Searchable abstracts of presentations at key conferences in endocrinology

ea0029p762 | Endocrine Disruptors | ICEECE2012

The effects of BPA exposure on fat mass and serum leptin concentrations have no impact on bone mineral densities in non-obese premenopausal women.

Liu J. , Zhao H. , Bi Y. , Ma L. , Zhang L. , Zhao L. , Tao B. , Sun L. , Wang T. , Zhao Y. , Wang W. , Xu M. , Chen J. , Ning G.

Objective: Bisphenol A (BPA) exposure may promote obesity, but its effect on bone mineral density (BMD) has not been reported in humans. In the present study, we aimed to examine the complex interplay between BPA exposure, fat mass, fat-free mass, serum estradiol, leptin, osteocalcin levels and BMDs in a group of premenopausal women.Methods: A total of 246 Chinese premenopausal women aged 20 years and older with regular menstrual cycles were investigated...

ea0009p71 | Growth and development | BES2005

Effect of grape procyanidins on free radical-induced oxidative damage in MC3T3-E1 Osteoblast-like cells in vitro

Zhong J , Zhang L

Objective: To study the protective effect of grape procyanidins on hydrogen peroxide induced oxidative damage in MC3T3-E1 osteoblast-like cells. Methods: Cultured MC3T3-E1 Osteoblast-like cells were treated with grape procyanidins and 125 micromole per litre hydrogen peroxide for six hours. The levels of apoptosis, mitochondrial transmembrane potential and proliferation were subsequently analyzed by flow cytometry and Bio-Red model 550 instrument. Results: The proliferation le...

ea0011p862 | Thyroid | ECE2006

Comparison of the effects of thyrotropin receptor (TSHR) activation on preadipocyte cell lines from white (WAT) and brown (BAT) adipose tissue

Zhang L , Paddon C , Baker GC , Ludgate M

Adipogenesis is induced when intracellular cAMP increases and follows cell cycle arrest. During preadipocyte differentiation expression of the TSHR, which signals via cAMP and the inositol phosphate cascade, is upregulated. To investigate the role of TSHR activation in adipogenesis, we have developed an in vitro model in which constitutively active TSHR mutants (L629F, M453T) are introduced by retroviral vectors (RV).We aimed to compare the effect...

ea0019oc33 | Thyroid, reproduction and endocrine tumours | SFEBES2009

Thyrotropin receptor (TSHR) activation increases hyaluronan (HA) production in preadipocyte-fibroblasts; explanation for HA accumulation in thyroid dysfunction?

Zhang L , Bowen T , Paddon C , Webber J , Giles P , Steadman R , Ludgate M

The thyrotropin receptor (TSHR) is expressed in the thyroid and in precursors undergoing lineage-specific differentiation, e.g adipogenesis. The TSHR is activated by TSH, thyroid stimulating antibodies (TSAB) and gain-of-function mutations (TSHR*). To investigate the role of extra-thyroidal TSHR activation we compared the gene-expression profiles of non-modified human subcutaneous preadipocytes with the parallel TSHR* population. Twenty-seven genes were significantly upregulat...

ea0011p707 | Reproduction | ECE2006

Testosterone restores diabetes-induced erectile dysfunction and sildenafil responsiveness in two distinct animal models of chemical diabetes

Filippi S , Morelli A , Vignozzi L , Zhang XH , Luconi M , Donati S , Forti G , Maggi M

Objectives: Hypogonadism is often associated with diabetes mellitus and both conditions represent major risk factors for erectile dysfunction (ED). The aim of this study was to evaluate the effect of diabetes-induced hypogonadism on ED using two distinct animal models: alloxan-rabbits and streptozotocin (STZ)-rats.Methods: A subgroup of diabetic animals was Testosterone (T) replaced. After 8 weeks from induction of diabetes, erectile function was evaluat...

ea0029p787 | Endocrine tumours and neoplasia | ICEECE2012

Expression of a novel isoform of thyroid peroxidase (TPO) gene in breast cancer (BC)

Muller I. , Giani C. , Fiore E. , Belardi V. , Sabatini S. , Rosellini V. , Funel N. , Campani D. , Grennan-Jones F. , Zhang L. , Lewis M. , Bakhsh A. , Roncella M. , Ghilli M. , Pinchera A. , Vitti P. , Dayan C. , Ludgate M.

Introduction: BC patients have a high prevalence of anti-TPO-autoantibodies (TPOAb), which seem to be protective in BC patients. We hypothesized the presence of T-lymphocytes citotoxicity against a common antigen between thyroid and BC cells.Aim: To evaluate the possible expression of TPO gene in BC.Materials: Frozen tissue specimens: 8 BC, 5 peri-tumoral breast tissues (PT), 3 pancreatic adenocarcinoma (P), 2 kidney cancers (K) an...