Searchable abstracts of presentations at key conferences in endocrinology

ea0081p543 | Adrenal and Cardiovascular Endocrinology | ECE2022

Whole blood transcriptomic profile of Cushing’s syndrome

Armignacco Roberta , Daniel De Murat , Jouinot Anne , Bouys Lucas , Perlemoine Karine , Letourneur Franck , Adoux Lucie , Zennaro Maria-Christina , Bertherat Jerome , Assie Guillaume

Background: Cushing’s syndrome, caused by an excess of circulating glucocorticoids, is associated with high morbidity and presents high inter-individual variability. The earlier the diagnosis, the better the treatment effectiveness and the prognosis. Hormone assays, routinely used, contribute to identify Cushing’s syndrome. However, no biomarker is currently available to directly quantify the biological action of glucocorticoids. Blood samples represent an easily obt...

ea0093oc26 | Oral communication 4: Pituitary and Neuroendocrinology | EYES2023

Whole blood transcriptomic signature of Cushing’s syndrome

Birtolo Maria Francesca , Armignacco Roberta , Benanteur Nesrine , De Murat Daniel , Guignat Laurence , Groussin Lionel , Libe Rossella , Letourneur Franck , Bertherat Jerome , Jouinot Anne , Assie Guillaume

Background: Cushing’s syndrome (CS) is associated with high morbidity and presents high interindividual variability. Easily measurable biomarkers, in addition to the hormone assays currently used for diagnosis, could better quantify the individual biological impact of glucocorticoids. The aim of this study is to identify such biomarkers through the analysis of whole blood transcriptome.Methods: Whole blood transcriptome was evaluated in 57 samples (...

ea0090oc6.3 | Oral Communications 6: Endocrine-related Cancer | ECE2023

Tumor microenvironment of adrenocortical carcinoma dissected by single-cell RNA-sequencing

Jouinot Anne , Martin Yoann , Foulonneau Thomas , Bendjelal Yanis , Calvet Philip , Violon Florian , Sibony Mathilde , Daniel De Murat , Armignacco Roberta , Perlemoine Karine , Letourneur Franck , Izac Brigitte , Andrieu Muriel , Berthon Annabel , Ragazzon Bruno , Groussin Lionel , Libe Rossella , Bertherat Jerome , Assie Guillaume

Background: Molecular classification is important for diagnosis and prognosis of adrenocortical tumors (ACT). Transcriptome profiles separate benign ACT (“C2” cluster) from carcinomas (ACC) and identify two groups of ACC, “C1A” (“steroid” and “proliferation” signatures) and “C1B” (“immune” signature), of poor and better prognosis respectively. However, these signatures were characterized at the tissue level (“bul...

ea0070yi12 | Young Investigators | ECE2020

Identification of transcriptome profiles in paraffin samples using 3’ RNA-sequencing for the prognostic assessment of adrenocortical carcinoma

Jouinot Anne , Sibony Mathilde , Jeanpierre Lindsay , Septier Amandine , De Murat Daniel , Armignacco Roberta , Perlemoine Karine , Izac Brigitte , Letourneur Franck , Ragazzon Bruno , Leroy Karen , Gaujoux Sebastien , Dousset Bertrand , Groussin Lionel , Libe Rossella , Bertherat Jerome , Assié Guillaume

Background: Adrenocortical cancer (ACC) is an aggressive tumor with heterogeneous prognosis. Previous genomic studies have demonstrated the importance of molecular classification for the prognostic assessment. Among molecular markers, transcriptome profiles “C1A” (steroid and proliferative signature) and “C1B” (immune signature) show the strongest association with outcome. However these markers are determined so far only from frozen tissue samples, sinc...

ea0070aep6 | Adrenal and Cardiovascular Endocrinology | ECE2020

Integrated genomics reveals different subgroups of primary bilateral macronodular adrenal hyperplasia (PBMAH)

Vaczlavik Anna , Bouys Lucas , Letouze Eric , Perlemoine Karine , Guignat Laurence , Letourneur Franck , Sibony Mathilde , Bonnet Fidéline , Heurtier Victor , Espiard Stéphanie , Assié Guillaume , Ragazzon Bruno , Bertherat Jerome

Introduction: Primary bilateral macronodular adrenal hyperplasia (PBMAH) are benign adrenocortical disease responsible for benign tumors and cortisol autonomous secretion. There is a broad spectrum of clinical, imaging and hormonal presentations. Aberrant membrane receptor expression is frequent, the most characteristic example being the food dependent Cushing syndrome due to ectopic expression of the GIP receptor (GIP-R). In 20 to 25 % of these patients, inactivating heterozy...

ea0093oc24 | Oral communication 4: Pituitary and Neuroendocrinology | EYES2023

Transcriptome in paraffin samples for the diagnosis and prognosis of pituitary neuroendocrine tumors (PITNETS)

Benanteur Nesrine , Villa Chiara , Birtolo Maria Francesca , Jouinot Anne , De Murat Daniel , Letourneur Franck , Gaillard Stephan , Raffin-Sanson Marie-Laure , Emile Jean-Francois , Bertherat Jerome , Baussard Bertrand , Assie Guillaume

An initial multi-omics analysis of PitNETs has refined histological classifications, and could improve diagnostic and prognostic assessment (Neou, Cancer Cell 2020). Of all omics, transcriptome best discriminates between these different classes. This molecular classification has been built upon frozen samples, which are difficult to use in routine clinical practice.Aim: Demonstrate the feasibility of measuring the transcriptome in Formalin-Fixed Paraffin...