Searchable abstracts of presentations at key conferences in endocrinology

ea0065pl10 | Society for Endocrinology Jubilee Medal Lecture | SFEBES2019

Pituitary cells alive: hormone genes pulsing on and off

Davis Julian

Technical advances frequently lead to surprises. Studies of gene expression have used reporter genes since the 1980s to assess how mammalian gene promoters are activated or repressed. Two of the most widely used reporters are firefly luciferase and green fluorescent protein, and both have the advantage that their expression can be seen, and their bioluminescence or fluorescence readily measured. In studying how hormone genes were controlled in the pituitary, we used quantitati...

ea0021sb1.3 | How to access funds and revolutionise your research | SFEBES2009

Mathematical modelling and endocrine research

Davis Julian

Endocrine systems are dynamically changing and complex systems. Quantitative measurements of hormones over time became possible with the development of sensitive immunoassays, and it rapidly became clear that hormone release is often pulsatile. The timing of ultradian hormone pulses and circadian variation turns out to be a central characteristic of many systems, and this has required increasing use of mathematical analysis.Regulation of gene expression ...

ea0021p201 | Endocrine tumours and neoplasia | SFEBES2009

Choroidal malignant melanoma in a patient with Carney complex

Farook Seleena , Gibson Christine , Davis Julian

We report a case of Carney complex who demonstrated most of the disease-defining features and an unusual manifestation of choroidal malignant melanoma not previously reported in the literature.A 16-year-old female patient initially presented to the endocrinologists in 1991 with secondary amenorrhea and raised prolactin levels, diagnosed to have a pituitary microprolactinoma. Past history included episodes of vague syncope and palpitations treated as comp...

ea0015p247 | Pituitary | SFEBES2008

Cyclical Cushing's: a diagnostic challenge

Balakrishna Prasanna Rao , Gibson Christine , Davis Julian

Some patients with Cushing’s have only periodic hypercortisolism, known as cyclical Cushing’s, becoming eucortisolaemic or even transiently subnormal between periods of hypercortisolaemia. We report two cases and discuss the management.A 23-year-old lady presented in March 2007 with Cushingoid features. Initial urinary free cortisol was raised at 594 nmol/l. A midnight cortisol of 568 nmol/l and post-48 h low dose dexamethasone cortisol of 351 ...

ea0038p170 | Neoplasia, cancer and late effects | SFEBES2015

A young girl with bilateral adrenal tumours

Koubeh Sakina , Jinadev Pushpa , Alhelfi Moayed , Davis Julian

18 year old female referred with 2 years history of hirsutism and secondary amenorrhoea. She was born at 37 weeks and was found to have neonatal hypothermia, hypoglycaemia and crossed hemihypertrophy, but no macroglossia. Due to the increased risk of Wilm’s tumour she had regular ultrasound scans up to the age of 11 years. She attained menarche at the age of 13 but had only two periods within 18 months. By the age of 15 she developed hirsutism and acne. Biochemistry revea...

ea0025p79 | Clinical biochemistry | SFEBES2011

A case of lymphocytic hypophysitis

Krishnan Simmi , Davis Julian , Pal Piyali , Gnanalingham Kanna , Wu Fredrick

A 22-year-old pregnant lady presented to the Emergency Department (ED) at 36 weeks of gestation with sudden onset of severe headache and blurring of vision. On examination, she was alert, conscious (GCS: 15/15) and had bitemporal hemianopia, confirmed on formal visual field assessment. MRI brain scan (limited views due to pregnancy) showed a pituitary mass extending into the suprasellar region and reaching the optic chiasm. Serum prolactin (PRL) was elevated at 3876 μ/l (...

ea0039ep82 | Miscellaneous/other | BSPED2015

Development of a feasible intervention to support communication with young people

Callery Peter , Downing Jenny , Gleeson Helena , Clayton Peter , Davis Julian , Dimitri Paul , Wales Jerry , Young Bridget

Background: Many young people have inadequate follow up because they lose contact with adult care following transfer from paediatrics. There is a need to adapt communication interventions to help young people to determine and enact their preferred involvement in consultations with health professionals.Objective: To develop interventions to support communication with young people in endocrine care and to assess the feasibility of implementation in routine...

ea0028p240 | Pituitary | SFEBES2012

Transcriptional regulation of prolactin in the rat oestrous cycle

Patist Amanda , Featherstone Karen , Spiller David , Semprini Sabrina , McNeilly Judith , McNeilly Alan , Mullins John , White Michael , Davis Julian

Circulating levels of prolactin are subject to acute and long-term regulation by many factors including oestrogen and dopamine. We have studied the regulation of prolactin promoter activity in living pituitary cells using transgenic Fischer rats in which reporter gene expression is regulated by the human prolactin gene locus (hPRL-d2EGFP). We have previously identified pulsatile prolactin transcription patterns in living lactotroph cells in fetal tissue, that became stabilised...

ea0025p244 | Pituitary | SFEBES2011

BAC recombineering to understand the role of the alternative promoter in the regulation of prolactin expression

Awais Raheela , McNamara Anne , Harper Claire , Adamson Anthony , Spiller Dave , Semprini Sabrina , Mullins John , Davis Julian , White Michael R H

Alternative promoters control many genes including prolactin (PRL), which in man is expressed at extra-pituitary sites controlled by an alternative promoter located 5.8 Kbp upstream of the pituitary transcription start site. Previous studies using short promoter fragments may be misleading, as the human hPRL genomic locus has many conserved far-upstream regions. To study the function of the alternative promoter, we engineered a bacterial artificial chromosome (BAC) expressing ...

ea0021p293 | Pituitary | SFEBES2009

Long-term unsynchronised transcriptional cycles in individual living pituitary cells

Harper Claire , Finkenstadt Barbel , Woodcock Dan , Friedrichsen Sonke , Semprini Sabrina , Spiller Dave , Mullins John , Rand David , Davis Julian , White Mike

Gene expression in living cells is dynamic and unstable, and fluctuations in transcription may be subject to stochastic regulation of processes including transcription factor and polymerase recruitment, and chromatin remodelling. One gene that has been shown to display dynamically variable transcription and marked heterogeneity between cells is prolactin (PRL). Time-lapse imaging of PRL-reporter gene expression in single rat pituitary GH3 cells revealed distinct long-term tran...