Searchable abstracts of presentations at key conferences in endocrinology

ea0038p415 | Steroids | SFEBES2015

Lineage conversion of human cells to an adrenocortical phenotype: a new technology to study the adrenal gland

Babot Gerard Ruiz , Hadjidemetriou Irene , Ajodha Sharon Jane , Taylor David , Taylor Norman , Guasti Leonardo

The adrenal cortex is the primary site of steroid synthesis, producing glucocorticoids under the control of the hypothalamic–pituitary axis and mineralocorticoids under the control of the renin–angiotensin system.Adrenal insufficiency, which can be life threatening, is cause by a number of adrenal disorders, and lifelong management of these patients with exogenous steroids can be challenging. No drug suitably mimics the diurnal pattern of corti...

ea0044p4 | Adrenal and Steroids | SFEBES2016

Generation of human urine-derived steroidogenic cells through lineage conversion: A new technology to study the adrenal gland

Ruiz-Babot Gerard , Hadjidemetriou Irene , Ajodha Sharon Jane , Ghataore Lea , Taylor David , Taylor Norman , Balyura Mariya , Bornstein Stefan R , Guasti Leonardo

Cellular reprogramming describes the process where a fully differentiated, specialized cell type is induced to transform into a different cell. Cell reprogramming techniques can become powerful tools for modelling diseases, drug testing and for personalized cellular therapy. The adrenal cortex is the primary site of steroid synthesis. Adrenal insufficiency, which can be life threatening, is caused by a number of adrenal disorders, and lifelong management of these patients with...

ea0044p33 | Adrenal and Steroids | SFEBES2016

What is the most appropriate cut-off for post-saline aldosterone in saline suppression test after adrenalectomy?

Ranasinghe Ruvini , Taylor David , Whitelaw Benjamin , Aylwin Simon , Vincent Royce

Introduction: Primary aldosteronism (PA) is the most common endocrine cause of hypertension affecting up-to 10% of hypertensives. Saline suppression, a confirmatory test for PA helps avoiding patients undergoing invasive lateralisation procedures due to a false positive aldosterone-to-renin ratio (ARR). The proposed cut-off to exclude PA is post-saline aldosterone suppression to <140 pmol/l. We reviewed our biochemical work-up in order to optimise laboratory assessment.</p...

ea0099ep451 | Thyroid | ECE2024

Thyroxine intolerance: a rare but significant clinical entity

Nawaz Asif , Williams David , Taylor Peter , Min Thinzar , Yin Win

Introduction: Levothyroxine, the standard therapy for hypothyroidism, is usually well tolerated and very few cases of true allergy to levothyroxine have been reported to date. Here we report two cases of rare occurrence of levothyroxine allergy.Case 1: A 56-year-old woman with no previous history of allergies started levothyroxine post-thyroidectomy for Graves’ disease. She developed facial flushing and urticarial rash within 15 minutes of the first...

ea0086p159 | Adrenal and Cardiovascular | SFEBES2022

An audit of the clinical utility of urine steroid profiling for endocrine disorder diagnosis in a routine clinical laboratory in 2021

Taylor David , Churchus Richard , Collins Heather , Ajaj Nicola , Ekundayo Susan , Alramadhi Ulfat , Rayner Oliver , Ghataore Lea

Measurement of steroid metabolites in urine by gas chromatography-mass spectrometry remains the gold standard for biochemical diagnosis of steroidogenic disorders. In the UK, three laboratories offer steroid profiling for routine clinical testing, with the Supraregional Assay Service at King’s College Hospital being the largest and experiencing increased demand year-on-year for testing. In this study, we used our profiling database to audit 2021 workload. By direct compar...

ea0065p305 | Neuroendocrinology | SFEBES2019

Can a hypernatraemia alert system protect inpatients with diabetes insipidus?

Hakim Yasmin , Hoque Nahid , Gurung Ankit , Taylor David , Rao Nandini , Whitelaw Ben

Background: Over the past decade there has been increased recognition of the dangers associated with inpatient management of diabetes insipidus (DI) and omission of desmopressin leading to hypernatremia. In May 2009 a patient died from diabetes insipidus mismanagement in a London hospital. An NHS England patient safety alert was issued in 2016 highlighting this risk.Method: Inpatients with a serum sodium of 155 mmol/l or greater were prospectively identi...

ea0044p66 | Clinical biochemistry | SFEBES2016

Audit of plasma catecholamines vs. plasma metanephrines: experience at a tertiary endocrine referral centre

Taylor David R , Alexander Alex , Schweitzer Adam , Stone Colin , Whitelaw Ben , Aylwin Simon , Vincent Royce P

Background: Phaeochromocytoma and paraganglioma (PPGLs) are rare neuroendocrine tumours arising from the adrenal medulla and paraganglia. Biochemical assessment relies on demonstrating elevated concentrations of catecholamines and their metabolites. Analytical methods for catecholamine and metanephrine measurement vary in sensitivity and specificity. We reviewed our biochemical work-up in order to optimise patient diagnosis.Methods: This retrospective au...

ea0044p79 | Clinical biochemistry | SFEBES2016

Immunoassay cortisol day curve dangerously overestimates cortisol reserve in a metyrapone treated patient

Leong Christine H M , Taylor David R , Gilbert Jackie , Whitelaw Benjamin C

Background: Metyrapone is commonly used in medical management of Cushing’s syndrome. It inhibits 11-β hydroxylase, which catalyses the conversion of 11-deoxycortisol to cortisol. The adequacy of metyrapone blockade can be assessed either clinically or biochemically using a target mean serum cortisol 150–300 nmol/l. Cortisol is normally measured by immunoassay.Case report: A 21-year-old female presented with clinical and biochemical feature...

ea0044p80 | Clinical biochemistry | SFEBES2016

Cortisol measurement using immunoassay versus liquid chromatography-tandem mass spectrometry: metyrapone dose-related discrepancies in cortisol values

Leong Christine H M , Taylor David R , Whitelaw Benjamin C , Aylwin Simon

Background: Metyrapone inhibits 11-β hydroxylase and causes a subsequent rise in the cortisol precursor, 11-deoxycortisol. Cortisol measurements by immunoassays are susceptible to interference and reagent antibody cross-reactivity with cortisol precursors when used in patients receiving metyrapone treatment. Clinicians rely on clinical and biochemical features of cortisol excess for dose titration of this medical blockade. The extent of this interference remains unclear. ...

ea0099ep278 | Diabetes, Obesity, Metabolism and Nutrition | ECE2024

The effects of different weight-loss interventions on Bile acid and FGF-19 metabolism and correlation with metabolic improvement

Behary Preeshila , Alexiadou Kleopatra , Mare Tracey , Walters Julian , Taylor David , Vincent Royce , Tan Tricia

Background: Bariatric surgery, dietary restriction and gut hormones treatment for obesity, lead to distinctive changes in weight-loss maintenance and glycaemia. We previously showed that Roux-en-Y Gastric Bypass (RYGB) and Very-Low-Calorie-Diet (VLCD) were superior in weight-loss, compared to a tripeptide hormone infusion of G lucagon-like Peptide-1, O xyntomodulin and P eptide-YY over a month (GOP, infused at doses to replicate their peak post-prandial concen...