Searchable abstracts of presentations at key conferences in endocrinology
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Society for Endocrinology BES

Poster Presentations

Neuroendocrinology and behaviour (including pituitary)

ea0013p226 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Annexin 1 interaction with calpain: a role in apoptosis

Peng Zhou , Morris John , Christian Helen

Annexin 1 (ANXA1) is a 37 Kd member of the annexin family of Ca2+− and phospholipid binding proteins that has been well demonstrated to act as a mediator of glucocorticoid action in inflammation and in the control of anterior pituitary hormone release. ANXA1 is also proapoptotic and has been identified as a mediator of apoptotic cell engulfment (Solito et al. 2003 FASEB J 17:1544; Arur et al. 2003 Dev Cell 4:587). Using yeast two hybrid screening...

ea0013p227 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Malignant prolactinoma presenting as nasal polyp

Kannappan Daniel , Amin Peshraw

67-year-old man re-attended A&E following minor head injury with disproportional symptoms of dizziness on sitting upright, vomiting and neck pain. He is also complaining of nasal blockage for past 1 year with anosmia and occasional yellow rhinorrhoea. On examination he has mild neck tenderness and cranial nerves were intact. Nasopharyncoscopy revealed fullness of posterior nasal space.Head CT was performed revealing extensive soft tissue tumour appea...

ea0013p228 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

The novel broad-spectrum somatostatin receptor agonist SOM230 (Pasireotide), blocks the adrenalectomy-induced increase in mitotic activity in male rat anterior pituitary

Nolan Lesley , Schmid Herbert , Levy Andrew

The novel somatostatin receptor agonist SOM230 (pasireotide) binds with high affinity to somatostatin receptors sst1, sst2, sst3 and sst5. Acting principally through the latter, it inhibits basal and CRH-stimulated ACTH secretion from the AtT20 mouse corticotroph cell line and ACTH release from a proportion of human corticotroph adenomas both in vitro and in vivo. Data suggesting an additional antiproliferative effect has led to SOM230 being explored as a potenti...

ea0013p229 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

The effects of radiotherapy on neurocognitive function in patients treated for non-functioning pituitary adenoma

Tooze Alana , Gittoes Neil , Jones Chris , Toogood Andrew

Background: Surgery, sometimes supported by adjunctive radiotherapy (RT) are the treatments of choice for patients with non-functioning pituitary adenomas (NFA). Previous studies have implicated these treatments in deficits of cognitive function, particularly memory and executive functioning, although this research has often been confounded by the inclusion of patients with hormone producing tumours. The aim of this study was to determine the impact of RT on psychometric funct...

ea0013p230 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

The AVP precursor, Copeptin, predicts the severity of acute and prolonged traumatic brain injury (TBI)

Kleindienst Andrea , Weigel Daniel , Morgenthaler Nils , Struck Joachim , Buchfelder Michael , Brabant Georg

Introduction: The incidence of water and electrolyte disturbances following traumatic brain injury (TBI) is considerable and has been attributed to a dysregulation of the hypothalamic peptide arginine-vasopressin (AVP). Copeptin, the C-terminal part of the AVP prohormone, is stoichiometrically secreted together with mature AVP. Due to his long ex vivo stability, the AVP precursor, copeptin, can be used as a surrogate of AVP readily to be measured in serum or plasma. In ...

ea0013p231 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

A prospective longitudinal study of anterior pituitary dysfunction following traumatic brain injury

Kleindienst Andrea , Weigel Daniel , Bock Christoph , Buchfelder Michael , Brabant Georg

Purpose: Neuroendocrine dysfunction represents a frequent consequence of traumatic brain injury (TBI) but has not been studied longitudinally as yet. Here we attempt to identify predictors of late endocrine insufficiency following TBI.Methods: 71 consecutive patients (age 18 – 87 yrs) with TBI assessed by an intial CT and the Glasgow Coma Score (GCS) were prospectively studied as to their pituitary function on day 0, 3 and 7 and dynamically 24–...

ea0013p232 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Relaxin-3 stimulates the stress axis

McGowan Barbara , Stanley Sarah , White Nick , Donovan Joe , Gardiner James , Ghatei Mohammad , Bloom Stephen

Relaxin-3 is a newly discovered member of the insulin superfamily. It is expressed in the nucleus incertus (NI) of the brainstem which has projections to the hypothalamus. Relaxin-3 binds with high affinity to the G-protein-coupled receptors RXFP1 and RXFP3. RXFP3 is expressed predominantly in the CNS, and in particular within the hypothalamic paraventricular nucleus (PVN). The physiological function of relaxin-3 is unknown but recent work suggests it may play a role in appeti...

