Craig

GnRH is secreted in pulses and its effects on pituitary gonadotropes depend on pulse frequency. This is crucial for physiological control and therapeutic manipulation of the system (in IVF and treatment of hormone-dependent cancers) but GnRH pulse frequency decoding mechanisms are unknown. The simplest form of frequency dependence is a linear relationship between integrated inputs and outputs but such ‘integrative tracking’ cannot explain the bell-shaped frequency-re...

ea0028p280 | Reproduction | SFEBES2012

Relevance of ERK levels in acute gonadotrophin-releasing hormone (GnRH)-stimulated ERK activation

Perrett Rebecca , Caunt Christopher , Fowkes Rob , McArdle Craig

GnRH acts via G-protein coupled receptors to stimulate synthesis and secretion of luteinizing hormone and follicle-stimulating hormone. GnRH activates phospholipase C, causing calcium mobilisation and influx, activation of protein kinase C and stimulation of mitogen-activated protein kinase cascades. GnRH is secreted in brief pulses with each pulse causing rapid and transient activation of extracellular signal regulated kinases (ERKs) (1), yet very little is known about what s...

ea0013p245 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Phosphorylation of MAPK family proteins by C-type natriuretic peptide (CNP) in LbetaT2 gonadotrophs – a novel cyclic GMP-independent effect?

Jonas Kim , Thompson Iain , Chand Annisa , Fowkes Rob

CNP, the third member of the mammalian natriuretic peptide family, exerts the majority of its biological effects via the guanylyl cyclase B (GC-B) receptor, resulting in accumulation of intracellular cGMP. Previous studies reveal numerous inhibitory effects of natriuretic peptides on the MAPK family in bone, neuronal and cardiovascular tissues. However, we recently reported that CNP stimulates ERK, p38MAPK and JNK phosphorylation in pituitary GH3 somatotrophs. As CNP is known ...