Searchable abstracts of presentations at key conferences in endocrinology

ea0090ep29 | Adrenal and Cardiovascular Endocrinology | ECE2023

A new LC-MS/MS method for simultaneous determination of six steroids in urine for the diagnostic workup of primary aldosteronism

Vogg Nora , Kuerzinger Lydia , Remde Hanna , Kendl Sabine , Fassnacht Martin , Kurlbaum Max

Background: The diagnostic workup of primary aldosteronism (PA) is challenging and the current screening tool, aldosterone-renin ratio, is increasingly challenged. Due to interference with immunoassays and the general high variability of plasma aldosterone levels false-positive as well as false-negative results are probably more frequent than previously assumed. Therefore, we aimed at the establishment of a reliable LC-MS/MS method to analyze aldosterone and related steroids i...

ea0081p385 | Endocrine-Related Cancer | ECE2022

PD-1 and PD-L1 immune checkpoint expression - the prognostic impact on adrenocortical carcinoma

Landwehr Laura-Sophie , Sbiera Iuliu , Altieri Barbara , Remde Hanna , Kircher Stefan , Sbiera Silviu , Kroiss Matthias , Fassnacht Martin

Adrenocortical carcinoma (ACC) is a very severe endocrine malignancy with poor prognosis. While cancer immunotherapies have revolutionized the treatment of several cancer entities, the results of initial studies of different immune checkpoint inhibitors in ACC were heterogeneous and clinically substantial responses were observed only in a subset of patients. Expression of immune checkpoint molecules - programmed cell death 1 (PD-1) and its ligand PD-L1 - has been shown to pred...

ea0081p153 | Pituitary and Neuroendocrinology | ECE2022

Subtype-specific pattern of white blood cell differential in endogenous Cushing’s syndrome

Detomas Mario , Altieri Barbara , Chifu Irina , Remde Hanna , Landwehr Laura-Sophie , Sbiera Silviu , Kroiss Matthias , Fassnacht Martin , Deutschbein Timo

Objective: Glucocorticoid excess impairs immune function, thereby predisposing patients with endogenous CushingÂ’s syndrome (CS) to infections. However, it is still not clear whether there is a CS-subtype specific pattern in white blood cell (WBC) and WBC differential (WBCD) count.Methods: Retrospective monocentric cohort study in patients with either overt endogenous CS or adrenal adenomas with autonomous cortisol secretion (ACS), with WBC and WBCD ...

ea0099p14 | Adrenal and Cardiovascular Endocrinology | ECE2024

Improved selectivity of adrenal venous sampling with the use of alternative steroids to cortisol

Alessi Francesco , Costantinescu Georgiana , Peitzsch Mirko , Remde Hanna , Kurzinger Lydia , Schulze Manuel , Lee Myron , Yang Jun , Reinke Martin , Beuschlein Felix , Bornstein Stefan , Lenders Jacques , Pamporaki Christina , Eisenhofer Graeme

Background: Current guidelines recommend adrenal vein sampling (AVS) for the identification of surgically treatable unilateral disease among patients with primary aldosteronism (PA). The cortisol-derived selectivity index (SI) is currently used to assess the success (or selectivity) of adrenal vein catheterization during AVS. However, AVS studies can be non-selective in many cases.Aim: To examine whether the use of SI derived either from 11-deoxycortisol...

ea0099rc3.5 | Rapid Communications 3: Adrenal and Cardiovascular Endocrinology | Part I | ECE2024

The saline infusion test with mass spectrometric measurements of aldosterone in patients tested for primary aldosteronism

Pamporaki Christina , Remde Hanna , Constantinescu Georgiana , Kuerzinger Lydia , Fuss Carmina Teresa , Peitzsch Mirko , Schulze Manuel , Lee Myron , Yang Jun , Mueller Lisa , Williams Tracy Ann , Reincke Martin , Gruber Sven , Beuschlein Felix , Lenders Jacques , Eisenhofer Graeme

Background: Confirmation of primary aldosteronism (PA) with the saline infusion test (SIT) requires accurate measurements of plasma aldosterone, best achieved by mass spectrometry. Performance of the test and appropriate cut-offs remain inadequately defined.Design and methods: This prospective multicenter cohort study involved 451 patients with suspected PA who underwent a seated SIT. Among these, there were 90 and 76 in whom PA was respectively confirmed and excluded based on...

ea0099rc3.6 | Rapid Communications 3: Adrenal and Cardiovascular Endocrinology | Part I | ECE2024

Mismatch repair deficiency and microsatellite instability in adrenocortical carcinoma: Diagnosis, prevalence, and clinical impact

Altieri Barbara , Herterich Sabine , Kircher Stefan , Jahn Arne , Teleanu Maria-Veronica , Lippert Juliane , Landwehr Laura-Sophie , Kimpel Otilia , Reuter Miriam , Remde Hanna , Stenzinger Albrecht , Glimm Hanno , Bargou Ralf , Froehling Stefan , Ronchi Cristina L , Appenzeller Silke , Fassnacht Martin , Matthias Kroiss

