Searchable abstracts of presentations at key conferences in endocrinology

ea0032p648 | Male reproduction | ECE2013

Characterization of sumoylated proteins in human sperm

Marchiani Sara , Ricci Beatrice , Tamburrino Lara , Muratori Monica , Cambi Marta , Nosi Daniele , Forti Gianni , Baldi Elisabetta

SUMOylation is a post-translational protein modification involved in the regulation of essential cell functions. Our group found several SUMO-1 and SUMO-2/3ylated proteins in human ejaculated spermatozoa (Marchiani et al. 2011). We showed that SUMO-1 is mainly present in live spermatozoa and the percentage of SUMOylated spermatozoa was inversely correlated with total and progressive motility. SUMOylated proteins are mainly located in the nucleus and in the midpiece. T...

ea0032p656 | Male reproduction | ECE2013

CATSPER calcium channels in human spermatozoa and their role in responsiveness to progesterone (P)

Tamburrino Lara , Marchiani Sara , Cambi Marta , Muratori Monica , Forti Gianni , Baldi Elisabetta

CATSPER is a family of sperm-specific calcium channels activated by P in human spermatozoa (Lishko et al. 2011, Strunker et al. 2011). KO mice for CATSPER are infertile due to severe defects in sperm motility.We studied the involvement of CATSPER in human sperm motility and P responsiveness.Western blot analysis with an anti-CATSPER1 antibody demonstrated the presence of three major bands corresponding to CATSPER1...

ea0032p667 | Male reproduction | ECE2013

Sperm DNA fragmentation as assessed by TUNEL/PI: mean values in fertile men and intra individual variability

Muratori Monica , Tamburrino Lara , Cambi Marta , Marchiani Sara , Natali Ilaria , Noci Ivo , Maggi Mario , Forti Gianni , Baldi Elisabetta

Tests detecting sperm DNA fragmentation (SDF) could be included in the clinical management of male infertility. TUNEL/PI is an innovative version of the TUNEL assay, and it is able to distinguish between two different populations: PI brighter and PI dimmer sperm. The aims of the study were to determine the average value of SDF in recruited men of proven fertility and to verify whether SDF discriminates between fertile and subfertile men independently from semen quality. In add...