ea0013p233 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Co-cultures of pituitary lactotrophs and folliculostellate cells stimulates connexin 43 expression: a novel role for the nucleoside adenosine

Lewis Barbara , Francis Karen , Pexa Annette , Deussen Andreas , Scanlon Maurice , Rees Daffyd , Ham Jack

Folliculostellate (FS) cells within the anterior pituitary gland produce cytokines and growth factors which are regulated by molecules produced by neighbouring endocrine cells. There are many factors that could potentially be responsible for this intercellular communication that is mediated via connexins (Cx) and gap-junctions. We thus investigated the effect of co-culturing lactotrophs (MMQ) and FS cells (TtT/GF) on Cx43 expression. Our in vitro model system consists o...

ea0013p234 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Effects of neurotensin and/or morphine on gene expression of mu and delta opioid receptor

Bai Bo , Liu Haiqing , Liu Yuhong

In the central nervous system, the opioid and non-opioid transmitter system jointly compete with multiple pathophysiological processes. The in vitro neuronal model of rat (Wistar) caudate putamen and reverse transcription-poly-merase chain reaction were used to examine the effects of neurotensin and/or morphine on the expression of mu or delt-opioid receptor mRNA. In the present study neurotensin (10 nmol/l) promoted but morphine (0.001 mmol/l) reduced mu or delt-opioid...

ea0013p235 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Growth hormone sensitivity in GH deficient (GHD) hypopituitary adults is dependent on gender, but independent of timing of onset

Columb Breeda , Jostel Andreas , Mukherjee Annice , Smethurst Linda , Shalet Stephen , Murray Robert

Females release 2–3 fold greater GH compared with males whilst maintaining similar IGF-I levels. IGF-I generation tests in healthy subjects suggest this discordancy results from resistance to GH in females. In GHD females the presumed relative insensitivity to GH is reflected by a lower basal IGF-I and higher GH maintenance doses during replacement. GHD adults of childhood- (CO) and adult-onset (AO) show CO-GHD adults to have lower basal IGF-I values and require higher ma...

ea0013p236 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

PYY3–36 produces an acute anorexigenic effect followed by a later orexigenic effect not seen with other anorexigenic peptides

Parkinson James , Dhillo Waljit , Small Caroline , Ghatei Mohammed , Bloom Stephen

PYY is secreted postprandially from the endocrine L-cells of the gastrointestinal tract. PYY3–36, the major circulating form of PYY, reduces food intake in humans and rodents via high affinity binding to the auto-inhibitory NPY receptor, Y2R, within the hypothalamic arcuate nucleus.Aims: To investigate (1) the effects of length of fast on the anorexigenic actions of PYY3–36; (2) the effects of early light phase administrat...

ea0013p237 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

A putative role for 11β-Hydroxysteroid dehydrogenase 1 in the regulation of cerebrospinal fluid dynamics

Sinclair Alexandra , Onyimba Claire , Khosla Pamela , Tomlinson Jeremy , Burdon Michael , Murray Philip , Stewart Paul , Walker Elizabeth , Rauz Saaeha

The pre-receptor regulation of glucocorticoids is mediated by 11β-hydroxysteroid dehydrogenases (11β-HSD). The type 1 isoform is primarily responsible for the generation of active cortisol in tissues such as liver and adipose, whereas 11β-HSD2 regulates sodium and ion transport. Recently, we have shown the presence of functional 11β-HSD1 in the ciliary body of the eye where it is important in control of sodium transport, aqueous humour production and intrao...

ea0013p238 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

The effect of exogenous estradiol administration on working memory in the ovariectomised female hooded-lister rat

Sutcliffe Jane , Neill Jo , Marshall Kay

The aim of the investigation was to evaluate the effect exogenous of 17-β-estradiol propionate (E2) on working memory as assessed by the novel object recognition (NOR) paradigm following intermittent or delayed E2 regimens.15 rats were ovariectomised under anaesthesia and were divided into 3 groups namely: (1) vehicle (2) intermittent E2 dosing weeks 1–6 and 13–18, with no treatment weeks 7–12 and (3) delayed (no treatment in weeks 1&...

ea0013p239 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

I am rare Turner mosaic – Can I have a baby?

Bennett Rachel , Chowdhury Sharmistha Roy , Page M.D.

