Endocrine Abstracts

Background: Soluble alpha klotho (sαKL) is a circulating protein that has been linked to the growth hormone (GH) axis. We previously showed its association to disease activity in patients with acromegaly, with considerable robustness towards biological confounders. However, there is scarce data in literature regarding the analytical performance of the assay, and pre-analytical stability of sαKL in blood samples. Objective: We aimed to evaluate ...

Background: We recently have shown a close association of high concentrations of soluble alpha klotho (sαKL) to disease activity in acromegaly. Small pilot studies suggested that sαKL concentrations might be reduced in GH deficiency (GHD) and increase after recombinant human GH (rhGH) therapy. Our aim was to evaluate the potential of sαKL as a biomarker in GHD.Methods: We evaluated sαKL in comparison to the classical biomarkers GH, IG...

Background: Recent studies reported high soluble α-klotho concentrations (α-klotho) in active acromegaly, with normalization after successful therapy. We showed that α-klotho correlates to GH and even better to IGF-I. So far, most studies employed an immunoassay from Immuno-Biological Laboratories (IBL) for measurement of α-klotho, but other assays are available. Agreement between assays remains questionable since α-klotho exists in several isoforms: K...

Background: Serum soluble alpha klotho (sαKL) is a circulating protein which had been shown to be very high in active acromegaly, and significantly reduced after disease control. Therefore, sαKL has been suggested as a new biomarker for growth hormone (GH) excess. In this study, we aimed to evaluate the impact of short-term stimulation and suppression of GH and acute modulation of glucose metabolism on sαKL concentrations.Methods: Serum s&...

Background: Alpha klotho is a transmembrane protein that regulates calcium metabolism. Its soluble portion has been linked to endocrine functions. We have recently shown high concentrations of serum soluble alpha klotho (sαKL) in active acromegaly. There is limited information about regulation of sαKL secretion. We aimed to gain insight about sαKL as a potential biomarker for H-related diseases.Methods: SαKL was measured by ELISA (IBL...

Background: Soluble alpha klotho (sαKL) is a peptide hormone, that has been shown to be elevated in serum of patients with uncontrolled acromegaly. One early pilot study suggested that sαKL is also detectable in urine from patients with acromegaly, and significantly drops after surgery. Notably, in this study, urine sαKL concentrations were undetectable in the control group, and fell consistently below the detection limit of the ELISA in postoperative patients i...