Background: DNA mismatch repair (MMR) maintains genomic integrity and stability. Inactivation of the MMR genes MLH1, PMS2, MSH2, and MSH6 by somatic or germline variants or gene methylation causes MMR deficiency (dMMR) leading to microsatellite instability (MSI) and high tumour mutational burden. In many tumour entities, patients with dMMR/MSI respond well to immune checkpoint inhibitor (ICI) therapy. The clinical relevance of dMMR and MSI i...

ea0099p16 | Adrenal and Cardiovascular Endocrinology | ECE2024

False-positive and false-negative results during screening, confirmatory testing and subtyping for suspected primary aldosteronism: lessons from Prosaldo

Constantinescu Andreea Georgiana , Pamporaki Christina , Alessi Francesco , Passauer Jens , Remde Hanna , Kuerzinger Lydia , Fuss CarminaTeresa , Schulze Manuel , Peitzsch Mirko , Horvath Andrea , Yang Jun , Bruedgam Denise , Williams TracyAnn , Reincke Martin , Gruber Sven , Beuschlein Felix , Lenders Jacques , Eisenhofer Graeme

Background: Diagnostic stratification of patients with suspected primary aldosteronism (PA) is a multistep process reliant on tests that are not infallible. Only through prospective studies can diagnostic accuracy be appropriately assessed.Methods: The PROSALDO trial enrolled 819 patients between 2019 and 2023 to assess steroid profiles against routine tests for diagnostic stratification. A combination of these tests and outcome assessments, including me...

ea0099p89 | Endocrine-Related Cancer | ECE2024

Effectiveness of temozolomide treatment in SDHx mutant and wildtype metastatic pheochromocytoma and paraganglioma – results of a European restrospective multicentre study

Remde Hanna , Hadoux Julien , Crona Joakim , Libe Rossella , Lim Eugenie , Di Dalmazi Guido , Arvat Emanuela , Haissaguerre Magalie , Canu Letizia , Kaltsas Gregory , Igaz Peter , Timmers Henri , Noelting Svenja , Quinkler Marcus , Deutschbein Timo , Fassnacht Martin , Matthias Kroiss

Background: Pheochromocytomas and paragangliomas (mPPGL) are rare neuroendocrine tumors. Therapeutic options in advanced and irresectable mPPGL are limited. Two small retrospective studies demonstrated the effectiveness of temozolomide in patients with mPPGL and suggested that patients with mutation in the succinate dehydrogenase B (SDHB) gene might benefit more than SDHB wildtype cases.Aim: To re-evaluate safety and effectiveness of temozolomide in a la...

ea0099p505 | Endocrine-Related Cancer | ECE2024

Growth differentiation factor 15 (GDF-15) is induced by mitotane in adrenocortical carcinoma and associated with poor prognosis and impaired responsiveness to immunotherapy

Weigand Isabel , Triebig Alexandra , Tanja Maier , Anderlik Tanja , Remde Hanna , Landwehr Laura-Sophie , Kimpel Otilia , Reuter Miriam , Schreiner Jochen , Wedekink Florian , Hoster Eva , Schwarzlmueller Paul , Reincke Martin , Wischhusen Jorg , Fassnacht Martin , Matthias Kroiss

Background: Treatment of adrenocortical carcinoma (ACC) is unsatisfactory in advanced stages. Oral mitotane remains a mainstay of treatment. Response rates of ACC to immune checkpoint inhibition (ICI) are disappointing and markers of response have not been identified. Tumoural infiltration with cells of the adaptive immune system is sparse in ACC tissue. Growth/differentiation factor 15 (GDF-15) is a cytokine that has been described to impair tumoral immune infiltration and is...

ea0081oc13.6 | Oral Communications 13: Adrenal and Cardiovascular Endocrinology 2 | ECE2022

Machine Learning models for the accurate prediction of malignant pheochromocytomas and paragangliomas

Pamporaki Christina , Berends Annika MA , Filippatos Angelos , Prodanov Tamara , Meuter Leah , Prejbisz Aleksander , Beuschlein Felix , Fassnacht Martin , Timmers Henri , Noelting Svenja , Abhyankar Kaushik Ganesh , Contsantinescu Georgiana , Kunath Carola , Wang Katharina , Remde Hanna , Januszewicz Andrzej , Robledo Mercedes , Lenders Jacques , Kerstens Michiel , Pacak Karel , Eisenhofer Graeme

Introduction: Pheochromocytomas and paragangliomas (PPGLs) exhibit an up to 20% malignancy rate. Various clinical, genetic, and pathological features have been proposed as predictors of malignancy. However, until present there are no robust indices to reliably predict metastatic PPGLs.Aim: The aim of the present study was to prospectively validate the value of methoxytyramine as risk marker of metastatic disease and establish a machine learning (ML) mode...