Searchable abstracts of presentations at key conferences in endocrinology

ea0037gp.18.06 | Pituitary–Basic and IGF-1 | ECE2015

Comparison of high-throughput platforms in evaluation of whole genome miRNA expression profiles in pituitary tissues

Butz Henriett , Darvasi Otto , Szabo Peter M , Liko Istvan , Pongor Lorinc , Czirjak Sandor , Racz Karoly , Patocs Attila

Introduction: There are three principal high-throughput methods that have been widely used to determine whole genome miRNAs expression profiles: i) microarrays, ii) qPCR based arrays, and iii) next generation sequencing (NGS). Our aim was to compare the results obtained from these platforms in normal and adenomatous pituitary samples and to validate the results by an independent sample set using qPCR.Material and methods: Using four normal pituitary (NP)...