Case History: A 16 yr old patient presented to us concerned about her short statue. Her father and mother were at the 9th centile and her sister was at the 25th centile in adult life. She was otherwise well having gone through a normal puberty in her early teens and was currently on the oral contraceptive pill having regular periods. On examination she did display some features of Turner’s syndrome; short stature, short neck and cubitus valgus deformity. Cytogenetic analy...

ea0013p240 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Traumatic brain injury and hypopituitarism

Rao-Balakrishna Prasanna , Dixit Kashinath , Sudagani Jaidev , Kearney Tara

Road traffic accidents resulting in traumatic brain injuries are increasingly common. Patients are often males in their third decade. Many of these patients persist to have various symptoms even after recovery from the acute phase, which tend to be diagnosed as post concussion syndromes. Interest has increased of late in Post traumatic hypopituitarism. We report two patients with head injuries with endocrine insufficiencies.A 21 year male was hit by a ca...

ea0013p241 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Late development of secondary resistance to cabergoline in a patient with a lactotroph adenoma

Draman Mohd Shazli , Moran Carla , King Tom , Crowley Rachel , O’Sullivan Eoin , Smith Diarmuid , Thompson Christopher , Agha Amar

Primary resistance to Cabergoline occurs in a minority of patients with lactotroph adenomas but the development of late resistance following initial response is an extremely rare phenomenon which has only been reported in very few cases.: We report the case of a 57 year old lady who was presented in 1997 with headaches and visual failure and was found to have a large pituitary mass with compression of the optic chiasm and invasion into the cavernous sinuses bilaterally. Serum ...

ea0013p242 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Subclinical hypothyroidism is associated with increased cardiovascular risk: A meta-analysis

Razvi Salman , Weaver Jolanta , Pearce Simon

There are discrepancies between different studies with regards to the association of subclinical hypothyroidism (SCH), dyslipidaemia and cardiovascular (CV) disease. Therefore, we have conducted a systematic search and meta-analysis of population-based studies of CV disease in which thyroid status was examined and randomised controlled trials (RCTs) of treatment.The PubMed and Embase databases were searched for relevant studies published between 1976 and...

ea0013p243 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Use of the dexamethasone-suppressed corticotrophin-releasing hormone test to predict cure in patients with cushing’s disease following pituitary surgery

Banerjee Anita , Martin Niamh , Dhillo Waljit , Roncaroli Federico , Meeran Karim

Establishing failure of trans-sphenoidal surgery (TSS) to cure Cushing’s disease in the immediate post-operative period is essential for early surgical re-exploration. However, there is no consensus regarding the definition of apparent cure. We studied whether the dexamethasone-suppressed corticotrophin-releasing hormone test (LDDST-CRH test) immediately following TSS could accurately identify those with Cushing’s disease requiring further definitive treatment.<p...

ea0013p244 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Non-surgical cerebro-spinal fluid rhinorrhea in invasive macroprolactinoma: incidence, radiological and clinicopathological features

Suliman SGI , Gurlek A , Byrne J , Sullivan N , Thanabalasingham G , Cudlip S , Ansorge O , Wass JAH

Objective: Macroprolactinomas (MPRL) may result in spontaneous or dopamine-agonist-induced CSF rhinorrhoea. The incidence of, and mechanisms underlying this phenomenon are poorly understood. In this study, we aimed to determine the incidence of non-surgical (spontaneous and dopamine-agonist-induced) rhinorrhoea, and to identify biochemical, radiological and histopathological factors associated with this phenomenon.Methods: We retrospectively reviewed MPR...

ea0013p245 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Phosphorylation of MAPK family proteins by C-type natriuretic peptide (CNP) in LbetaT2 gonadotrophs – a novel cyclic GMP-independent effect?

Jonas Kim , Thompson Iain , Chand Annisa , Fowkes Rob

CNP, the third member of the mammalian natriuretic peptide family, exerts the majority of its biological effects via the guanylyl cyclase B (GC-B) receptor, resulting in accumulation of intracellular cGMP. Previous studies reveal numerous inhibitory effects of natriuretic peptides on the MAPK family in bone, neuronal and cardiovascular tissues. However, we recently reported that CNP stimulates ERK, p38MAPK and JNK phosphorylation in pituitary GH3 somatotrophs. As CNP is known ...

ea0013p246 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

A subnormal peak cortisol response to stimulation testing does not predict a subnormal cortisol production rate (CPR)

Paisley Angela , Rowles Susannah , Roberts Margaret , Brandon David , Trainer Peter

