Searchable abstracts of presentations at key conferences in endocrinology

ea0094p55 | Metabolism, Obesity and Diabetes | SFEBES2023

Investigating chemogenetic manipulation of spinal astrocytes within a high-fat diet-induced hyperglycaemic model during neuropathic pain development

Hardowar Lydia , Hulse Richard

The spinal cord dorsal horn acts as a hub for the modulation of sensory information, relying heavily upon the interplay of a heterogenous cell population (endothelial cells, astrocytes) with sensory neurons. We have identified in rodent models of hyperglycemic neuropathic pain that spinal microvasculature is damaged. A pathological hallmark of chronic pain is astrogliosis, acting as a potent source of inflammatory response and impacting vessel integrity and sensory neuronal ac...

ea0077p175 | Metabolism, Obesity and Diabetes | SFEBES2021

Pressure from pericytes: angiotensin II induced neuropathic pain development as a complication of hypertension

Hardowar Lydia , Sheavyn Matt , McTernan Philip , Bates Dave , Hulse Richard

The renin angiotensin system (RAS) is targeted as means of medical intervention for hypertensive associated complications of stroke, heart failure and obesity. Ultimately, the inhibition of Angiotensin II(ANGII)/Angiotensin II type 1 (AT1) receptor complex has been key in reducing raised blood pressure. Diabetic neuropathic pain (NP) is highly prevalent in the United Kingdom, associated with an increasingly aged population. There is growing acknowledgment that the vascular com...

ea0086p48 | Metabolism, Obesity and Diabetes | SFEBES2022

Spatial proteomics of skeletal muscle isolates high-fat diet produced inflammatory events

Hardowar Lydia , Narayanan Jayakumar , Rutella Sergio , Hulse Richard , Doig Craig

Metabolic dysfunction in skeletal muscle disturbs both the critical vascular network and fundamental muscle fibre architecture. However, the molecular drivers during metabolic stress responsible for transmitting these events remain poorly defined. To reveal these we mapped changes in the spatial proteome occurring as a result of impaired metabolic health. We exposed male and female mice (C57/BL6J) to high fat diets and conducted digital spatial profiling (NanoString GEOMX) on ...

ea0086p201 | Metabolism, Obesity and Diabetes | SFEBES2022

Angiotensin II dependent pericyte activation causes neuropathic pain in a type-2 rodent model of diabetes

Hardowar Lydia , McTernan Philip , Bates David , Hulse Richard

Neuropathic pain (NP) is a microvascular complication affecting between 30-50% of people living with diabetes. Current painkillers including non-steroidal pain killers and anti-convulsant are ineffective. At the level of the spinal cord (SC), capillary regression is associated with the onset of NP in diabetic rodent models. Our aim was to investigate angiotensin II mediated pericyte activation in relation to vascular degeneration and the onset of NP. Adult male C57Bl/6J mice w...

ea0094oc6.2 | Metabolism, Obesity and Diabetes | SFEBES2023

An hif-fective connection? unravelling the link between hif1α, diabetic neuropathy, and obesity

Younis Awais , Hardowar Lydia , Doig Craig , Hulse Richard

Obesity, a complex metabolic disorder characterised by excessive adipose tissue accumulation, is associated with numerous health complications. This study aimed to investigate the molecular mechanisms underlying obesity, focusing on the role of hypoxia-inducible factor 1 alpha (HIF1α). Our findings establish a novel link between HIF1α and the pathogenesis of obesity-related conditions. We uncovered a connection between HIF1α and diabetic neuropathy, a prevalent ...