Searchable abstracts of presentations at key conferences in endocrinology

ea0044p32 | Adrenal and Steroids | SFEBES2016

Discovery of putative aldosterone-regulating microRNAs by analysis of in vitro and in vivo microRNA profiles

Ab Razak Nur Izah , MacKenzie Scott , Robertson Stacy , McBride Martin , Freel Marie , Davies Eleanor

Approximately 10% of essential hypertension cases is attributed to primary aldosteronism (PA), where inappropriately high levels of aldosterone are secreted. Almost half of these PA cases result from aldosterone-producing adenoma. microRNAs are single-stranded, short non-coding RNAs that negatively regulate gene expression post-transcriptionally. In a previous study, we showed that microRNAs directly modulate CYP11B2 (aldosterone synthase) gene expression and aldoster...

ea0038p402 | Steroids | SFEBES2015

Bioinformatic analysis of microRNAs associated with aldosterone secretion

Ab Razak Nur Izah , MacKenzie Scott M , Diver Louise A , McBride Martin M , Davies Eleanor

Many cases of hypertension are associated with inappropriately high levels of aldosterone secretion and it has been proposed that microRNAs play a role in this dysregulation. Previously, we showed that microRNAs expressed within the adrenal cortex significantly repress aldosterone production. Furthermore, stimulation of aldosterone secretion by three different means in the H295R cell line – the most commonly-used in vitro model of the human adrenal cortex – ...

ea0037oc11.3 | Obesity | ECE2015

microRNA profiling of H295R cells following stimulation of aldosterone production: a bioinformatic study

Razak Nur Izah Ab , MacKenzie Scott , Diver Louise , McBride Martin , Davies Eleanor

Inappropriately high secretion of aldosterone can lead to hypertension and its various cardiovascular consequences. We previously showed that microRNA (miRNA) plays a significant role in the regulation of aldosterone biosynthesis. In this study we measured miRNA levels in H295R cells, the most commonly-used human adrenocortical cell line, and investigated how these change in response to the stimulation of aldosterone biosynthesis.H295R cells (n=...

ea0034p364 | Steroids | SFEBES2014

Bioinformatic analysis of altered microRNA production in normal adrenal tissue and aldosterone-producing adenoma

Razak Nur Izah Ab , Diver Louise , Alvarez-Madrazo Samantha , Robertson Stacy , McBride Martin , Stewart Paul , Connell John , MacKenzie Scott , Davies Eleanor

Hypertension is a common risk factor for cardiovascular disease and up to 15% of hypertensive patients are now known to have primary aldosteronism (PA), where the adrenal glands secrete inappropriately high levels of the steroid hormone aldosterone. Of these cases, almost half are due to the presence of unilateral aldosterone-producing adenoma (APA). Aldosterone is synthesised in the zona glomerulosa of the adrenal cortex, with the final stages of production catalysed by aldos...