Searchable abstracts of presentations at key conferences in endocrinology

ea0006oc30 | Neuroendocrinology | SFE2003

Circulating Cocaine- and Amphetamine-regulated transcript (CART) peptide in subjects with abnormalities of the hypothalamo-pituitary axis

McDermott F , Stanley S , Murphy K , Ghatei M , Bloom S

Cocaine and amphetamine regulated transcript (CART) is a hypothalamic neuropeptide with a role in the hypothalamo-pituitary adrenal axis (HPA) in rodents. However, the role of pituitary CART is poorly understood. Previous work in rodents has shown pituitary CART is regulated by corticotrophin-releasing hormone (CRH) and glucocorticoids. CRH increased pituitary CART release fifteen-fold. Adrenalectomised rats had significantly higher pituitary CART mRNA expression, pituitary CA...

ea0005p191 | Neuroendocrinology and Behaviour | BES2003

Problematic development of an immunometric assay for cocaine and amphetamine regulated transcript (CART) (55-102) peptide fragment

Darch S , Chapman R , Murphy K , Bloom S

Obesity is a life-threatening, yet common condition that often lies at the root of other serious illnesses such as diabetes, cardiovascular disease and other neuroendocrine disorders. The central nervous system signalling pathways regulating food intake are complex and the precise mechanisms remain to be determined. Cocaine- and amphetamine- regulated transcript (CART) is a CNS neuropeptide involved in the regulation of feeding.Development of a two-site immunometric assay ...

ea0003p186 | Neuroendocrinology | BES2002

The pattern of green fluorescent protein (GFP) expression following intrahypothalamic injection of adeno-associated virus-GFP

Ward H , Gardiner J , Kong W , Murphy K , Bloom S

Adeno-associated virus (AAV) has been used to express GFP in a variety of tissues. AAV has a tropism for neurones and we have been using AAV-GFP to determine the anatomical localisation of gene transfer after injection into the hypothalami of Wistar rats. The long term aim is to use this viral vector as a tool for investigating the hypothalamic control of appetite, energy expenditure and pituitary function.Male Wistar rats were anaesthetised and injecte...

ea0009p43 | Growth and development | BES2005

Kisspeptin-10 stimulates the hypothalamic-pituitary-gonadal axis in adult male rats following central and peripheral administration

Thompson E , Patterson M , Murphy K , Smith K , Dhillo W , Todd J , Ghatei M , Bloom S

Kisspeptin is the peptide product of the KiSS-1 gene and endogenous agonist for the GPR54 receptor. The kisspeptin/GPR54 system has recently been suggested as a key regulator of the reproductive system. GPR54 deficient mice have abnormal sexual development and low circulating gonadotrophins and in humans, GPR54 mutations have been shown to cause hypogonadotrophic hypogonadism. Thus, GPR54 appears to be essential for normal gonadotrophin secretion and the regulation of puberty....

ea0005oc30 | Brain and Behaviour | BES2003

Elevated circulating levels of cocaine- and amphetamine regulated transcript (CART) in anorexia nervosa

Stanley S , Connan F , Small C , Murphy K , Todd J , Ghatei M , Treasure J , Bloom S

The peptides, cocaine- and amphetamine-regulated transcript (CART), alpha-melanocyte-stimulating hormone (alpha-MSH) and glucagon-like peptide-1 (GLP-1) are present in the circulation and may act as peripheral anorectic signals. CART, alpha-MSH and GLP-1 may play a role in tumour anorexia, but their roles in anorexia nervosa (AN) are unknown. These studies aimed firstly to measure plasma CART, alpha-MSH and GLP-1 in control and AN subjects and secondly, to measure plasma CART ...

ea0005oc32 | Brain and Behaviour | BES2003

Localisation and regulation of cocaine- and amphetamine-regulated transcript (CART) in the anterior pituitary gland

Murphy K , Stanley S , Bewick G , Kong W , Gardiner J , Ghatei M , Small C , Bloom S

Cocaine and amphetamine regulated transcript (CART) was originally isolated from rat brain but CART is also synthesized and stored in the anterior pituitary. The localisation and factors regulating pituitary CART synthesis are largely unknown.Using a specific assay for the pituitary form of CART, CART(55-102), CART-immunoreactivity was detectable in the medium of the corticotroph cell line, AtT-20 (1.73 ± 0.54 fmols per millilitre per 24h) but not in control medium....

ea0003oc43 | Neuroendocrinology | BES2002

CGRP-alpha inhibits food intake via the paraventricular nucleus (PVN) of the hypothalamus partly through the CGRP-type 1 receptor

Dhillo W , Small C , Russell S , Seth A , Murphy K , Ghatei M , Bloom S

CGRP-alpha is a 37 amino acid peptide generated by alternative splicing of calcitonin gene transcripts. CGRP-alpha is present in a number of areas of the brain involved in the regulation of appetite, including the PVN. CGRP(8-37) is a competitive antagonist of CGRP-alpha at the CGRP-type 1 receptor. CGRP-alpha is known to inhibit food intake when given intracerebroventricularly, but the anatomical localisation of this effect is not known. Aims To investigate the effects of CGR...

ea0002oc17 | Neuroendocrinology | SFE2001

The novel hypothalamic peptide Galanin-Like Peptide (GALP) stimulates food intake in male wistar rats

Seth A , Stanley S , Dhillo W , Murphy K , Ghatei M , Bloom S

Galainin-like peptide (GALP) is a recently identified hypothalamic neuropeptide expressed in the arcuate nucleus. It is structurally related to galanin, GALP amino acid residues 9-21 are identical to the N terminal 1-13 residues of galanin. GALP binds to galanin receptors with high affinity but has greatest affinity to the GAL R2 receptor. Galanin has previously been demonstrated to significantly increase food intake when administered into the third ventricle or the paraventri...

ea0005oc31 | Brain and Behaviour | BES2003

A role for arcuate cocaine and amphetamine regulated transcript in hyperphagia, thermogenesis and cold adaptation

Kong W , Stanley S , Gardiner J , Abbott C , Murphy K , Seth A , Connley I , Ghatei M , Stephens D , Bloom S

The hypothalamic peptide, cocaine and amphetamine regulated transcript (CART) was originally reported to act as an anorexigenic peptide. However, we have recently shown that CART injection into the hypothalamic arcuate nucleus (ARC), acutely stimulates food intake. Here, we characterise the role of arcuate nucleus CART in the long term regulation of food intake and brown adipose tissue (BAT) using i) repeated intra-arcuate injection of CART peptide [CART(55-102)] and ii) polye...

ea0031p217 | Obesity, diabetes, metabolism and cardiovascular | SFEBES2013

Appetite regulation during a 6-month military tour to Afghanistan

Hill N E , Fallowfield J L , Delves S K , Brett S J , Wilson D R , Frost G , Dhillo W , Bloom S R , Murphy K G

Background: Military personnel on operational deployment commonly lose weight despite the adequate supply of rations. Moderate weight loss (~5% body mass) occurred during the initial phase of a 6-month deployment to Afghanistan without affecting physical fitness. Reasons for this weight loss are presently unknown. We sought to establish whether changes in appetite regulatory hormones contribute to the observed weight loss.Methods: Body mass and body comp...