In patients with pituitary disease stimulation tests, such as the insulin tolerance test, are performed as tests of the HPA axis and widely used as the basis for commencing hydrocortisone replacement therapy.We conducted a pilot study to investigate the relationship between the peak cortisol response to insulin-induced hypoglycaemia and daily CPR. Isotopic CPRs were studied in 10 patients (5 male, mean age 44±13 years) with pituitary disease and a s...

ea0013p247 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Sleep disorders and response to treatment in the ‘Acromegalic cardiovascular and respiratory outcomes with primary analogue therapy (ACROPAT)’ trial

Webb Alison , Pilsworth Sam , O’Toole Sam , Strey Chris , Ariyaratnam Shaumya , George Emad , Majeed Joohi , Melvin Alison , Morrish Nick , Roland Jonathan , Smith Ian , Wood Diana , Chatterjee Krishna , Simpson Helen , Shneerson John , Gurnell Mark

Respiratory disorders are a major cause of illness and impaired physical function in patients with acromegaly, contributing to 25% of all recorded deaths, with respiratory mortality 3-fold higher than in normal subjects. Sleep apnoea (SA), the phenomenon of recurrent cessation or decrease of airflow to the lungs during sleep, is the commonest reported respiratory complication. Both obstructive (OSA) and central (CSA) sleep apnoeas are known to occur, although the overall preva...

ea0013p248 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

RNA interference as a therapy for a model of Cushing’s disease

Munir Alia , Eltobgi AbdulAdim , Abbott Lee , Newell-Price John

Introduction: Pro-opiomelanocortin (POMC) is a precursor polypeptide which is cleaved to make adrenocorticotrophic hormone (ACTH) in the anterior pituitary. It is activated in Cushing’s disease. Current therapies for Cushing’s disease are inadequate. RNA interference (RNAi) allows highly specific and effective suppression of gene expression by use of short interfering RNAs (siRNAs).Method: In the current study we employed custom designed siRNAs...

ea0013p249 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Sleep disorders in craniopharyngioma patients – a preliminary report

Crowley Rachel , Costello Richard , Thompson Christopher

Objective: Our aim was to identify and treat sleep disorders in asymptomatic patients with craniopharyngioma and to identify co-existing respiratory disease.Methods: 5 patients (1 male) with a diagnosis of craniopharyngioma, were identified from the local pituitary database and admitted for polysomnography (Alice 4, Respironics), as part of a study of hypothalamic disease in craniopharyngioma. Sleep disturbance was defined as an apnoea index (AHI) of &#6...

ea0013p250 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Small vessel remodelling and impaired endothelial-dependent dilatation in subcutaneous resistance arteries in patients with acromegaly

Paisley Angela , Izzard Ashley , Heagerty Tony , Cruickshank Kennedy , Trainer Peter

Patients with acromegaly die prematurely from CVD. Hypertension is more prevalent with increased peripheral vascular resistance thought to be due to structural remodelling of resistance vessels.We investigated structural properties and reactivity of small arteries in 20 healthy controls(55±11 years, 10 m) and 43 patients with acromegaly (55±14 years, 29 m), split into active disease (AD, n=17, 56±15 years, 13 m) or remission (CD, <i...

ea0013p251 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

Upward resetting of osmostat – rare or under diagnosed?

Chowdhury Sharmistha Roy , Evans PMS , Penney MD , Page MD

Two patients presented to us with polyuria and polydipsia.Case 1– A 34 year old gentleman was referred with polyuria, polydipsia, diffuse headaches and poor sleep. Initial plasma and urine osmolality were 306 and 272 milliosmol/kg respectively. Water deprivation test demonstrated normal concentrating ability. Starting urinary osmolality was 788 milliosmol/kg, rising to 877 milliosmol/kg. MRI pituitary was normal. Hypertonic saline infusion test prod...

ea0013p252 | Neuroendocrinology and behaviour (including pituitary) | SFEBES2007

AIP and familial acromegaly

Gueorguiev Maria , Lolli Francesca , Leontiou Chrysanthia , Chapple Paul , Quinton Richard , Ribeiro-de-Oliveira Antonio , Gadelha Monica , Popovic Vera , Monson John , Wass John , Frohman Lawrence , Grossman Ashley , Korbonits Márta

Acromegaly is almost always due to a sporadic growth-hormone secreting pituitary adenoma, but familial acromegaly has been reported occasionally. Linkage and loss of heterozygosity studies have suggested that it is caused by a tumour suppressor gene located at 11q13. Recently mutations have been identified in a gene in some families with acromegaly alone or acromegaly and prolactinoma. The gene codes for the aryl hydrocarbon receptor interactive protein (AIP), a molecular chap...